Báo cáo khoa học: The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase pptx

... 132–143 ª 2008 The Authors Journal compilation ª 2008 FEBS 143 The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase Rita ... at the value determined from the amino acid sequence (45 320 Da). Measurement of the K d of PLP binding equilibrium PLP binding e...

Ngày tải lên: 07/03/2014, 03:20

... with in the treatment of VP-eUipsis, perhaps at the cost of some degree of the- oretical inelegance. However, this aspect was crucial for handling the gapping data, since the infelicity of gapping ... that reinforces one of the meanings. For instance, gapping is felicitous in passage (3), where con- text supports the symmetric reading, but is infelicitous in p...

Ngày tải lên: 08/03/2014, 07:20

... deletion of Synoviolin causes a change in the intracellular trafficking of NCT. Effect of Synoviolin on the production of Ab NCT is one of the essential cofactors of the c-secretase complex. We therefore ... Supporting Information). We also determined the levels of c-secre- tase-unrelated ER protein (calnexin) and cytoskeleton protein (tubulin) in these cells as th...

Ngày tải lên: 23/03/2014, 04:20

... explained by the proximity of the mutation to the cleavage site, probably resulting in a small interference with the binding of the activator to the FXI zymogen. There are some examples of inherited ... activation loop at the N-terminus of the catalytic domain, are notable. In the zymogen FXI, Ile371 is exposed to the solvent, while in the activated FXI it...

Ngày tải lên: 23/03/2014, 07:20

... errors since they are based on the articles and the distinction between singular and plural. Finally, the tagged instances are stored in a file with their surrounding words. Each line of it con- sists ... 1(a)? modified by one of the words in Table 1(b)? modified by one of the words in Table 1(c)? Figure 1: Framework of the tagging rules Table 1: Words used in the t...

Ngày tải lên: 23/03/2014, 18:20

... understanding of system capa- bilities. Of the remaining 66% of inputs (2166) which were within the functionality of the system, 68% were in grammar. On the within functionality portion of the data, ... sets of scenarios and then freely interacting with each system. For counterbalancing purposes, half of the subjects used the tablet and then the re- mote c...

Ngày tải lên: 20/02/2014, 12:20

... hinder the entrance of acetylcholine to the CAS in the wild- type enzyme, it appears that, in the two mutants (Trp83Ala ⁄ Glu), inhibitors that bind to the PAS did not prevent the entrance of ... mutants: the strong decrease of inhibition of propidium [21] and the increase of remaining activity upon peripheral site saturation by fasciculin [24]. Finally, minor de...

Ngày tải lên: 07/03/2014, 05:20

... fumarate reduction; the use of the quinol-binding site inhibitor HOQNO shows that the inhibitor blocks binding of MQH 2 at the Q P site [27]. Study of the reduction and oxidation of the cytochrome has ... determine binding sites of quinols, inhibition by HOQNO (a structural analogue of menaqui- none), did not allow them to see the binding site of ubiquinol because...

Ngày tải lên: 07/03/2014, 15:20

... accompanied by an increase in the rate of synthesis of Ino, without any need to modify the kinetic parameters or activities of the enzymes involved in the pathway from ATP to Ino. Being aware of the simplifications ... that (a) the main if not unique source of inosine is the intracellular pool of adenine nucleotides, (b) the decrease in ATP and the increase...

Ngày tải lên: 17/03/2014, 10:20

... to peroxidase labelling, density increasing of the Z-line, in particular at the edges of the structure, confirms the immunofluorescent staining. On the other hand, labelling of the Z-band region ... those locating calpain 1 at the midpoint of the I-band, pro- vide reasonable evidence to support the interaction of the protease within the 800 kDa fragment. The...

Ngày tải lên: 23/03/2014, 13:20