Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane pot

... contained a truncated core OS and consisted of a linker (Glc-Kdo 2 )-lipid A structure. The phenotype of this mutant was examined to investigate the roles of different moieties of the LOS molecule ... those of the wild-type, indicating a truncated LOS molecule resulting from the mutant (Fig. 3A). Western blot analysis demonstrated that the LOS from O35Elgt3 l...

Ngày tải lên: 07/03/2014, 05:20

... Furthermore, the status of one binding site could in uence the binding of the regulatory protein to the other, representing cooperativity [5,6]. A regulatory protein may be the product of the ... interaction leading to gene expression. The status of the switch is determined by the state of the upstream regulatory element of the GAL genes in the nucleus....

Ngày tải lên: 07/03/2014, 16:20

... France. Results Evidence of different steps in the GPI-bALP mediated mineralization: effects of Ca 2+ concentration To assess the role of Ca 2+ in initiating the mineralization process, we modelled the biological ... identification by infrared spectra To identify the quality and the type of mineral formation obtained in the mineralization medium during the...

Ngày tải lên: 23/03/2014, 17:21

... mediator of lethal sepsis, triggering the production of proinflammatory cytokines and liver injury [13]. Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform ... USA). Binding of all of the anti- bodies was detected using an ECL detection system (iNtRON Biotechnology, Seoul, Korea) in accordance with the manu- facturer’s...

Ngày tải lên: 14/02/2014, 18:20

... basal membrane proteins to migrate apically. Transcytosis of integrins would therefore enable H. pylori with the intriguing possibility of targeting the integrin b 1 receptor at api- cal membranes ... transcytosis of integrin molecules from the basal to the apical side of the cells, a process that has been suggested for integrin b 1 [15]. Indeed, disrup- tion of host-c...

Ngày tải lên: 14/02/2014, 19:20

... and of the kanamycin insertion mutant nirF strain (SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom left panel and of SBN13 (DnirF) containing pEG276-nirF in the ... activity of NirF Interestingly, like NirN, NirF shares sequence similar- ity with the C-terminal d 1 heme-containing domain of cytochrome cd 1 . Strikingly, the axial lig...

Ngày tải lên: 15/02/2014, 01:20

... role in skin wound healing. After a skin injury, PPARb expression is rap- idly elevated in the epidermis at the wound edges; dele- tion of a single PPARb allele results in delayed wound healing ... in skin barrier disruption (Fig. 1A). PPARb mRNA expression was determined in keratinocytes isolated from the epidermal layer of these skins, and was found to be significantl...

Ngày tải lên: 18/02/2014, 18:20

... amounts of protein from 3Y1-Ad12 cell lysates confirmed the absence of cathepsin D in nontransfected cells, and the presence of both the 31 kDa mature cathepsin D and the 45 kDa procathepsin D in cathepsin ... con- trol of endosomal acidity was confirmed using cellular systems in which modification of the expression levels of cathepsin D, either by transfection of...

Ngày tải lên: 19/02/2014, 02:20

... h after the start of treatment with EGF, indicating that EGF regulated the level of the Bcl-X L protein at the transcriptional level. Next we determined the effect of ADR on the expression of Bcl-X L (Fig. ... proteins, is induced through MAP kinase activation, allowing transactiva- tion of genes containing AP1-binding elements. How- ever, it is not known whether EGF is...

Ngày tải lên: 19/02/2014, 06:20

... concentration. The dissociation constants, K d values, enthalpies of binding and stoichiometries were determined from the binding isotherm by fitting a 1 : 1 binding model to the data using the software ... mutant proteins. This was interpreted as a lack of fosfomycin binding to these mutant proteins [12]. Binding of fosfomycin to either wild-type MurA or the K22 mutant prote...

Ngày tải lên: 19/02/2014, 13:20