Báo cáo khoa học: Novel repressor of the human FMR1 gene ) identification ¨ of p56 human (GCC)n-binding protein as a Kruppel-like transcription factor ZF5 ppt

... increased thermostability, appears to have a decreased stability and the temperature optimum is decreased compared with that of the wild-type. Structural analysis of the mutations The effects of the ... isolation of the most active amylosucrase (Asn387Asp) characterized to date, with a 60% increase in activity and a highly efficient polymerase (Glu227Gly) that produce...

Ngày tải lên: 23/03/2014, 11:20

... siRNA ZF5 (5¢-GAGGAAGCAUGA GAAACUCUU-3¢ and 5¢-GAGUUUCUCAUGCUUCCU CUU-3 ) matched bases 945–963; and the third siRNA ZF5 (5¢-GGUCCUGAACUACAUGUACUU-3¢ and 5¢-GUAC AUGUAGUUCAGGACCUU-3 ) matched bases ... regu- lation of the FMR1 gene. A novel human (GCC) n -binding protein, p56, was isolated and identified as a Kru ¨ ppel-like transcription factor, ZF5, by...

Ngày tải lên: 07/03/2014, 05:20

... Chakraborty S, Biswas S, Chakrabarti C & Dattagupta JK (200 5) Crystallization and preliminary X-ray diffraction studies of the cysteine protease erva- tamin A from Ervatamia coronaria. Acta ... merops database (http://merops.sanger.ac.uk); this also contains mammalian intracellular proteases such as cathepsins (B, C, L, K, S, etc .) and proteases from pathogenic parasites,...

Ngày tải lên: 18/02/2014, 16:20

... buffer containing 0.15 m M NADPH and 0.5 m M DXP. H-DXR was added to have an absorbance decrease of about 0.1 min )1 . The rate was measured by following the decrease of the absorbance at 340 nm ... glass funnel containing celite and the tetrahydofuran was removed under reduce pressure. The filtrate was diluted with diethyl ether (50 mL) and the organic layer was washed wit...

Ngày tải lên: 21/02/2014, 03:20

... G & Rosing J (200 1) Snake venom activators of factor X: an overview. Haemostasis 31, 225–233. 8 Takeya H, Nishida S, Miyata T, Kawada S, Saisaka Y, Morita T & Iwanaga S (199 2) Coagulation ... of the heavy chain, the sense primer was 5¢-GCCAAAT(C/T)CAGCCTCCAAA ATG-3¢ and the antisense primer was 5¢-CTGAGAGA AGCCAGTGGTTGA-3¢. To clone its far 3¢ noncoding region, th...

Ngày tải lên: 07/03/2014, 06:20

... efflux of compounds, the parameter k a was calculated. As shown in the Materials Table 2. Parameters characteristic of the interaction of rhodamines with cells. The data are the means ± SEM of three ... also the measurement of the rate of passive diffusion of the dye through the plasma membrane. This cannot be done by the simple measurement of the incr...

Ngày tải lên: 08/03/2014, 08:20

... (the N 2 -fixing rhizo- bia), many of the most abundant bacteria in the oceans (the Roseobacters and the SAR11 clade, includ- ing Pelagibacter) and some laboratory model organ- isms (e.g. Paracoccus, ... result of the different ways in which the proteins interact with heme. Although the proteins have a functionally A CD B Fig. 3. Bioanalytical studies of the associati...

Ngày tải lên: 28/03/2014, 22:21

... version of DEviaNT that uses just the BASIC STRUCTURE features (as a feature ablation study). The SVM models use the same parameters and kernel function as DEviaNT. The state -of- the- practice approach ... function AS( a) is a real-valued measure of how likely an adjective a is to modify a noun ∈ SN. We define AS( a) to be the relative frequency of a in sente...

Ngày tải lên: 07/03/2014, 22:20

... (Char- niak and Elsner, 200 9) is MARS (Mitkov, 199 8) from the GuiTAR (Poesio and Kabadjov, 200 4) toolkit. This algorithm is based on statistical anal- ysis of the antecedent candidates. Another ... freely available systems for AR. Although Charniak and Elsner (200 9) present a machine-learning based algorithm for AR, they evaluate its performance in comparison to three...

Ngày tải lên: 17/03/2014, 00:20

... fraction). BSA (b, 68 kDa), alcohol dehydrogenase (a, 141 kDa) and catalase (250 kDa) were loaded on to a separate gradient as mole- cular mass markers. K. Komaru et al. Novel aggregate formation of an alkaline ... Satomura K, Yamagata M, Shima M, Nakajima S, Mushiake S, Okada S & Ozono K (199 8) Analysis of localization of mutated tissue-nonspecific alkaline phosphatase pr...

Ngày tải lên: 19/02/2014, 17:20