Báo cáo khoa học: Hyperthermophilic enzymes ) stability, activity and implementation strategies for high temperature applications pot

... results and Ta- ble 3 shows the final NER F1 results. We compare to the state-of-the-art methods of Ando and Zhang (200 5), Suzuki and Isozaki (200 8), and for NER—Lin and Wu (200 9). Tables 2 and 3 ... 82.71 Lin and Wu (200 9), 3.4B - 88.44 - Ando and Zhang (200 5), 27M 93.15 89.31 - Suzuki and Isozaki (200 8), 37M 93.66 89.36 - Suzuki and Isozaki (200 8), 1B...

Ngày tải lên: 20/02/2014, 04:20

... 0.181 (+6.3 %) ◦ 0.356 (+2.1 %) ◦ 0.526 (+0.5 %) ◦ Oracle Cluster Order 0.206 (+21.5 %)  0.392 (+12.6 %)  0.597 (+14.0 %)  Oracle Round-Robin 0.206 (+21.5 %)  0.396 (+13.6 %)  0.586 (+11.9 %)  Table ... blocking agent and α- blocker refer to the same class of drugs) and on- tological mismatch (for example, CE might men- tion beta-agonists, while a retriev...

Ngày tải lên: 20/02/2014, 12:20

... in arbitrary units. ( 1) Without OsDmc1 protein; ( 2) 1.25 l M; ( 3) 2.5 lM; ( 4) 5.0 lM; ( 5) 10.0 lM; and ( 6) 12.5 l M of OsDmc1 protein. (B) Effect of deproteinization on strand exchange reaction ... the renaturation (A) and strand exchange (B) reactions mediated by OsDmc1 protein. Phi-C and Phi-W represent rhodamine and fluorescein carrying strands, respectively,...

Ngày tải lên: 19/02/2014, 07:20

... 1) and helices 2 and 3 (Library 2) were randomized and introduced into the chorismate mutase selection system. Second, the successful clones from the initial libraries were crossed (Library 3) ... bundle fold (Fig. 2) [16]. Each monomer consists of three a-helices and two turns. Three libraries were constructed and subjected to in vivo selection for CM activity (Fig....

Ngày tải lên: 19/02/2014, 12:20

... (K) DH m ¢ (kcalÆmol )1 ) DH m c ¢ (kcalÆmol )1 ) DS m ¢ (calÆmol -1 ÆK -1 ) DS m c ¢ (calÆmol )1 ÆK )1 ) T h ¢ (K) T s ¢ (K) DC p ¢ (kcalÆmol )1 ÆK )1 ) 0 340 ± 2 256 ± 2 58 ± 3 )5 8 ± 5 170 ± 9 )2 28 ± 10 ... (B) at 293 K, 3.7 ± 3.3 calÆmol )1 ÆK )1 and 0.7 ± 3 calÆ mol )1 ÆK )1 Æ M )1 , respectively; and, at 318 K, 98 ± 11...

Ngày tải lên: 19/02/2014, 12:20

... equivalent 19. 4). Glc1P (m M )orMD(gÆL )1 ) Activator oxyanion (m M ) v max (UÆmg )1 ) K m (m M ) PL-phosphorylase 1.0–50/80 Phosphite (1 0) 1.86 ± 0.11 11.2 ± 2.0 50/5–120 Phosphite (1 0) 1.95 ± 0.12 ... R242A), neutral (S238A, S224A), or destabilizing (R226A), compared to the wild-type. Except for R226A and R242A mutants (Table 1), all enzymes were st...

Ngày tải lên: 19/02/2014, 16:20

... with PQ for 30 min and its effect on erythrocytes thiobarbituric acid reactive substance (TBARS), protein carbonyl (PCO) as well as non-protein thiols (NPSH) is determined in presence and absence ... solu- tions (0.0 to 0.9 % of NaCl). After gentle mixing and standing for 15 min at room temperature the eryt- hrocytes suspensions were centrifuged at 1500 rpm for 5 min. The a...

Ngày tải lên: 25/10/2012, 11:40

... bonds formed by hydroxyl groups (O–HÆÆÆO,  21 kJÆmol )1 ) are twice as strong as those formed by the amide group [21]. Furthermore, hydrogen bonds formed by the sin- gle hydroxyl donor in Ser and ... Gammarus and Capitella (five acidic groups), Patella (eight basic groups) and Daphnia Amy2 (five basic groups). Secondly, identi- cally charged residues are frequently adjacent (six o...

Ngày tải lên: 14/02/2014, 18:20

... by HSP70 and HSP90. Immunol Lett 117, 9–15. 18 Brand IA & Heinickel A (199 1) Key enzymes of carbo- hydrate metabolism as targets of the 11.5-kDa Zn(2 +)- binding protein (parathymosin). J Biol ... in high- glucose DMEM with 10% fetal bovine serum, 100 UÆmL )1 penicil- lin, 100 lgÆmL )1 streptomycin and 2 mml-glutamine. All cell culture media and reagents were from...

Ngày tải lên: 15/02/2014, 01:20

... 0.4 −50 0 50 100 150 200 250 300 t c(t) c(t) c(t) dc(t)/dt dc(t)/dt dc(t)/dt ABC Fig. 3. The time evolution of c and _ c for the system in Eqn (1 1). The parameter values of k 1 , k )1 , k 2 , k )2 and p and the total ... 0.1 2) is the steady state. Here, we report these eigenvalues: k 1 ¼ )1 732, k 2 ¼ )8 851, k 3 ¼ )1 4 109, k 4 ¼ )6 8 060, k 5 ¼ )1 15 750...

Ngày tải lên: 18/02/2014, 06:20