Báo cáo khoa học: Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design pdf
Báo cáo khoa học: Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design pdf
... http:// www.pymol.org. Stabilization of pyranose oxidase O. Spadiut et al. 792 FEBS Journal 276 (2009) 776–792 ª 2008 The Authors Journal compilation ª 2008 FEBS Improving thermostability and catalytic activity of pyranose 2-oxidase ... FEBS Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational...
Ngày tải lên: 07/03/2014, 03:20
Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... N-terminal and b-he lical domains participate in binding of the polysaccharide, pullulan. Comparison of the active sites of the model of IPU and Dex49A The active site structures of t he model of IPU ... 372, and 373 are the potential catalytic r esidues of I PU. Also, Trp425 of Dex49A, which is the corresponding residue of Trp402 of IPU, is reportedly located in t...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97 doc
... residues of PEX1-ND are indica- ted by closed circles and closed squares, respectively (see text). The conserved residues and class-specific residues of PEX1-ND and VCP- ND are colored yellow and green, ... binding of wild-type and R144A mutant of the N-terminal domain (ND) of valosine-containing protein (VCP)-ND. (A) Two mutants of the conserved basic residues, R135A...
Ngày tải lên: 16/03/2014, 12:20
Báo cáo khoa học: "Search in the Lost Sense of “Query”: Question Formulation in Web Search Queries and its Temporal Change" pptx
... accounted for by Q-queries. We plot this against b’s activity level, measured by the number of queries issued by b in a month. We binned users by their activity levels on the log 2 -scale and compute ... analysis of query logs, e.g., Beitzel et al. (2004) and on long queries, e.g., Bendersky and Croft (2009). Using co-clicking to infer query-query relation- ships was pro...
Ngày tải lên: 17/03/2014, 00:20
Báo cáo khoa học: Multidrug efflux pumps: The structures of prokaryotic ATP-binding cassette transporter efflux pumps and implications for our understanding of eukaryotic P-glycoproteins and homologues ppt
... analyses of cross-linking data and ligand docking, chiefly because of the NBD–TMD reverse domain order, the lack of conserved structural motifs ICD2 and ICD3, and shorter TMD helices and low sequence ... Signature sequence [21]. Also of note, TM helices 4 and 5 are splayed away from one of the TMDs and form the majority of their interhelical contacts with TM helices...
Ngày tải lên: 22/03/2014, 21:20
Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx
... were performed by incubating 2 lLofin vitro 35 S-labeled product with 1 lg of Aos1 ⁄ Uba2, 150 ng of UBC9 and 1 lg of SUMO-1 in sumoylation buffer (20 mm Hepes, pH 7.5, 5 mm MgCl 2 and 2 mm ATP). ... with LRH-1 and to affect LRH-1-mediated gene activation. In addition, the activity of LRH-1 is modulated by post-translational modification. The transcriptional activity of...
Ngày tải lên: 23/03/2014, 06:20
Báo cáo khoa học: B1–Proteases as Molecular Targets of Drug Development B1-001 DPP-IV structure and inhibitor design ppt
... the product was analyzed by SDS-PAGE and activity assay, show- ing a high reduction of the molecular mass and only a loss of the activity of the 20%. CD and 15 N- 1 H-HSQC spectra of this protein fragment ... of a particular class of chymotrypsins within the gut of Abstracts 166 creates a better understanding of the specificity and selectivity of this enzyme an...
Ngày tải lên: 23/03/2014, 15:20
Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt
... consists of the typical signals and spin systems of the indole moiety (triplets of 5-H and 6-H at 7.12 and 7.18 p.p.m., doublets of 4-H and 7-H at 7.43 and 7.75 p.p.m and the singlet of 2-H at ... studies of E. cloacae IDPC (Eur. J. Biochem. 270) 2325 indolepyruvate (triplets of 5-H and 6-H at 7.04 and 7.12 p.p.m., doublets of 4-H and 7-H at 7.39 and 7.42 p...
Ngày tải lên: 08/03/2014, 02:20
Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx
... were eluted from the gel matrix and analyzed by MALDI-TOF ⁄ TOF MS. The PDI–bacitracin was characterized by a combination of in-source decay and CID-TOF ⁄ TOF MS, based on the method described by Kleffmann ... separation of bacitracin by RP- HPLC, with a gradient of 10–90% ACN over 40 min and detection at 252 nm. Bacitracin A and F and bacitracin B and H were purifi...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Ca2+ ⁄ H+ antiporter-like activity of human recombinant Bax inhibitor-1 reconstituted into liposomes pdf
... The amounts of Ca 2+ released by acidic or alkaline pH were expressed as a per- centage of the maximum releasable Ca 2+ using the fluorescence and the radioactivity of 45 Ca 2+ , and were compared ... result of the decrease in pH was assayed by measuring the radioactivity of tritium, as described in Materials and methods (In the figure, the y-axis represents the cpm values...
Ngày tải lên: 29/03/2014, 23:20