Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf

... found in the operator sequence. RepA contains two domains, one of which binds specifically to the half-repeat. The other is the dimerization domain, which is involved in protein protein interactions ... nm) of free and bound unlabeled C160 RepA and wild-type RepA, with the protein concentration con- stant. The ratio of the apparent F W94 of bound an...

Ngày tải lên: 07/03/2014, 04:20

... of a protein on the basis of the presence of certain dipeptides the occurrence of which is signifi- cantly different in the unstable proteins compared with those in the stable ones [38]. The instability ... reduces the intracellular levels of the core+1 ⁄ S protein in a spe- cific and dose-dependent manner, suggesting an effect of the core protein o...

Ngày tải lên: 30/03/2014, 03:20

... structure of the N-terminal domain of the human homologue, solved at 2.2 A ˚ resolution [11]. The obtained model covers residues 254–568 of HpPex5p, corresponding to the C-terminal half of the protein ... change of the protein. Lowering the pH to 6.0 causes a decrease in the accessibility of the emitting Trp chromophores as suggested by the almost threefol...

Ngày tải lên: 21/02/2014, 00:20

... about the role of these factors in vivo and their role in furunculosis. The A-layer is believed t o play a crucial role in the bacterial protection against c omplement-mediated killing in vitro and ... D 2 O (Table 1). The NH-2a was assigned on the basis of intra- residue NOE with H-1a.TheNH-2c was assigned based on the intraresidue NOEs with H-1c,H-2c and H-...

Ngày tải lên: 23/03/2014, 13:20

... orientations in the membrane; the N terminus of the N-terminal domain is extracellular, while the N terminus of the C-terminal domain is intracellular. In the [st326]2HCT family, TMS I is not part of the ... 100% of the links between the N-terminal sequences of ST3 with scope subfamily, 77% with scope family, and 11% of the links with scope class...

Ngày tải lên: 07/03/2014, 17:20

... sites within the MGP coding sequence (Fig. 2 ). Analysis of the phase of each of the xMGP introns located within the coding region revealed that introns 2 and 3 are of phase I while intron 4 is ... human and mouse MGP genes is split into two exons (IA and IB) in the X. laevis gene, with the site of the intron insertion localized within the 5¢ UTR region of...

Ngày tải lên: 08/03/2014, 10:20

... hydro- gen bonds with the base, was mutated to N in order to see how the increased distance between the base and substrate-binding amino acid affected the binding of dThd and deoxycytidine (dCyd). Results We ... A ˚ in complex with the feedback inhibitor dTTP. The R-fac- tor and R free were 23.2 and 24.2%, respectively (Table 3). Most of the protein cou...

Ngày tải lên: 19/02/2014, 02:20

... TnC-bind- ing site interacts with the C-domain of TnC in both the relaxed and contractile states, which plays a role in maintaining the structural integrity of the troponin complex [17,18]. These ... probably induced by streng- thening of the interaction between the structural TnC- binding site and the C-domain of TnC accompanying Ca 2+ binding to site IV of...

Ngày tải lên: 20/02/2014, 01:20

... hypoxia and emersion showed a  3-fold increase in glucose and a more than 30-fold increase in lactate, whereas the levels in controls remained constant. The arginine kinase gene (AK) and the eukaryotic ... and identifying the native CHH neuropeptide in the ES, after the recent report of CHH cDNA from the ES [28]. Also, we examined the physiological responses...

Ngày tải lên: 16/03/2014, 06:20

... pseudo-threefold symmetry of the three subdomains designated a, b and c, and that the protein has two sugar-binding sites in subdomains a and c [24,25]. Therefore, determination of the sugar-binding ability and specificity ... (Ch; C-half) of EW29 (EW29Ch) has two sugar-binding sites in subdomains a and c, and the protein uses these sugar-binding sites for its...

Ngày tải lên: 23/03/2014, 04:21