Báo cáo khoa học: Structure of the atrial natriuretic peptide receptor extracellular domain in the unbound and hormone-bound states by single-particle electron microscopy ppt

... Structure of the atrial natriuretic peptide receptor extracellular domain in the unbound and hormone-bound states by single-particle electron microscopy Haruo Ogawa 1 , Yue Qiu 1 , Liming ... single-transmembrane polypeptide, each containing an extracellular ANP- binding domain (ECD), a transmembrane domain, and an intracellular domain consisting...

Ngày tải lên: 07/03/2014, 03:20

... thereby providing a broad binding speci- ficity. In some proteins containing tandem homologous domains, inter -domain interactions fix the relative ori- entation of the domains in a specific multi -domain structure ... (C) Aromatic side chains and one backbone trace of the NMR struc- tures for the N-terminal domain. (D) Aromatic side chains and one backbone trace of t...

Ngày tải lên: 18/02/2014, 14:20

... whether the difference in conformation of the flap domain is related to binding of the third metal ion or the substrate. The ligands for Mn3 in MtSTP were Asp118, Asp191 and Ser160, the last coming ... occurrence of the metal ions correlates with the conformation of the flap subdomain (132–174) and the ‘binding’ of adjacent STP monomers over the active...

Ngày tải lên: 07/03/2014, 09:20

... thus preventing its formation. Binding of FMN and modeling of F 420 H 2 The conformation and binding characteristics of FMN are nearly identical in all of the analyzed structures of F 420 H 2 oxidase ... highlighted in red. The amino acids lining the cavity above the di-iron center are given in blue. The prominent trypto- phan between FMN and the di-iron si...

Ngày tải lên: 07/03/2014, 10:20

... cluster, the position of the ribose and the base of the third nucleotide are located in the vicinity of Thr61 and Arg67–Thr69. In the second cluster, the ribose and the base of the third nucleotide ... bottom of the active site and inserted into the active site of molecule A. The Tyr87 aromatic ring is positioned in the plane that is occupie...

Ngày tải lên: 18/02/2014, 11:20

... roteolytica aminopeptidase (APP) [7,17], they have both been inter- preted as zinc ions, and the positions of the two ions and the geometry of the amino-acids c oordinating them are s imilar in the three ... are showninblueiftheybelongtothecentral b sheetofthecatalyticdomain,greenifthey belong to the four-stranded bsheetofthe dimerization domain, and ligh t blue if th...

Ngày tải lên: 21/02/2014, 03:20

... between the two termini of the inserted Ca 2+ -binding sites in the model structure of the EF-hand of STIM1 is within 15 A ˚ . Accordingly, a total of six glycine linkers is suffi- cient to enable the ... Ca 2+ . Removing the proposed Ca 2+ -coordinating residues in positions 1 and 3 of the EF-hand motif significantly compromised the metal-binding capability o...

Ngày tải lên: 07/03/2014, 00:20

... cou- pling is the key ‘spring’ interlinking the Ca 2+ stores has involved repeating the decoupling experiment of Fig. 1, but inhibiting the oscillators (i.e. the Ca 2+ stores) while leaving the ... Ca 2+ stores? Oscillating Ca 2+ stores can interact by altering the phase of adjacent oscillators through Ca 2+ -induced- Ca 2+ release. Here, coupling by exchange of Ca 2+...

Ngày tải lên: 16/02/2014, 09:20

... double-strand breaks (DSBs) followed by formation of single-stranded DNA by exonuclease digestion. The single-strand portion invades the regions having homologous sequences in the other Correspondence ... cloned into the expression vector pET21a (Novagen) in the NdeIandHindIII sites. The coding region of p48 was cloned into the expression vector pET21a (Novagen) in...

Ngày tải lên: 20/02/2014, 11:20

... on the N-terminal FP, it would be of interest to compare the structure of the internal FP and i ts interaction w ith membrane bilayer, including in particular the structural in uence of proline. ... helical structure was enhanced in association with the model membranes, p articularly in the N -terminal portion of the peptide. According to the infrared stu...

Ngày tải lên: 19/02/2014, 16:20