Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-afﬁnity substrate for H+/peptide cotransporters pdf

Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... the aqueous buffer. Qualitative analysis of secondary chemicalshift and paramagnetic relaxation enhancement data in conjunction withdynamic information from heteronuclear NOEs and structural ... the calculated structures was acceptable; 89% of the analysed backbone torsions fell into the mostfavoured and additionally allowed regions of the Rama-chandran plot. An additional 6% of residues ... components atnos for automated NOESY cross-peak picking and NOE signalidentiﬁcation [77], candid for NOESY cross-peak assign-ment and calibration of NOE-derived upper distance con-straints [78], and...

Tài liệu Báo cáo khoa học: "Arabic Tokenization, Part-of-Speech Tagging and Morphological Disambiguation in One Fell Swoop" pdf

... Buckwalter Arabic MorphologicalAnalyzer, but (in analysis mode) produces an outputin the lexeme -and- feature format (which we need for our approach) rather than the stem -and- afﬁx format of the Buckwalter ... system of afﬁxation and clitics and the omission of disam-biguating short vowels and other orthographic di-acritics in standard orthography (“undiacritized or-thography”). On average, a word form ... TE1 and TE2 be-cause of the differences in the two parts of the ATB,both in terms of origin and in terms of data prepara-tion.We use the ALMORGEANA morphological ana-lyzer (Habash, 2005), a...

Báo cáo khoa học: Functional classiﬁcation of scaffold proteins and related molecules pptx

... that are important inpathways activated by internal signals, such as DNAdamage, e.g. MDC1 in DNA doule-strand breaks [20] and BRCA1 in many cellular pathways including DNArepair [29]. In a ... proteins).This classiﬁcation has both advantages and limita-tions. Its major advantage is simpliﬁcation, because itenables one to think in terms of a few concepts instead of a practically unmanageable ... revised.AbbreviationsAKAP, A- kinase anchoring protein; DAPP, dual-adapter for phosphotyrosine and 3-phosphoinositides; DD, death domain; FADD,Fas-associated protein with death domain; MAGUK, membrane-associated...

Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc

... proteases [Erv -A, -B and –C (isolated from thelatex of Ervatamia coronaria in our laboratory) and papainfrom Carica papaya (Merck, Kenilworth, NJ, USA)], twoserine proteases [trypsin and chymotrypsin ... CT ex114 aa208 aa24 aaBamH34 aaStartStopXho1N-Pro Protease domainProtease domainCT - ex380 aa1IINP114 aa208 aaBamH134 aaStartStopXho1SS SSGSSSSN-Pro356 aaIIIMSTLFIISILLFLASFSYAMDISTIEYKYDKSSAWRTDEEVKEIYELWLAKHDKVYSGLVEYEKRFEIFKDNLKFIDEHNSENHTYKMGLTPYTDLTNEEFQAIYLGTRSDTIHRLKRTINISERYAYEAGDNLPEQIDWRKKGAVTPVKNQGKCGSCWAFSTVSTVESINQIRTGNLISLSEQQLVDCNKKNHGCKGGAFVYAYQYIIDNGGIDTEANYPYKAVQGPCRAAKKVVRIDGYKGVPHCNENALKKAVASQPSVVAIDASSKQFQHYKSGIFSGPCGTKLNHGVVIVGYWKDYWIVRNSWGRYWGEQGYIRMKRVGGCGLCGIARLPYYPTKAAGDENSKLETPELLQWSEEAFPLAIV A B66 ... coronaria.Biosci Biotechnol Biochem 62, 1947–1955.15 GuhaThakurta P, Biswas S, Chakrabarti C, Sundd M,Jagannadham MV & Dattagupta JK (2004) Structuralbasis of the unusual stability and substrate...

Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt

... Tat proofreading and TMAO reductaseactivity assaysThe signal peptide binding-dependent Tat proofreadingactivity of TorD can be isolated and assayed in vivo,asdescribed previously [5]. Strain ... GTPase activity(Fig. 1B). Titration of increasing amounts of MgCl2gradually enhances GTPase activity, with a peak atapproximately 1 mm (Fig. 1B). The malachite greenassay was also performed ... was thereforeinvestigated by analytical ultracentrifugation.Sedimentation velocity analysis was performed on a sample taken from the initial nickel-afﬁnity chroma-tography and this material...

Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt

... mutational analysis has been carried out onImI, PnIA, PnIB and to a lesser extent on GID and MII.Analysis of ImI has revealed that Asp5-Pro6-Arg7 and Trp10 are important for biological activity ... of Queensland, Brisbane, QLD, Australia a- Conotoxins that target the neuronal nicotinic acetylcho-line receptor have a range of potential therapeutic applica-tions and are valuable probes for ... feature of an arginine residue atposition 12 (Table 1) appears to contribute to a4 b2anda7subtype activity but not a3 b2 activity. A decrease in a4 b2 and a7 subtype activity but not a3 b2 activity was...

Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspeciﬁc alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf

... fraction).BSA (b, 68 kDa), alcohol dehydrogenase (a, 141 kDa) and catalase (250 kDa) wereloaded on to a separate gradient as mole-cular mass markers.K. Komaru et al. Novel aggregate formation of an alkaline ... Inactiva-tion of two mouse alkaline phosphatase genes and establishment of a model of infantile hypophosphatasia.Dev Dyn 208, 432–446.K. Komaru et al. Novel aggregate formation of an alkaline phosphatase ... Hayashi Z, Watanabe A & Hirayama T(1994) Shimada T. Novel missense and frameshift muta-tions in the tissue-nonspeciﬁc alkaline phosphatase genein a Japanese patient with hypophosphatasia....

Tài liệu Báo cáo khoa học: Site-directed mutagenesis of a loop at the active site of E1 (a2b2) of the pyruvate dehydrogenase complex A possible common sequence motif docx

... DCPIPassay, the E1aY28 1A and E1aR28 2A mutants displayed a catalytic activity about twice that of wild-type E1. The singlemutants E1aR26 7A and E1aD27 6A and the multiplemutants E1aY28 1A/ R28 2A/ S28 3A ... 16-foldmolarexcessofdi-domainoverE1.Lane1:E1wild-type;lane2:E1wild-type + di-domain; lane 3: E1aF26 6A mutant; lane 4:E1aF26 6A mutant + di-domain; lane 5: E1aR26 7A mutant; lane 6:E1aR26 7A mutant + di-domain; lane 7: E1aD27 6A mutant; lane 8:E1aD27 6A ... mutantscompared with wild-type E1. However, in the PDH assay,the catalytic activity fell to about 50% of the wild-type value for the E1aD27 6A and E1aR28 2A mutants and to about25% for the E1aY281A...

Tài liệu Báo cáo khoa học: Physico-chemical characterization and synthesis of neuronally active a-conotoxins docx

... identiﬁcation of post-translational modiﬁcationsIsolation and identiﬁcationStandard procedures for identiﬁcation and isolation of a- conotoxins generally incorporate separations usingreversed-phase ... ambiguitiesarising from, for example, asparagine to aspartic acid, orglutamine to glutamic acid changes [14]. The a- conotoxinsEpI, PnIA, GIC, GID, AnIA and AnIB contain pairs of asparagine residues [5,10,21,23,24] ... EpI,PnIA, PnIB, AnIA, AnIB and AnIC and some ana-logues [21,24,56]. Most of the reported syntheses of PnIA, PnIB and the [A1 0L]PnIA analogue have been of the unsulfated forms [51,58–61]. A modiﬁed...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... picolinicacid (PA), quinolinic acid (QA) and NAD homeostasis. Indeed, the enzymestands at a branch point of the tryptophan to NAD pathway, and deter-mines the ﬁnal fate of the amino acid, i.e. transformation ... Walker AL, Iwaki H, Hasegawa Y & Liu A (2005) Kinetic and spectroscopic characterization of ACMSD from Pseudomonas ﬂuorescens reveals a penta-coordinate mononuclear metallocofactor. J Am ... that may prove to be of potentialinterest to reduce life-threatening complications of cerebral malaria, and as an important tool in validat-ing our proposal of hACMSD as a novel drug targetfor...

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