Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx

... stabilization of the Toc /Tic/ preprotein supercomplex. In this model, Tic1 10 forms the channel protein and also acts in the recruitment of Hsp93 in concert with the co-chaperone Tic4 0. The TPR domain of Tic4 0 ... 1166–1176 ª 2009 The Authors Journal compilation ª 2009 FEBS MINIREVIEW Protein transport in organelles: The composition, function a...

Ngày tải lên: 07/03/2014, 03:20

... monitored by the reduction of cytochrome c in the presence of NADH at 550 nm [1]. Protein determination Protein content was determined by the Bradford test [20], using bovine serum albumin as standard. PHI ... Lovenberg [23] and Beinert [24], respectively. Kinetic constants The catalytic activity of PHR was evaluated both in the presence and in the absence of...

Ngày tải lên: 21/02/2014, 00:20

... cloning the PCR fragments into the His-tag vector, pET-15b. The recombinant proteins were generated in the E. coli strain Origami (DE3), and the N-terminal His-tagged proteins were purified using ... opening and cell swelling, an event that is probably reduced in extent by CsA. CsA specifically inhibits PTP opening by binding to cyclophilin D in the matrix and on the i...

Ngày tải lên: 16/02/2014, 09:20

... located in independent domains (a b-domain for the N-terminal cluster and an a-domain for the C-terminal cluster). In the absence of metals, apo-thionein (apo-T) is predominantly unstruc- tured and ... composed of a single polypep- tide chain of 61–68 residues. They are characterized by a conserved array of 20 cysteines and the absence of His and aromatic amino a...

Ngày tải lên: 16/02/2014, 15:20

... forming eight b-sheets [23]. The motif was noted on the basis of homology with the C-terminal region of meprins A and B and the TRAF-C domain, and, like the BTB domain, facilitates protein protein interaction ... with CUL3 and other BTB proteins [2,16], it remains unclear what kind of interactions are mediated by the MATH domain in plants. In this article, we sh...

Ngày tải lên: 18/02/2014, 06:20

... answers each. The total cost of producing the corpus was about $350, consisting of $310 paid in workers rewards and $40 in Mechanical Turk fees, including all the trials conducted during the devel- opment ... documents). The source of Wik- iXML is the English part of the Wikipedia XML Corpus by Denoyer and Gallinari (2006). In the original data some of the...

Ngày tải lên: 20/02/2014, 09:20

... suppression of malignant phenotypes, suggesting the role of miR-9 in the growth inhibition of malignant cells. The fundamental function of miRNA is to regulate their targets by direct cleavage of the ... of tumorigenesis, and may have diagnostic and therapeutic value in the future. Materials and methods Materials Four pairs of ovarian tissues, including fou...

Ngày tải lên: 07/03/2014, 00:20

... organelles. Therefore it could be used to study the involvement of these proteinases in protein degradation and the processing of invariant chain. An assay combi- ning a new monospecific CatE antibody and ... distinguishes between the activities of the enzymatically similar proteinases, CatE and CatD, and can therefore be used to investigate the involvement of the...

Ngày tải lên: 07/03/2014, 09:20

... upon the distance between and relative ori- entation of two fluorophores, being independent of the strength of protein protein interactions. In the case of very weak interactions as described in ... citrine) and citrine–GAPDH (chimeric protein with N-terminal citrine and C-terminal GAPDH) were localized in the cytoplasm. PGK–ceru- lean (chimeric protein with N...

Ngày tải lên: 16/02/2014, 09:20

... cholesterol, and proteins, is then stored in the gall bladder. Bile acids excreted into bile undergo intensive enterohepatic circulation. They are reabsorbed in the intestine, taken up again by the liver, ... proteins, promoting binding of the von Hippel–Lindau tumor suppressor protein, which acts as the ubiquitin ligase for HIF-a proteins, leading to the degradation by...

Ngày tải lên: 18/02/2014, 13:20