Báo cáo khoa học: Rotenone inhibits mammalian cell proliferation by inhibiting microtubule assembly through tubulin binding Pallavi Srivastava and Dulal Panda ppt

... 4801 Rotenone inhibits mammalian cell proliferation by inhibiting microtubule assembly through tubulin binding Pallavi Srivastava and Dulal Panda School of Biosciences and Bioengineering, Indian Institute ... rotenone inhibits microtubule assembly by induc- ing conformational change in tubulin. Inhibition of proliferation and mitosis Roteno...

Ngày tải lên: 07/03/2014, 05:20

... NF-jB binding Rat aortic muscle cells [70] Human VSMCs [71] Rat VSMCs [72] HUVEC [73] Rat kidney mesangial cells [74] Activated IjB kinases and increased NF-jB binding Human endothelial cells ... Reference Deprivation Human breast cancer cells NF-jB and AP-1 Increased DNA binding [90] Deprivation Human intestinal (Caco-2) cells STAT-4 Increased DNA binding and nuclear protein le...

Ngày tải lên: 07/03/2014, 00:20

... apopto- sis by using 3T3-L1 adipocytes and rat primary adipo- cytes. Adipocyte apoptosis could be induced by troglitazone, pioglitazone or 15-deoxy-D12,14-prosta- glandin J2 (PGJ2), and reversed by GW9662, ... regulators of cell survival, and many studies have demonstrated that activated Akt-1 blocks cellular apoptosis [36]. In human lung carcinoma cells, rosiglitazone suppresse...

Ngày tải lên: 16/02/2014, 09:20

... proteins and DNA, thus damag- ing cell structures and compromising function [4]. When antioxidant mechanisms are overwhelmed by ROS and subsequent oxidative stress occurs, cell damage and cell death ... SB SB 0 20 40 60 80 100 ** ** ** ** ** ** a b c d e f DCF positive cells (%) DCF positive cells (%) Percentage of sub-G1 cells (%) Percentage of sub-G1 cells (%) Fig. 6. Effect...

Ngày tải lên: 18/02/2014, 13:20

... curcumin inhibits microtubule assembly through tubulin binding Kamlesh K. Gupta 1 , Shubhada S. Bharne 2 , Krishnan Rathinasamy 1 , Nishigandha R. Naik 2 and Dulal Panda 1 1 School of Biosciences and ... study suggests that curcumin inhibits cell proliferation and triggers cell killing at least partly by perturbing micro- tubule assembly and function throu...

Ngày tải lên: 19/02/2014, 05:20

... assemblies by comparing the kinetics of fibril formation of native and oxidized proteins. Interactions between amino acid side chains help to stabilize protein structures and control folding and the assembly ... fibril formation for WT and V30M TTR and their oxidized forms were monitored by turbidity measurement at 330 nm and 400 nm. These absorbance measurements detect both fi...

Ngày tải lên: 19/02/2014, 05:20

... these cells by overnight treatment with doxy- cycline (25 ngÆmL )1 ). Cell lysis, immunoprecipitation and immunoblotting Cells were washed with phosphate-buffered saline (NaCl ⁄ P i ) and incubated ... (Hpo) and the scaffold protein Salvador participate in a pathway that controls cell proliferation and apoptosis. Hpo most closely resembles the pro-apoptotic mammalian sterile...

Ngày tải lên: 19/02/2014, 06:20

... essential subunit for assembly of an active mammalian mitochondrial complex I. Experimental procedures Cell lines and cell culture The isolation and preliminary biochemical and genetic characterization ... m M sucrose and 10 m M MgCl 2 ) at a density of 2 · 10 7 cellsÆmL )1 . Cells were permeabilized by digitonin (100 lgÆmL )1 )for  5minat4°C, the cell suspension was dilu...

Ngày tải lên: 19/02/2014, 16:20

... amorphous aggregates and fibrillar deposits. Fig. 5. Aggregation of a-synuclein after 240 h. Samples containing MPTP (A and C) and MPP + (B and D) were analysed by SDS ⁄ PAGE (A and B) on 5–15% crosslinked ... 100 lM MPTP and 50 lM dopamine (F), in the presence of 200 lM MPTP and 50 lM dopamine (G), in the presence of 100 l M MPP + and 50 lM dopamine (H) and in the presence...

Ngày tải lên: 14/02/2014, 19:20

... biology of RhoA- mediated regulation of cell morphology, adhesion and migration in general. Materials and methods Cell lines and reagents The HEK293T cell line was maintained in Dulbecco’s modified ... 15, 30 and 60 min, followed by fixing and staining for extracellular bacteria using fluorescein isothiocyanate (FITC)-labelled Yersinia antibodies (green). Cells were then permea...

Ngày tải lên: 16/02/2014, 15:20