Báo cáo khoa học: Protein transport in organelles: The Toc complex way of preprotein import pdf

... carrying a cytosolic preprotein. In the pathway, Hsp90 docks to the TPR repeats of Toc6 4 before the preprotein is handed over to Toc3 4 [70]. Certain preproteins, such as the small subunit of Rubisco, ... post-translational import of preproteins into the chloroplast. Three components, the Toc3 4 and Toc1 59 GTPases together with the Toc7 5 channel, form the...

Ngày tải lên: 07/03/2014, 03:20

... through the Toc and Tic complexes. Tic22 may be involved in the stabilization of the Toc/ Tic /preprotein supercomplex. In this model, Tic110 forms the channel protein and also acts in the recruitment ... including the dehydrogenase domain. Spe- cific binding of the FNR is mediated by a unique series of proline/serine-rich repeat motifs located in the C-term...

Ngày tải lên: 07/03/2014, 03:20

... conformation, allowing the transportation of proteins that fold too quickly or tightly for the Sec pathway, or proteins that require the insertion of co-factors in the stroma before transport into the thy- lakoid ... of the twin arginine motif, whereas Hcf106 cross-links less strongly to residues in the hydrophobic core and the early mature protein [32]. Binding...

Ngày tải lên: 07/03/2014, 03:20

... mechanism to increase the complexity of proteomes is the editing of transcripts (both in nuclear and organelle genomes) [4,5]. Dual targeting of proteins does not increase the number of proteins in a cell, ... system used by proteins involved in photosynthesis [36]. The Toc1 59 family in Arabidopsis consists of four proteins: Toc1 59, Toc1 32, Toc1 20 and Toc 9...

Ngày tải lên: 07/03/2014, 03:20

... [4–7]. Toc3 4 GDP interacts with the second G -protein in the Toc complex, Toc1 59, and simultaneously transfers the preprotein to Toc1 59. Toc1 59 action facilitates pre- protein translocation through the ... pTic22 was imported and processed even in the absence of exoge- nous ATP. The yield of pTic22 import in the absence of ATP varied between 20–50%...

Ngày tải lên: 23/03/2014, 07:20

... they are not the sum of their parts – so they can be used for scrubbing. The third is that content words ap- pearing in both feedback sets – for example the wind is blowing” vs. the winds of ... legal straits of the institute. N4 She had a hand in his fully comprehending the quandary. The target set consists of sentences from the corpus containing the target word....

Ngày tải lên: 24/03/2014, 03:20

... aprotinin, leupeptin and pepstatin, twice in kinase buffer, and used for in vitro kinase assays. In vitro kinase assays Recombinant human protein kinase CK2 holoenzyme (0.5 U, corresponding to ... suggesting the absence of CK2 sites N-terminal of position 402 in the catalytic domain and the presence of several phosphorylation sites for CK2 in the C-terminal portion...

Ngày tải lên: 18/02/2014, 16:20

... detergent. Membrane protein integration During the synthesis of a membrane protein, hydro- phobic TM segments move from the aqueous interior of the channel through the lateral gate into the lipid phase. The ... side of the membrane, depending on the amino acid sequence of the protein, which often determines the orientation of subsequent TMs. If the first TM...

Ngày tải lên: 07/03/2014, 06:20

... The structure of the C-terminus of SUMO-1 changes upon protein binding. (C) Sequences of proteins aligned in (B). Residues in capitals are aligned among all modifiers, including the strictly conserved ... composed of APPBP1 (APP binding protein 1) and uba3. The domain structures of Aos1 and APPBP1 resemble the N-termini, and those of uba2 and uba3 resemble the C...

Ngày tải lên: 30/03/2014, 04:20

... riefly, the quantity of protein, present in the respective band of a gel of a certain protein preparation, was determined by comparison with protein standards that were run on the same gel and stained simultaneously ... central unit of the protein translocase [8,10–12]. In addition to the heterotrimeric Sec61p complexes, Hsp70 protein family members of the ER...

Ngày tải lên: 30/03/2014, 15:20