Báo cáo khoa học: Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafficking of human acetylcholinesterase and butyrylcholinesterase potx
Báo cáo khoa học: Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafficking of human acetylcholinesterase and butyrylcholinesterase potx
... works Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafficking of human acetylcholinesterase and butyrylcholinesterase Dong Liang 1,2 , ... This indicates a predominant in uence of the catalytic domain on oligomerization. Role of the C-terminal cysteine Mutation of the cy...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx
... protein contains a BRCT domain at the N-terminus and a Pol X domain at the C-terminal region, similar to the overall domain organization of mammalian Pol k. The nucleotide sequence data reported in ... by the UPGMA method, based on the amino acid sequences of the DNA polymerase domains of OsPol k and the other X-family DNA poly- merases (B). (A) OsPol k has a B...
Ngày tải lên: 07/03/2014, 15:20
Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc
... potential of the haem, rate constants o f CO, O 2 and CN b inding to haem, and CD spectra in the Soret region [15–18], suggesting that this residue is critical for maintaining the electronic states of ... exogenous ligand binding access channel a t t he haem distal side and/ or structure in t erms of cyanide binding in the Fe(III) complex. Redox potentials The redox...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Different roles of two c-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii Remodelling of interaction surfaces may enhance microtubule nucleation at low temperature doc
... compensation. Amino acid changes adjacent to the T3 loop and within the T5 loop may in uence the binding affinity and hydrolysis of GTP and ⁄ or the egress of GDP and P i , which may in turn control the assembly ... terminated by telomeres consisting of four repetitions of the motif C 4 A 4 [62]. This stereotypic organi- zation facilitated obtaining the sequenc...
Ngày tải lên: 07/03/2014, 04:20
Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf
... to 30 kDa in size. Subunits of all known GST structures exhibit a two-domain fold, the N-terminal domain and C-terminal domain, including the highly conserved GSH-binding site (G-site) and the more ... differ in only three amino acids: the triad Arg89, Glu117 and Ile172 found in the isoform GSTU1 is replaced by the triad Pro89, Lys117 and Val172 in the GSTU2...
Ngày tải lên: 15/03/2014, 09:20
Báo cáo khoa học: Critical roles of Leu99 and Leu115 at the heme distal side in auto-oxidation and the redox potential of a hemeregulated phosphodiesterase from Escherichia coli pptx
... the ligand binding kinetics of the PAS domain of Ec DOS, as it does in myoglo- bin and hemoglobin. Examination of the effects of these hydrophobic amino acids on the kinetics of O 2 and CO binding ... stable Fe(II)–O 2 complex. CO binding kinetics We next examined the kinetics of CO binding for the mutant proteins of Ec DosH using laser flash photo- lysis (Fig....
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Essential roles of lipoyl domains in the activated function and control of pyruvate dehydrogenase kinases and phosphatase isoform 1 pot
... running in opposite direc- tions. The monomer association involves the ATP-using C-terminal domains interacting near the base of that side of the wedge outside; the N-terminal domains form the ... acetyl-transferase-catalyzing inner domain. E3BP domains: L3, N-terminal lipoyl domain; B, E3 binding domain; I, inner domain which associates with the inner domain of E...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
... (p 0 ), the peptide (0.1–2 lm) was injected into the subphase, and the increment in surface pressure of the LPS film upon intercalation of the peptide dissolved in the subphase was monitored for the ... 0.5 indicates synergy; see Experimental procedures]. To obtain insights into the mode of action underlying the synergistic activity, we investigated the bacteric...
Ngày tải lên: 16/03/2014, 00:20
Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: Blue-light active flavoproteins studied by electron paramagnetic resonance pptx
... performed on the basis of the correlated coupled radical pair model and assuming the fixed orientations of the Trp324 • radical and the flavin radical given by the 3D structure of the protein [86]. The ... are the absence of the signal from the H(5) pro- ton and the larger splittings of the signal pairs arising from H(8a) and H(6) in the anion rad...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatin pot
... discussed in the next section. The active site of all these amine oxidases is nor- mally located in the proximity of the flavin ring of the FAD cofactor and extends along the substrate-binding domain. ... domain organiza- tion in the first line. The third line shows a summary of the identified linear motifs (after filtering processing taking in account the ac...
Ngày tải lên: 16/03/2014, 02:20