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Báo cáo khoa học: Secondary structure of lipidated Ras bound to a lipid bilayer pptx

Báo cáo khoa học: Secondary structure of lipidated Ras bound to a lipid bilayer pptx

Báo cáo khoa học: Secondary structure of lipidated Ras bound to a lipid bilayer pptx

... structure of Ras at the air–waterinterface was analysed. With the largely increasedsignal -to- noise ratio of ATR-FTIR, we have, for theTable 1. X-Ray and NMR-based secondary structure of Ras ... within an accuracy of 3%, because thesame parameter set was used.Results Lipid bilayer formation and protein adsorptionFor the measurements of membrane -bound lipidated Ras, the lipid layer was ... of membrane -bound Ras protein as a new tool, pavingthe way to revealing the dynamic interactions of mem-brane -bound N -Ras protein with its effectors and regu-lators (i.e. Ras binding domain of Raf,...
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Báo cáo khoa học: Solution structure of Cu6 metallothionein from the fungus Neurospora crassa pptx

Báo cáo khoa học: Solution structure of Cu6 metallothionein from the fungus Neurospora crassa pptx

... A ˚over all backbone atoms. This value isvery similar to t he r.m.s.d. comparison between the a- d omain of mouse MT-1 and the a- domain of rat MT-2,2.14 A ˚[23], suggesting that accurate and ... setswere made and 10 structures calculated for each set, giving a total of 100 structures calculated for each point in Fig. 4.ResultsMetal binding stoichiometry of NcMTN. crassa can express ... mM2,2-dimethyl-2-silapentane-5-sulfonate (asan internal reference) [32], and 0.02% NaN3that wasdegassed prior to protein addition under high vacuum andre-pressurized to 1 atm under argon to avoid oxidation/disproportionation...
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Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf

Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf

... 77–78.28 Sasaki A, Inagaki-Ohara K, Yoshida T, Yamanaka A, Sasaki M, Yasukawa H, Koromilas AE & Yoshimura A (2003) The N-terminal truncated isoform of SOCS3translated from an alternative initiation ... Scrofani2, Manuel Baca1,† and Raymond S. Norton11 Walter and Eliza Hall Institute, Parkville, Victoria, Australia2 Amrad Corporation Ltd, Richmond, Victoria, Australia3 Department of ... is a negative regulator of cytokine signalling that inhibits Januskinase-signal transduction and activator of transcription (JAK-STAT)mediated signal tranduction by binding to phosphorylated...
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Báo cáo khoa học: Secondary structure conversions of Alzheimer’s Ab(1–40) peptide induced by membrane-mimicking detergents pdf

Báo cáo khoa học: Secondary structure conversions of Alzheimer’s Ab(1–40) peptide induced by membrane-mimicking detergents pdf

... disappearance of all NMR peaks at detergentconcentrations of 1–2 mm may have more than oneexplanation. An obvious reason for signal loss is thatFig. 3. The crosspeak signal intensity of assigned ... minima at 208 and 221 nm.The mean residual molar ellipticity at 195 nm as a function of detergent concentration at 3 °C and 20 °Cis shown in Fig. 1C. The disappearance of an initialweakly structured ... suggesting that theaggregates are only partly dissolved after the titration.The situation may be compared to that of a partly-folded molten globule structure of a protein like a- lact-albumin [23]....
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Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... ascomycete fungal laccase fromThielavia arenaria – common structural features of asco-laccasesJuha P. Kallio1, Chiara Gasparetti2, Martina Andberg2, Harry Boer2, Anu Koivula2, Kristiina Kruus2,Juha ... observed for MaL, that may determinethe properties of these asco-laccases at high protein concentrations.DatabaseStructural data are available in the Protein Data Bank database under the accession ... copper-containing enzymes used in various applications, suchas textile bleaching. Several crystal structures of laccases from fungi andbacteria are available, but ascomycete types of fungal laccases...
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Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... peptide atoms) and rjis the standard deviation of Bfactors. The normalized B factors have a zero mean and unitvariance. All atoms that satisfy Bz‡ 4 are treated as outliersand discarded. After ... analogous with other NLS cargo. The averageatomic B factors for importin -a in the structure are32.1 A ˚2for main-chain atoms, 35.7 A ˚2for side-chainatoms and 33.8 A ˚2overall (3244 atoms). ... importin -a nuclear import pathway, provided that CLIC4 can undergo a conforma-tional rearrangement that exposes the NLS in an extended conformation.DatabaseStructural data are available in...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... Nakamura T, Yamamoto T, Abe M, Matsumura H,Hagihara Y, Goto T, Yamaguchi T & Inoue T. (2008)Oxidation of archaeal peroxiredoxin involves a hyperva-lent sulfur intermediate. Proc Natl Acad ... 34 to structure andcatalysis. Biochemistry 48, 3417–3424.31 Nakamura T, Matsumura H, Inoue T, Kai Y, UegakiK, Hagihara Y, Ataka M & Ishikawa K (2005)Crystallization and preliminary X-ray ... sidechain atoms and a water oxygen. One aspartate and two histidine sidechains ligated to the central metal on the equatorial plane. In the Fe -bound form, an additional water molecule was observed...
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... substrate-freeAppA the C a atoms are 2.41 A ˚apart, whereas for thesubstrate-free PhyK and the substrate-loaded AppAthe averaged distance is only 1.87 A ˚.Distinct conformational changes ... groups of HAPs are adapted to different habitats. To support plant growth, bacteriado not need to release phosphate as fast as the diges-tive tract of an animal host, where possible substratesmight ... polypeptidechain is organized into an a and an a ⁄ b domain, and the active site islocated in a positively charged cleft between the domains. Three sulfateions bound to the catalytic pocket of an inactive...
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Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

... delPozo A & Gavilanes JG (2001) RNase U2 and alpha-sarcin: a study of relationships. Meth Enzymol 341,335–351.3 Lacadena J, A ´lvarez-Garcı´ a E, Carreras-Sangra N,Herrero-Gala´n E, Alegre-Cebollada ... inHtA replaces a tyrosine of a- sarcin, and the aromaticring of F126, close to the catalytic H113, replaces a leucine side chain in a- sarcin in a similar arrangement to that found in RNase T1. ... specific activities. Although classic ribotoxinssuch as a- sarcin and restrictocin (about 150 aminoacids) are larger than nontoxic RNases (about 96–110amino acids), they share a similar central structuredregion...
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... growth factorsand proteinase inhibitors. Biol Chem 380, 473–483.17 Kataoka H, Uchino H, Asada Y, Hatakeyama K,Nabeshima K, Sumiyoshi A & Koono M (1997) Analy-sis of tissue factor and tissue ... surface analysis, we propose a rigiddomain association for the N-terminal SEA domain with the back site of the proteinase domain.AbbreviationsHAI, hepatocyte growth factor activator inhibitor; ... supernatantwas clarified and collected, and DESC1 was purified byusing affinity chromatography on a benzamidine columnfollowed by anion exchange chromatography on a Q-Seph-arose column (Amersham...
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