Báo cáo khoa học: Ca2+/calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells docx

... the receptors of insulin and other growth factors. All of these transmembrane receptors contain protein tyrosine kinase activity in their intracellular domain [1]. The signalling pathway of insulin is similar ... that of some other growth factors [2], and the key signalling molecules of insulin are phosphatidylinositide-3 -kinase (PI-3K), phosphotidylino- sitide dependent...

Ngày tải lên: 21/02/2014, 00:20

... compilation ª 2009 FEBS 5325 Ca 2+ /calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells Rosa Go ´ mez-Villafuertes 1 , ... the phosphorylation state of focal adhesion kinase, Akt and glycogen synthase kinase 3, protein kinases that participate in the Ca 2+ ⁄ calmodulin-depen- de...

Ngày tải lên: 07/03/2014, 02:20

... lacked density indicative of binding of LHCII in this region. Indeed, to date, there is no direct structural evidence of how phosphorylated LHCII binds to PSI, although recent cross-linking and antisense ... [6–9]. Conversely, in PSI-favouring light, oxidation of plastoquinone occurs, leading to deactivation of LHCII-specific kinases and dephosphorylation of mobile LHCII by red...

Ngày tải lên: 07/03/2014, 21:20

... an assay of inhibition by HAI-1. Inhibition of TMPRSS13 protease activity by soluble HAI-1 Inhibition of the protease activity of TMPRSS13 was assessed using recombinant soluble forms of HAI-1, HAI-1–NK1 ... analysis with the anti-c-Myc IgG. The intensity of the band of the protease domain was quantified using the protease domain of the protein obtained from E. coli . Enz...

Ngày tải lên: 15/02/2014, 01:20

... complementation of the mutant was observed in the absence of IPTG, likely indicating significant IPTG-independent expression of the protein. Using an antibody raised against the recombinant pro- tein, AtuNDH-2 ... mitochondria. Alignment of representative NDH-2 protein sequences from the four different families (Fig. 2) revealed high conservation in two domains showing most of th...

Ngày tải lên: 19/02/2014, 06:20

... (g) in the presence of 2.2 lM KATII is shown in lines 7 and 8. In lines 9–12, each molecule was tested for inhibition of the transamination reaction in the presence of 10 m M AAD and 2.2 lM KATII, ... form. Uncompetitive inhibition involves the exclusive (or predominant) binding of the inhibitor to the enzyme– substrate complex or to any intermediate downstream of it. I...

Ngày tải lên: 06/03/2014, 00:21

... of translation of the D1 and apoCP47 subunits of the PSII core. The marginal amounts of PSII cores produced in these strains can not associate in LHCII– PSII supercomplexes. These results point ... presence of PSI–LHCI and PSII–LHCII in the WT pattern. In contrast, PSII– LHCII complexes were observed in revertant pmf1 cells. Thus PG deficiency in mf1 and mf2 mostly targ...

Ngày tải lên: 07/03/2014, 15:20

... X 6 remains the same upon engineering of the SBS of XBS, the results obtained in the previous screening experiment directly Table 1. The effect of genetic engineering of the secondary bind- ing site ... importance of several amino acids in the SBS forming hydrogen bonds or hydrophobic stacking interactions with substrates. Weakening of the SBS increased K d values by up to 70-...

Ngày tải lên: 14/02/2014, 19:20

... (DM) domain. Being a noncanonical cysteine-rich DNA- binding motif, this domain has two highly intertwined finger structures that chelate one zinc ion each, and binds to the minor groove of the ... in developing germ cells (Table 2). In terms of inferred function(s), this incidentally indi- cates that paralogs of dmrt1 in fish, although obviously not involved in the first steps...

Ngày tải lên: 14/02/2014, 19:20

... in an inactive form as a monomer; in response to stress, it forms homotrimers that bind to the HSEs in the promoter regions of hsp genes [19]. In addition, an in ammatory cytokine, interleukin-6, ... not Hsp105a, induces the expression of Hsp70 in mammalian cells. Here, we examined the mechanism by which Hsp105b induces the expres- sion of Hsp70. Using a series of deletio...

Ngày tải lên: 18/02/2014, 06:20