Báo cáo khoa học: Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts docx

... Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts Rudolf Tischner 1, *, Mary Galli 2, *, ... determine the source of arginine-dependent NOS activity in plants, using crude protein extracts from Arabidopsis leaves. By employing the citrulline-based NOS assay, an argi- nin...

Ngày tải lên: 07/03/2014, 05:20

... with strong binding to the NOS CaM binding domains (Fig. 3). All other mutant CaM proteins failed to either activate or bind the cNOS enzymes. These results indicate that the N-terminal domain ... enzymes during binding and activation [4,5]. The present investigation, designed to further assess the role of the two CaM EF hand pairs in the binding and activation of nitric...

Ngày tải lên: 16/03/2014, 13:20

... enrichment in iNOS activity within the particulate fraction of epithelial cells. Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal ... of iNOS to apical domains of intestinal epithe- lial cells. Particulate fraction association of iNOS in vivo In order to determine the distribution of iNOS in intestin...

Ngày tải lên: 19/02/2014, 16:20

... together with the name, the two extreme points of the chain leaving the intermediate undefined. The entire process, using generic knowledge as the reference point, is shown in Figure 2. Figure ... of the objects while the A-Box contains the set of the assertions on the objects. The former corresponds to the ability of describing by the use of NPs, and...

Ngày tải lên: 18/03/2014, 02:20

... (i.e. the surface form of valency). In this way, the approach is similar to other theories: In the lexical entries of the wordforms there is a pointer to the rules which can be applied with the ... darin, (The nature consists in ) Here in (I) and (3) the subject is obligatory, but in (2) only the direct object. In the other cases the prepositional...

Ngày tải lên: 18/03/2014, 02:20

... serine and gly- cine was reduced from 33% in the light to 25% in the dark (Fig. 7A). Glutamine and asparagine were the major amino acids in the xylem sap in both the light (63% of the total amino ... or glutamine was omitted from the reaction mixture. The kinetics of Fd-GOGAT activity were assayed by increasing the concentrations of Fd isoprotein as indicated in...

Ngày tải lên: 18/02/2014, 18:20

... igestion with SalI (which recognizes the sequence underlined in the oligonucleotides), the DNA fragment was cloned into the pXp2 vector that contains the luciferase reporter g ene. The construct with ... both in vitro and in vivo [38]. To analyse the involvement of the potential GAF and Adf-1 binding sites in activating the a-F1-ATPase promoter, we eliminated th...

Ngày tải lên: 07/03/2014, 16:20

... glycoforms differing in t he postion of a terminal Rha residue occurs in the P. aeruginosa LPS [30], one of them being markedly similar to the Rha-containing glycoform o f the P. syringae LPS core. ... species, that of P. syringae being distinguished by the simultaneous pres- ence of GlcNAc and Rha. The same LPS core composition was revealed by other studies in all P. syri...

Ngày tải lên: 23/03/2014, 13:20

... that GRIP binding to brain SR induces a conformational change that increases the racemase activity by over two-fold. Although the PDZ6 domain of GRIP is involved in the interaction with the last ... at increasing substrate concentrations. The Lineweaver–Burk plot is depicted in the insert. These assays were performed in the presence of 1 m M Ca 2+ and in the abse...

Ngày tải lên: 30/03/2014, 03:20

... and indicating that, in itself, the interaction of ODN with STAT3 induced these effects (i.e. inhibition of the cyclin-D1-dependent promoter, colocalization with STAT3 and STAT3 binding in pull-down ... (particularly in whole ani- mals) by impairing the action of STAT1-dependent interferon. The results obtained in the present study, including the ability of the hai...

Ngày tải lên: 18/02/2014, 08:20