... the aqueous buffer. Qualitative analysis of secondary chemical shift and paramagnetic relaxation enhancement data in conjunction with dynamic information from heteronuclear NOEs and structural ... the calculated structures was acceptable; 89% of the analysed backbone torsions fell into the most favoured and additionally allowed regions of the Rama- chandran plot. An additional 6%...
Ngày tải lên: 18/02/2014, 06:20
... Buckwalter Arabic Morphological Analyzer, but (in analysis mode) produces an output in the lexeme -and- feature format (which we need for our approach) rather than the stem -and- affix format of the Buckwalter ... system of affixation and clitics and the omission of disam- biguating short vowels and other orthographic di- acritics in standard orthography (“undiacritized or- thogra...
Ngày tải lên: 20/02/2014, 15:20
Báo cáo khoa học: Functional classification of scaffold proteins and related molecules pptx
... that are important in pathways activated by internal signals, such as DNA damage, e.g. MDC1 in DNA doule-strand breaks [20] and BRCA1 in many cellular pathways including DNA repair [29]. In a ... proteins). This classification has both advantages and limita- tions. Its major advantage is simplification, because it enables one to think in terms of a few concepts instead of a practi...
Ngày tải lên: 06/03/2014, 22:21
Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc
... proteases [Erv -A, -B and –C (isolated from the latex of Ervatamia coronaria in our laboratory) and papain from Carica papaya (Merck, Kenilworth, NJ, USA)], two serine proteases [trypsin and chymotrypsin ... CT ex 114 aa 208 aa 24 aa BamH 34 aa Start Stop Xho1 N -Pro Protease domain Protease domain CT - ex 380 aa 1 II NP 114 aa 208 aa BamH1 34 aa Start Stop Xho1 SS SS G SSS S N-Pro 35...
Ngày tải lên: 14/02/2014, 14:20
Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt
... Tat proofreading and TMAO reductase activity assays The signal peptide binding-dependent Tat proofreading activity of TorD can be isolated and assayed in vivo,as described previously [5]. Strain ... GTPase activity (Fig. 1B). Titration of increasing amounts of MgCl 2 gradually enhances GTPase activity, with a peak at approximately 1 mm (Fig. 1B). The malachite green assay was also...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt
... mutational analysis has been carried out on ImI, PnIA, PnIB and to a lesser extent on GID and MII. Analysis of ImI has revealed that Asp5-Pro6-Arg7 and Trp10 are important for biological activity ... of Queensland, Brisbane, QLD, Australia a- Conotoxins that target the neuronal nicotinic acetylcho- line receptor have a range of potential therapeutic applica- tions and are...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspecific alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf
... fraction). BSA (b, 68 kDa), alcohol dehydrogenase (a, 141 kDa) and catalase (250 kDa) were loaded on to a separate gradient as mole- cular mass markers. K. Komaru et al. Novel aggregate formation of an alkaline ... Inactiva- tion of two mouse alkaline phosphatase genes and establishment of a model of infantile hypophosphatasia. Dev Dyn 208, 432–446. K. Komaru et al. Novel agg...
Ngày tải lên: 19/02/2014, 17:20
Tài liệu Báo cáo khoa học: Site-directed mutagenesis of a loop at the active site of E1 (a2b2) of the pyruvate dehydrogenase complex A possible common sequence motif docx
... DCPIP assay, the E1aY28 1A and E1aR28 2A mutants displayed a catalytic activity about twice that of wild-type E1. The single mutants E1aR26 7A and E1aD27 6A and the multiple mutants E1aY28 1A/ R28 2A/ S28 3A ... 16-fold molarexcessofdi-domainoverE1.Lane1:E1wild-type;lane2:E1 wild-type + di-domain; lane 3: E1aF26 6A mutant; lane 4: E1aF26 6A mutant + di-domain; lane 5: E1aR26...
Ngày tải lên: 20/02/2014, 23:20
Tài liệu Báo cáo khoa học: Physico-chemical characterization and synthesis of neuronally active a-conotoxins docx
... identification of post-translational modifications Isolation and identification Standard procedures for identification and isolation of a- conotoxins generally incorporate separations using reversed-phase ... ambiguities arising from, for example, asparagine to aspartic acid, or glutamine to glutamic acid changes [14]. The a- conotoxins EpI, PnIA, GIC, GID, AnIA and AnIB contain p...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt
... picolinic acid (PA), quinolinic acid (QA) and NAD homeostasis. Indeed, the enzyme stands at a branch point of the tryptophan to NAD pathway, and deter- mines the final fate of the amino acid, i.e. transformation ... Walker AL, Iwaki H, Hasegawa Y & Liu A (2005) Kinetic and spectroscopic characterization of ACMSD from Pseudomonas fluorescens reveals a penta- coordinate mo...
Ngày tải lên: 18/02/2014, 06:20