Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt

... enriched in hydrophobic residues. In yeast, proteins targeted to mitochondria Fig. 1. Overview of dual targeting of proteins to mitochondria and chloroplasts in plant cells. A gene encoding a dual- targeted ... mem- brane of chloroplasts (Toc)64 and Toc159 system used by proteins involved in photosynthesis [36]. The Toc159 family in Arabidopsis consi...

Ngày tải lên: 07/03/2014, 03:20

... FEBS accumulation of many proteins involved in photosyn- thesis (termed ‘photosynthetic proteins ), suggesting that both atToc33 and atToc159 are involved in the import of photosynthetic proteins. However, ... carrying a cytosolic preprotein. In the pathway, Hsp90 docks to the TPR repeats of Toc64 before the preprotein is handed over to Toc34 [70]. Certain preproteins, s...

Ngày tải lên: 07/03/2014, 03:20

... domains are depicted as columns. Regions involved in membrane binding are coloured in red, motifs involved in protein protein interaction are blue and the dehydrogenase domains of Tic32 and Tic62 ... four-transmembrane domain proteins. Topology-predictions indicate an N in /C in orientation. Tic62 belongs to the extended family of SDRs and can be divided in two dist...

Ngày tải lên: 07/03/2014, 03:20

... compilation ª 2009 FEBS MINIREVIEW Protein transport in organelles: Protein transport into and across the thylakoid membrane Cassie Aldridge*, Peter Cain* and Colin Robinson Department of Biological Sciences, ... conformation, allowing the transportation of proteins that fold too quickly or tightly for the Sec pathway, or proteins that require the insertion of co-fac...

Ngày tải lên: 07/03/2014, 03:20

... min. Unbound preproteins were washed off and bound preproteins eluted with 250 mm imidazole containing buffer. In every case, precursor protein was detected in the imid- azole eluate, indicating ... binding and hydrolysis [4–7]. Toc34 GDP interacts with the second G -protein in the Toc complex, Toc159, and simultaneously transfers the preprotein to Toc159. Toc159 action facil...

Ngày tải lên: 23/03/2014, 07:20

... hydrophobic TM segments of membrane proteins to exit laterally into the lipid phase. In bacteria, the translocation of secretory and membrane proteins occurs through a homologous channel in the plasma membrane, ... biosynthesis of many proteins [1]. These include soluble proteins, such as those ultimately secreted from the cell or localized to the ER lumen, and membra...

Ngày tải lên: 07/03/2014, 06:20

... SENP2, protein inhibitor of activated STAT (PIAS) and Ran binding protein 2 (RanBP2) [16–20]. The N-terminal loops of SUMO proteins are not homologous to those of ubiquitin and Nedd8, and their ... migration inhibiting factor 2; Nedd8, neural cell-expressed developmentally down-regulation protein 8; Pc2, polycomb protein 2; PIAS, protein inhibitor of activated STAT...

Ngày tải lên: 30/03/2014, 04:20

... analysis of protein transport into pancreatic microsomes in single turnover translocation experiments, and of various other components, believed to b e subunits of protein translocase and involved in ... quantity of protein, present in the respective band of a gel of a certain protein preparation, was determined by comparison with protein standards that were...

Ngày tải lên: 30/03/2014, 15:20

... and a high rate of methylglyoxal formation, hinting that glycation might occur in these cells [21]. Protein glycation by methylglyoxal in yeast, monitored by argpyrimidine formation in proteins, ... growth in 9 days. At this time, cytosolic proteins were extracted and analysed by western blotting. Argpyrimidine-modified proteins were observed in all strains except BY4741...

Ngày tải lên: 30/03/2014, 20:20

... through protein protein interactions. AaHR38 and AaSvp, during the arrest and termination of vitellogenesis, respectively, bind to AaUSP preventing its hetero- dimerization with AaEcR, and, consequently, ... presence of 20E, however, favors the formation of the AaEcR⁄ AaUSP heterodimer [13], indicating that the regulation of the activity of these proteins occurs through...

Ngày tải lên: 18/02/2014, 13:20