Báo cáo khoa học: ATP-dependent ligases in trypanothione biosynthesis – kinetics of catalysis and inhibition by phosphinic acid pseudopeptides doc
Báo cáo khoa học: ATP-dependent ligases in trypanothione biosynthesis – kinetics of catalysis and inhibition by phosphinic acid pseudopeptides doc
... 540 8–5 421 ª 2008 The Authors Journal compilation ª 2008 FEBS 5421 ATP-dependent ligases in trypanothione biosynthesis – kinetics of catalysis and inhibition by phosphinic acid pseudopeptides Sandra ... concentration of phosphinate and initiating with enzyme.Modality of inhibition The mode of inhibition of the slow-binding phosphi-nate was determined by examining the effect of varyingeach substrate ... understanding of the kinetic and chemical mecha-nism of GspS and TryS involved in the biosynthesis of glutathionylspermidine and trypanothione is crucial forthe design of inhibitors against these...
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Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc
... proceduresCloning and expression of the open readingframes for gld1 and gld2The gld1 gene was cloned from a cDNA library of theH. jecorina strain Rut C-30 [21] by PCR. The followingprimers, introducing ... The GLD1 pro-tein was tagged with a histidine tag at the N-terminalend by adding the coding sequence for six histidines tothe end of the open reading frame, and then expressed in S. cerevisiae. ... activity in crude yeastextract as the untagged protein, indicating that the tagwas not interfering with the protein activity. Thetagged protein was purified and then used for furtheranalysis.In...
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Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf
... proteinsSteffen P. Graether and Brian D. SykesCIHR Group in Protein Structure and Function, Department of Biochemistry and Protein Engineering Network of Centres of Excellence, University of ... protein and i ce at the atomic level and has included the determination of a number of fi sh AFPstructures (Fig. 1 ) (reviews in [ 9–1 6]). Early models of theinteraction between this class of proteins ... face of T mAFP interacts with theice surface.It is important to distinguish whether the m utationsdisrupt the ice–binding interaction by c hanging the surfaceproperties of t he protein, or by...
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Tài liệu Báo cáo khoa học: Antioxidant defences in cybrids harboring mtDNA mutations associated with Leber’s hereditary optic neuropathy docx
... obtained from different clones of thesame cell line, or among data obtained in single controls and in single cell lines affected by the same mtDNAmutation, we decided to carry out the followingexperiments ... blotting analysis of content of CuZnSOD (16 kDa) and MnSOD (25 kDa) proteins in control and LHON-affected cybridcells maintained in glucose-supplemented culture medium (glu-DMEM). Tubulin (55 ... mL)1), and GPx activity was expressed in nmolNADPH consumed per mg proteinÆmin)1.GR activity was measured by the method of Carlberg &Mannervik [44], by following the rate of oxidation of NADPH...
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Tài liệu Báo cáo khoa học: "An Experiment in Evaluating the Quality of Translations" pdf
... original because of the writer’s relative lack of ex- pertness in the Russian language) and sorted by the writer into nine piles of ascending degrees of “informa- tiveness” of the original ... hundred sentences, consisting of nearly all the translations of the sentences in Passage 1, were sorted and re-sorted by the writer into nine piles of increasing intelligibility, so that the ... meaning intended. 1. Not informative at all; no new meaning is added nor is the reader’s confidence in his understanding increased or enhanced. 0. The original contains, if anything, less information...
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Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt
... mechanism to increase the complexity of proteomes is the editing of transcripts (both in nuclear and organelle genomes) [4,5]. Dual targeting of proteins does not increase the number of proteins in acell, ... targeting may occur. Finally, an outline of the future challenges in this field is provided, and the insights that may be gained from a greater under-standing of the mechanism of dual targeting ... mis-sort-ing of proteins between mitochondria and chloroplasts.The structures of binding of targeting peptides tomammalian and plant Tom20s not only revealed themolecular details of binding [54,55],...
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Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf
... residues in the N-domain of ClpA by monitor-ing the binding, unfolding and degradation of several different substrates,including short unstructured peptides, tagged and untagged proteins. Inter-estingly, ... forvarious adaptor proteins [ 6–1 0]. ClpA consists of threedomains: an N-domain and two ATP-binding domainsreferred to as the D1 and D2 domains. Interestingly,deletion of the N-domain from ClpA not ... The amount of peptide remaining was determined by quantification of the Coomassie-stained band following separation of the proteins by Tris ⁄ Tricine SDS–PAGE. (E) The ATPase activity of wild-type...
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Báo cáo khoa học: "Some Problems in the Mechanical Translation of German" pdf
... continue to discuss the solution of these first two types, since it will be contained in that of the main problem, the distinction of nominative from accusative in the feminine and neuter singular ... syntactical function of such forms is ambiguous, the types of ambiguity be- ing three in number. 1. The masculine singular nominative of der and welcher is identical to the feminine singu- lar dative. ... The masculine singular accusative of wel- cher is identical to the dative plural. 3. The nominative form is identical to the ac- cusative in the feminine singular of der and welcher, in the...
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Báo cáo khoa học: The propeptide in the precursor form of carboxypeptidase Y ensures cooperative unfolding and the carbohydrate moiety exerts a protective effect against heat and pressure pot
... to obtain complementary information concerningprotein–solvent interactions [14,15], the unfolded states of proteins [16,17], and protein folding pathways [18].Our analytical techniques involved ... (propeptide) of 91 residues [2,3],compared to the mature CPY. This propeptide structure isessential for folding both in vivo and in vitro,aswellasformaintaining CPY in an inactive form [ 4–6 ]. The ... a single domain, thus ensuringcooperative unfolding. The carbohydrate moiety has aslightly protective role in heat-induced unfolding and ahighly protective role in pressure-induced unfolding.Keywords:...
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Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx
... G-proteininteraction of the active receptor [28]. In summary, Arg46 of the V1aR is required for highaffinity agonist binding and signalling. It is one of theconstraining residues which maintain ... arginyl at this locus is a constrainingresidue which contributes to maintaining the conformationalswitch of the V1aR in the off-state. The unique ability of arginyl to fulfil this constraining ... Critical role of a subdomain of the N-terminus of the V1avasopressin receptor for binding agonists; functional rescue by theoxytocin N-terminus. Biochemistry 39, 1352 4–1 3533.11. Postina, R., Kojro,...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘI