Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt

... of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis Andrea Ilg, Peter Beyer and Salim Al-Babili Faculty of Biology, Institute of Biology II, Albert-Ludwigs ... ends of carotenoids. Abbreviations CCD, carotenoid cleavage dioxygenase; GST, glutathione S-transferase; NIST, National Institute of Standards and Techn...

Ngày tải lên: 07/03/2014, 03:20

... Yasuyuki Kawai 4 , Tamiko Nakajima 1 , Chikako Makita 1 , Masako Itoi 1 , Yutaka Tajima 1 , Koichiro Kishi 1 and Toshihiro Yasuda 2 1 Department of Legal Medicine and Medical Genetics, Gunma ... describe the identification of a novel tran- scription starting exon, designated as exon 1a, in human DNASE1 and characterization of the promoter regions of the gene; Sp...

Ngày tải lên: 19/02/2014, 06:20

... primers: gatggatcccatATGGGTG TTGAAGTTGTA annealing around the start codon of the Ppi1 ORF and gactcgagATTAGTCGACTTCTTACGC annealing just before the putative transmembrane domain (capital letter in the ... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero M...

Ngày tải lên: 19/02/2014, 07:20

... 5¢-TAT GGATCCTTAATTAGAAACAAACTGTCTGA TAAAC P2OH 5¢- CTCGAGATTAGAAACAAACTGTCTGATAAACC P1Z 5¢-ATA CCATGGCTGGAGAAGACTTTAAGATC P1ZH 5¢- CATATGACTGGAGACTTTAAGATC P2Z 5¢-TAT GGATCCTCACCACCCAATTTCGGAAAG P2ZH ... Symbols O, Z and H stand for Sno1p, Snz1p and His-tag, respectively. Underlined are the restriction enzyme sites. Primer Sequence P1O 5¢-ATA CCATGGACAAAACCCACAGTACAATG P1OH 5¢- CATATGCAC...

Ngày tải lên: 19/02/2014, 12:20

... 5¢- ATGAGCTCTGCTTTCCTTCCGGGGA-3¢ S1 5¢- ATTGAGCTCACCAGGAGGGCAGGAGG-3¢ mEts-R* 5¢-GACACCTGTCGGTAACCCTTAAAGCC-3¢ mGATA* 5¢- CGGAGTCGCCTAAGGAGAGATGGAGA-3¢ mE-box* 5¢- AGGGTTCCCGATCGGTGTCTGAGAGA-3¢ mEts-F* ... 5¢- GTGGTACCAGTAGCAGCCGCCGCAAG-3¢ K3 5¢- ATGGTACCGGGCTTTAAGGGTTCCCG-3¢ K2 5¢- ATGGTACCGGAAGGAAAGCAGAGCCC-3¢ K1 5¢- ATGGTACCTGGTGATCCAGGGCTTGC-3¢ Sac 5¢- CCGTTCCGAGCTCCGAGCAC-3¢ S3 5¢- ATGAG...

Ngày tải lên: 21/02/2014, 03:20

... Acta 13 64, 222–235. 29 Biagini GA, Viriyavejakul P, O’Neill PM, Bray PG & Ward SA (2006) Functional characterization and target validation of alternative complex I of Plasmodium falci- parum ... ubiq- uinone 10 or 5 mm ferricyanide, the change in absorbance at 340 nm was measured every 10 s for 3 min. A unit of activity was defined as the amount of enzyme that cata- l...

Ngày tải lên: 07/03/2014, 09:20

... tRNA Phe added until a plateau was seen at a threefold molar excess of tRNA Phe over ribosomes (Fig. 7A) . This shows that the ribosomal A- site was saturated with unacylated tRNA [14 ] when maximal ... the autoradiogram in Fig. 3 (lane 14 ). That SF has low synthetase ability in the absence of alcohol and ribosomes is in accordance with an earlier report [3]. The endogenous...

Ngày tải lên: 07/03/2014, 16:20

... H. (19 94) Comparison of the conformation of active and nonactive backbone cyclic analogues of substance P as a tool to elucidate features of the bioactive conformation: NMR and molecular dynamics ... analogues. Most of cis-andtrans-3-substituted prolinoamino acids (P c 3 aa and P t 3 aa, Fig. 1) bearing a side chain of a natural amino acid can be prepared. These prolin...

Ngày tải lên: 08/03/2014, 02:21

... GluV8 The DNA fragment encoding the full-length form of GluV8 (Met1-Ala336) was amplified with a pair of primers (5¢- ATGGGATCCAAAGGTAAATTTTTAAAAGTTAGTT CT-3¢ and 5¢-ATTGGATCCCTGAATATTTATATCAGG TATATTG-3¢) ... fragment carrying the full-length GluSE (Met1-Gln282) was amplified with a pair of prim- ers: 5¢-TATGGATCCAAAAAGAGATTTTTATCTATATG TAC-3¢ and 5¢-ATTGGATCCCTGAATATTTATATCAG...

Ngày tải lên: 16/03/2014, 06:20

... 21 Yanahira S, Suguri T, Yakabe T, Ikeuchi Y, Hanagata G & Deya E (19 92) Formation of oligosaccharides from lactitol by Aspergillus oryzae b-d-galactosidase. Carbo- hydr Res 232, 15 1 15 9. 22 ... that similar mechanisms may have evolved in rhizosphere competent fungi too. In any case, Bga1 is able to act on polymeric b -1, 3- and b -1, 4-galactans. A role for Bga1 in arabinogala...

Ngày tải lên: 16/03/2014, 11:20