Báo cáo khoa học: A novel metallocarboxypeptidase-like enzyme from the marine annelid Sabellastarte magniﬁca – a step into the invertebrate world of proteases pdf

... metallocarboxypeptidase-like enzyme from the marine annelid Sabellastarte magniﬁca – a step into the invertebrate world of proteases Maday Alonso-del-Rivero1, Sebastian A. Trejo3,Mo´nica Rodrı´guez de la ... (Bartholo-mea annulata, Budonosoma granulifera, Cassiopeaxamachana, Condylactys gigantea, Gorgonia ventalina,Lebrunia danae, Palythoa caribaeorum, Physalia phy-salis, Plexaura homomalla, Stichodactyla ... and contains more than 25 different variantsFig. 1. S. magniﬁca Phylum Annelida, Class Polychaeta, SubclassPalpata, Order Canalipalpata, Suborder Sabellida, Family Sabellidae,Genus Sabellastarte...

Báo cáo khoa học: Hydrogen peroxide efﬂux from muscle mitochondria underestimates matrix superoxide production – a correction using glutathione depletion pptx

... situation.Experimental proceduresAnimals and reagentsFemale Wistar rats, aged between 5–8 weeks, were pur-chased from Harlan Laboratories (Livermore, CA, USA)and allowed ad libitum access to chow and water. ... considered as 0%reduction of the nicotinamide cofactors. The ﬂuorescencevalue obtained after the addition of 5 mm malate and 4 lmrotenone was considered as the signal from the fully reducedcofactor ... chain-dependent generation of superoxideanion and its release into the intermembrane space.Biochem J 353, 41 1–4 16.22 Zoccarato F, Cavallini L, Deana R & Alexandre A (1988) Pathways of hydrogen...

Tài liệu Báo cáo khoa học: Anaerobic sulfatase-maturating enzyme – A mechanistic link with glycyl radical-activating enzymes? docx

... GCC GAA TAC GCC TATTAT-3¢ and 5¢- ATA ATA GGC GTA TTC GGC TGCGAG GTT GGC TAC GGC-3¢; for the C27 6A ⁄ C28 2A mutant, 5¢-GGC GTA GCT ACA ATG GCG AAG CATGCC GGA CAT-3¢ and 5¢-ATG TCC GGC ATG CTTCGC ... GGC ATG CTTCGC CAT TGT AGC TAC GCC-3¢; and for the C33 9A ⁄ C34 2A ⁄ C34 8A mutant, 5¢- ACC CAA GCC AAGGAG GCC GAC TTT CTA TTT GCC GCC AAC GGA-3¢ and 5¢-TCC GTT GGC GGC AAA TAG AAA GTCGGC CTC CTT ... insights into the mechanism of thisnew class of radical-AdoMet enzymes.AbbreviationsAdoMet, S-adenosyl-L-methionine; anSME, anaerobic sulfatase-maturating enzyme; anSMEbt, Bacteroides thetaiotaomicron...

Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

... bysite-directed PCR-based mutagenesis [12,30]. Mutagenicantisense oligonucleotides for MCAD  842GﬁC(5¢-GATAAAACCACACCTGTAGTAGCTG-3¢) and MCAD116 5A G(5¢-CCTGTAGAAAGACTAATGAGGGATGCC-3¢) (mutagenic substitutions ... major change in the extinc-tion coefﬁcient of the bound cofactor in the case of the mutants) [15]. The peak absorbance ratios showedﬁrst of all that the wild-type enzyme as puriﬁed here(ratio ... this mutation is not on the catalytic activity of the enzyme. Absorption spectra of the puriﬁed mutantproteinsTo assess whether these mutations affected the afﬁnity of the enzyme for the bound...

Tài liệu Báo cáo khoa học: Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different ﬂanking bases preference pptx

... cold-, drought- and ABA-regulatedgene expression. Plant Mol Biol 24, 70 1–7 13.15 Prabakaran P, An J, Gromiha M, Selvaraj S, UedairaH, Kono H & Sarai A (2001) Thermodynamic databasefor protein-nucleic ... containing a randomized site of 10 bp. The selectedsequences were aligned computationally and the appearance of a base at each position in a motif was presented as a percentage fre-quency of all ... by the primary structures of the divergent DBD and a phylo-genetic classiﬁcation of the ERF family may partlyreﬂect the features in DNA binding of a certain popu-lation of ERF domains. The...

Tài liệu Báo cáo khoa học: "Semantic Information and Derivation Rules for Robust Dialogue Act Detection in a Spoken Dialogue System" pptx

... are collected for this work. The ﬁrst type of data, called A- data, is a travel infor-mation data set harvested from the databases avail-able on the web, e.g., Wikipedia and Google Map. A- data ... and Ω = {A 1, . . . , A q} is the set of DAs. Using the maximum approximation forsummation, (1) can be written as A ∗t= arg max A ΩWP r (A, W|Ut, Ht)≈ arg max A ΩmaxWP r (A, W|Ut, ... determines the user’s dialogue act A ∗tand accordingly decides the current act of the system. The system DA is con-verted to a surface representation by natural lan-Figure 1: Details of the SLU and...

Tài liệu Báo cáo khoa học: "Some Novel Applications of Explanation-Based Learning to Parsing Lexicalized Tree-Adjoining Grammars"" doc

... is a part of the language defined by the grammar. Parsing new sentences amounts to find- ing analogous explanations from the training sen- tences. As a special case of EBL, Samuelsson and ... LTAG parse, the general- ization of the parse is truly immediate in that a gen- eralized parse is obtained by (a) uninstantiating the particular lexical items that anchor the individual ... construct a finite state machine for each of the stored patterns and then traverse the machine using the given test pat- tern. If the machine reaches the final state, then the test pattern matches...

Tài liệu Báo cáo khoa học: "Detecting Novel Compounds: The Role of Distributional Evidence" potx

... 4.1). The task of deciding whether two nouns form a compound or not crucially depends on a variety of factors such as world- knowledge, the situation athand, and the speaker's and hearer's ... contextual features are straightfor-ward to obtain—all we need is a concordance of the candidate compound annotated with parts of speech.Table 6 shows various combinations of numericfeatures, ... Copestake and Lascarides,1997). Each lexical rule takes a pair of nouns of certain semantic type as input, and the output of the rule is a compound noun whose semantic rep-resentation stipulates...

Báo cáo khoa học:Chính sách đối ngoại của Đảng với khu vực Đông Nam Á những năm đầu thế kỷ 21 pot

... Bg trudng Ngoai giao cac nudc ASEAN ldn thfl 34 (AMM 34) diin ra tai Ha Ngi, Thfl tudng Phan Van Khai khang djnh: "Chinh sach ASEAN Id mgt bg phdn cdu thdnh quan trgng trong ... te cua Viet Nam. Su tham gia va ddng gdp cfla Viet Nam doi vdi qua trinh hgp tac, lien kit ASEAN tren cd ITnh vyc da phuang vd song phuang da tao dd thudn lgi cho qud trinh hgi nhap qudc ... rgng quan he song phuong va da phuong vai trd cfla da phuang dl dam bdo viee tdn trgng ddc lap chfl quyln va toan ven lanh thd, khdng can thiep vdo cdng viec ngi bg cfla nhau, binh ddng va cflng...

Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx

... showed an initial lag in catalysis. The duration of lag (in seconds) was calculated from the time axis byextrapolating the linear portion of the progress curve of the coupled assay. The initial rate ... contact region, and its mono-mers are functional [9]. The molecular mass of the yeast enzyme is almostdouble that of the bacterial and human epimerases. A blast search of the yeast epimerase ... vivo and the gradualdisappearance of the initial lag of catalysis, respectively. A BFig. 4. Change of conformation of native epimerase in the pres-ence of (A) 0–0 .6 mM 5¢-UMP and (B) 0–0 .4...

Xem thêm