Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx

... characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution Stefano Paravisi 1 , Gianluca Fumagalli 1 , Milena Riva 1 , Paola Morandi 1 , ... the discriminating behaviour of Mt-GluRS in E. coli. Studies on Tt-GluRS indicated that discriminating and nondiscriminating GluRS can be distinguish...

Ngày tải lên: 07/03/2014, 03:20

... College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular ... & Ueland PM (1992) Determination of the in vivo redox status of cysteine, cysteinylglycine, homocysteine, and glutathione in human plasma. Anal Biochem 200, 218–...

Ngày tải lên: 07/03/2014, 05:20

... present in certain lin- eages of bacteria, plants, and protozoa but missing in mammals, catalyzes the single-step degradation of sulfur-containing amino acids (SAAs) to a-keto acids, ammonia, and ... concentration of 2 mm) and the kinetic parameters of recombinant EhMGL1 and EhMGL2 (Tables 1 and 2). Despite the heterogeneity of the EhMGL1 preparation used in the pr...

Ngày tải lên: 07/03/2014, 05:20

... carboxyles- terases and albumin, was controlled by nondenaturing PAGE and activity staining [26,68]. Kinetics of substrate hydrolysis in the absence of ligand Kinetic assays with neutral and positively and negatively charged ... on steady-state kinetics The effects of the ligands (L: tyramine, serotonin and ben- zalkonium) on the AAA and esterase activities of wild-typ...

Ngày tải lên: 18/02/2014, 17:20

... 3¢-terminal guanosine of the ribozyme as the first-step nucleophile, whereas self-splicing utilizes an exog- enous guanosine. To further aid in our understanding of ribozyme reac- tions, a kinetic ... earlier, a value of 2.8 · 10 7 Æm )1 Æmin )1 was obtained for the second order rate constant k 2 ⁄ K M . Using this value of k 2 ⁄ K M and the values of k 2 and k )1 (4.1Æmin )1...

Ngày tải lên: 18/02/2014, 18:20

... mitogen-activated protein kinase kinase, in vitro and in vivo: analysis of active mutants expressed in E. coli and generation of the active form in stress response in seedlings. Plant J 29, 637–647. 40 ... protein kinase; MAPKK, mitogen-activated protein kinase kinase; MBP, myelin basic protein; OXI1, oxidative signal-inducible 1; PDK1, 3-phosphoinositide-dependent kinase 1;...

Ngày tải lên: 14/02/2014, 19:20

... miRNAs are single-stranded, nonprotein-coding RNA molecules, approximately 19–25 nucleotides in length. By binding to the complementary sites in the 3¢UTRs of their target genes, they can induce transla- tional ... regulator of the light-dependent resetting of the clock itself. In this study, we have uncovered a role for a cluster of miRNAs in the control of core components...

Ngày tải lên: 18/02/2014, 06:20

... prothrombin already obtains over 70% of its potential binding and protein C mutants II and III each have approximately 50%, whereas wild-type protein C has obtained a mere 19% of its potential binding. ... mem- brane binding affinity enhancement by minor increases in the overall on-rate and ⁄ or minor decreases in the overall off-rate. The results obtained in the present st...

Ngày tải lên: 18/02/2014, 06:20

... gene in the mosquito fat body. The in vitro activation dis- played in AaHNF-4A in hormone-free media that har- bors a complete balance of amino acids suggests the involvement of amino acids in its ... nature of that remains undetermined [72]. Several lines of evidence have sug- gested a role for vertebrate ERRa and ERRc in the control of metabolic genes. Both isoform...

Ngày tải lên: 18/02/2014, 13:20

... occur during the initial 6 h window of infection. We infected RPE cells with K26GFP [27], and stained cells for F-actin, using phalloidin at 30 min and 6 h postinfection, and examined the cells ... fully understand HSV-1 infection of all target cells, includ- ing RPE cells, our study provides new starting points for understanding viral pathogenesis in the retina and advancing no...

Ngày tải lên: 18/02/2014, 14:20