Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx

... Authors Journal compilation ª 2009 FEBS Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 Roger Bofill 1, *, Rube ´ n Orihuela 1, *, ... Zn(II) CeMT1 and Cd(II) CeMT1 and 5 : 1 for Zn(II )– and Cd(II) CeMT2, Zn(II )– and Cd(II)–CtCeMT1 and Zn(II )– and Cd...

Ngày tải lên: 07/03/2014, 02:20

... roles in the catalytic process including modulating the rate of electron transfer between MMOR and MMOH [4,5], altering the redox potential of the binuclear iron site [6], optimizing the interaction ... protein B). The active site of the enzyme is located on the a subunit of MMOH and consists of a binuclear iron species located in a hydrophobic pocket approxi...

Ngày tải lên: 17/03/2014, 09:20

... range of concentrations using 15 lgof purified ASNA-1 protein in the presence of 1 m M ATP. The reaction was initiated by the addition of 5 m M MgCl 2 after 10 min incubation of the ASNA-1 protein ... completed within 30 min of recovering the protein preparation. Affinity of ASNA-1 protein for substrates To examine the affinity of the ASNA-1 protein for ATP, the a...

Ngày tải lên: 30/03/2014, 09:20

... cloning the cDNAs of these C. elegans genes and expressing the recombinant proteins in E. coli. The enzymatic and kinetic properties of the proteins were then determined. This is the first time the ... and animal tissues [2 8–3 0]. In addition, the DAAO-enco- ding genes of yeasts [3 1–3 3], fungus [34], fishes [35], and mammals [3 6–4 2], and the DASP...

Ngày tải lên: 30/03/2014, 10:20

... that the most characteristic signatures in the sequences are present in both proteins, tyrosinases and catechol oxidases; these include the six histidine resi- dues involved in the binding of a ... between monophenols and o-diphenols as favored substrate, and also related to the presence or absence of a seventh histidine, adjacent to the sixth histidine invo...

Ngày tải lên: 19/02/2014, 07:20

... mechanism of the deamination reaction and the subsequent metabolism, including the deamination step. The product formed from 4-amino-3-hydroxybenzoic acid by the action of purified 4-amino-3-hydroxybenzoate 2,3-dioxygenase ... of the purified enzyme at 266 nm. The typical protein absorption p eak of 2 80 nm shifts to 265 nm if the protein contains a flavin-type cofac t...

Ngày tải lên: 19/02/2014, 16:20

... but can be inferred from the text. Qi Su et al. (2008) in- vestigates the problem of extracting those kinds of features. There approach utilizes the associa- tion between features and opinion words ... each kind of product. Table 1 shows that our clustering method is effective in collecting product features. The number of features increases rapidly with the number of...

Ngày tải lên: 20/02/2014, 09:20

... hour of binding, regardless of the input titer (Fig. 1B). FPGN-containing peptides facilitate the uptake of adenovirus into CD40-expressing cells To test the ability of CD40 -binding peptides in ... obtained in the absence of peptide, indicating that the FPGN motif is essential. To eliminate the possibility that nonsequence specific amino acid interactions could be...

Ngày tải lên: 20/02/2014, 11:20

... phospha- tidylinositol 3-kinase (PI3K), Akt and glycogen synthase kinase 3 (GSK3). Together, our results support a role of P2X7 receptors in the regulation of neuritogenesis and in the fine control of the ... lm in the presence of Mg 2+ and 68.23 ± 5.714 lm in its absence. No differences were found in the maximal effect or in the Hill coefficients, the...

Ngày tải lên: 07/03/2014, 02:20

... proceed for 10 min, and the fluorescence was then measured at room tem- perature. In uence of interference The in uence of the main constituents of plasma on the determination of 2.00 · 10 )6 molÆL )1 GSH ... solution and showed high binding ability because of the two adjacent bis(b-CD)s. Because of the conformation of the linker of 1, the nitrogen atoms...

Ngày tải lên: 07/03/2014, 05:20