Báo cáo khoa học: Unraveling multistate unfolding of pig kidney fructose-1,6bisphosphatase using single tryptophan mutants docx

... The Authors Journal compilation ª 2007 FEBS 5349 Unraveling multistate unfolding of pig kidney fructose-1,6- bisphosphatase using single tryptophan mutants Heide C. Ludwig, Fabian N. Pardo*, Joel ... activity of the nonrecombinant, recombinant wild-type and the mutant pig kidney FBPases as a function of GdmCl Table 1. Kinetic parameters for wild-type and single...

Ngày tải lên: 07/03/2014, 05:20

... y1 w3 h3" alt="" 68 70 72 74 76 78 80 82 84 86 88 10 20 30 40 50 60 70 80 90 precision number of iterations complete-data estimation incomplete-data estimation

Ngày tải lên: 20/02/2014, 18:20

... group of words, many of which are traditionally treated as function words belonging to the realm of grammar rather than to the lexicon. But for a single discourse relation there is often a ... variety of semantic and pragmatic overtones, which render the choice of a marker meaning- driven, as opposed to a mere consequence of structural decisions. Furthermore, a number of...

Ngày tải lên: 20/02/2014, 18:20

... {y t } T t=1 are sequences of in- put and output variables. Respectively, where T is the length of sequence, x t ∈ X and y t ∈ Y where X is the finite set of the input observations and Y is that of the output ... a method of choosing appropriate values for the setting function δ of the active set and for the constant value ω of the inactive set. These two problems are closely rel...

Ngày tải lên: 23/03/2014, 17:20

... of a top-down parser just before the construction of X consists of (the label of) X followed by the sequence of labels on the right siblings of the nodes encountered on the path from X back ... the kind of parser we want to study using G. 1.2 Mappings from trees to trees The transformations presented here can also be un- derstood as isomorphisms from the set of parse tr...

Ngày tải lên: 31/03/2014, 04:20

... precision of 74% and recall of 60%, and K-nearest neighbors in TiMBL: both in (Evans, 2001) with precision of 73% and recall of 69%, and in (Boyd et al., 2005) with precision of 82% and recall of 71%. Since ... learning curve of Figure 1 (left) presents the increase of the performance obtained by Elliphant using the training data randomly ordered. The performance reaches its...

Ngày tải lên: 31/03/2014, 21:20

... regarded as a somehow “de- generate” form of text, unworthy of the same de- gree of attention as the rest of the text. But as we will discuss, the degree of ambiguity in ta- ble understanding is ... patterns of logical relations we have found in the course of empirically analyzing tables from corpora. One advantage of this inventory of data models arises from our appropriati...

Ngày tải lên: 08/03/2014, 02:21

... integrated vision of the catalytic mechanism of both enzymes. The main points are: (a) a re-evaluation of the role of superoxide as a reductant in the catalytic cycle; (b) the existence of Cpd I isomers; ... addressing each of these questions, we will propose a new hypothesis that describes an integrated vision of the catalytic mechanism of MPO and LPO. A brief survey of the...

Ngày tải lên: 20/02/2014, 02:21

... concentrations of GdnHCl (0.25–7 M ) at 25 °C for 1 h. The emission spectra were collected over the range of 310–400 nm using 295 nm as an excitation wavelength. Unfolding of HSA in the presence of bilirubin The ... slope of the transition, DG UÀF H 2 O is the free energy of unfolding in the absence of denaturant. The fluorescence intensity (F) of a protein at equilibrium...

Ngày tải lên: 16/03/2014, 16:20

... rate constants of this RNase A variant correspond to those of wild-type RNase A (Fig. 4), indicating that the local unfolding of the Ala20 loop is independent of the global unfolding of the RNase ... also used to determine the unfolding rate constants of RNase A. Thus, both the local unfolding of this loop (vi a the cleavage a t Ala20–Leu21) and t he global unfolding...

Ngày tải lên: 16/03/2014, 18:20