Báo cáo khoa học: Natriuretic peptides affect Pseudomonas aeruginosa and specifically modify lipopolysaccharide biosynthesis doc

... intrabacterial cAMP and cGMP concentrations The levels of cAMP and cGMP in bacteria exposed to natriuretic peptides were determined using cAMP and a W. Veron et al. Effect of natriuretic peptides on Pseudomonas FEBS ... 253–263. Effect of natriuretic peptides on Pseudomonas W. Veron et al. 5864 FEBS Journal 274 (2007) 5852–5864 ª 2007 The Authors Journal compilation ª 20...

Ngày tải lên: 07/03/2014, 05:20

... (G-CSF) and cortisol. Peptides were solubilized in acidi®ed chloroform/methanol, and partitioned in chloroform/methanol/water. Water- soluble polypeptides were separated by cation-exchange and reversed-phase ... antibacterial peptides; histone; post-translational modi®cation; ubiquitination; tandem mass spectrometry. In innate immunity, antibacterial peptides are important effector...

Ngày tải lên: 08/03/2014, 10:20

... Maaheimo 3 , Pirkko Mattila 2 and Risto Renkonen 1 1 Department of Bacteriology and Immunology, Haartman Institute and Biomedicum, University of Helsinki, Finland; 2 MediCel, Helsinki, Finland; 3 VTT Biotechnology, ... rhamnosylation of g lycoprotein a nd glyco- peptides. Keywords: A-band O-antigen; GDP-4-keto-6-deoxy- D -mannose r eductase(RMD);GDP-rhamnose; Pseudomonas aeruginosa...

Ngày tải lên: 17/03/2014, 11:20

... of chlorophyll biosynthesis Mark Shepherd, Samantha McLean and C. Neil Hunter Robert Hill Institute for Photosynthesis and Krebs Institute for Biomolecular Research, Department of Molecular Biology and ... product and intermediate evolution. All solutions contained 100 m M Tris pH 7.5, and 100 mM NaCl. Immediately after mixing, concentrations of ChlM, SAM and MgD were fixed at 0....

Ngày tải lên: 20/02/2014, 02:21

... polymerase and 2 lL of the template library in a final volume of 20 lL. The amplified enolase PCR product and the pQE30 plasmid vector were digested with KpnIandPstI restriction enzymes, and these ... were screened by PCR and plasmid DNA preparation, and finally sequencing was performed (Mac- rogen Inc., Seoul, South Korea) using standard protocols [20]. Expression in E. coli and pre...

Ngày tải lên: 16/03/2014, 18:20

Ngày tải lên: 23/03/2014, 03:20

... integrates local and global coherence concerns. Summary sentences are grouped before ordering is applied on two levels: group-level and sentence-level. Different algorithms for grouping and ordering ... identification, interpretation, and summary generation (Hovy, 2005). In the simple case of extraction, topic identification and interpretation are conflated to sentence select...

Ngày tải lên: 23/03/2014, 16:20

... from lobster and Saccharomyces cerevisiae, both as apoenzyme structures and complexes with substrates and inhibitors [4–7]. These two enolases have very similar amino-acid sequences and three-dimensional ... developmentally and tissue specifically, but the kinetic properties of all isoenzymes are very similar [14,15]. In yeast, the levels of the two isoenzymes are under metabolic a...

Ngày tải lên: 23/03/2014, 21:20

... bacteria, fungi and protozoa: making it and taking it. Microbiol- ogy 155, 1386–1396. 88 Gardocki ME, Jani N & Lopes JM (2005) Phosphati- dylinositol biosynthesis: biochemistry and regulation. Biochim ... PC and diacyl PE species and a small increase in alk-1-enyl-acyl PE. Changes in the PE and PC contents in (B) and (C) relative to control cells (A) are reflected by the siz...

Ngày tải lên: 28/03/2014, 23:20

... tetraphenylarso- nium and ethidium bromide and it decreases the resistance against divalent cations like dequalinium and methyl viologen [13]. A region including TMS 2 to 5, and homologous to proteins ... spectro- scopy and by functional assays. Experimental procedures 5 Strains, plasmids, oligonucleotides and DNA techniques 6 Strains and plasmids used in this study are listed i...

Ngày tải lên: 30/03/2014, 13:20