Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx

... Knowledge of the regions of transferrin required for the binding of gastrin, and of the regions in gastrin required for the interaction with transferrin, is obviously essential to a full understanding of ... saturation, suggest the possible involve- ment of the transferrin gastrin interaction in iron homeostasis. In the present work, the...

Ngày tải lên: 07/03/2014, 02:20

... sig- naling pathways because of the distinctive mode of interaction between the RTK and the ephrin ligand; cells expressing and presenting the ligand interact with neighboring cells expressing transmembrane ... density for most of the substrate pep- tide, including main chain atoms for the )4 through to the +4 position, but partial or no density for the sid...

Ngày tải lên: 16/03/2014, 02:20

... positions 691 and 719 [15]. In order to identify the specific amino acids within the linear interacting epitopes involved in the complex between a-DG and b-DG, alanine scanning of some of the residues ... affinity between recombinant pep- tides spanning the C-terminal domain of a-DG and the b-DG ectodomain by solid-phase binding assays and SPR, demonstrating...

Ngày tải lên: 16/03/2014, 12:20

... role in the epithelium–mesenchymal cell interactions required for normal differentiation and branching of respiratory components of the lung. In addition, MGP mRNA was consistently found in cells from ... (Fig. 6 and results not shown). Although small, this difference in increase in luciferase activity is consistent with the other results obtained, namely the int...

Ngày tải lên: 30/03/2014, 16:20

... decrease in the k cat for Cys. This implies that the hydroxyl group of Tyr108 of EhMGL1 is actively involved in the b-elimination and c-elimination of the C–S bond, but not the b-elimina- tion of the ... 7.0) containing 1mm dithiothreitol and 20 lm PLP, and 3–60 lgÆmL )1 of the recombinant enzymes, at 37 °C for 10 min. The ranges of substrate concentr...

Ngày tải lên: 07/03/2014, 05:20

... the kinase) due to competi- tion of the two enzymes for the target. Indeed, there is experimental evidence for kinases and phosphatases competing for binding to their targets [39]. Assuming the ... a single kinase. We conclude that knowledge concerning phosphory- lation order and the properties of protein–protein interactions is crucial for the elucidation of th...

Ngày tải lên: 07/03/2014, 10:20

... understand the mode of binding and interference with enzyme catalysis. As the inactivation of any enzyme involved in a metabolic chain will render the whole chain inoperat- ive, the inactivation of ... contact between the ubiquinone from the inner membrane and the active site of DHODH [23,27]. Although there are some differences in processing and association...

Ngày tải lên: 07/03/2014, 12:20

... Escherichia coli. The recombinant protein contained an engineered enterokinase cleavage site in the junction between the GST tag and the Rv1771 sequence. Upon 3 h of induction of the pDEST15_Rv1771 ... biosynthesis of colla- gen and other hydroxyproline ⁄ hydroxylysine-containing proteins ⁄ peptides; synthesis of secondary metabolites, hormones and cytokines [1]; o...

Ngày tải lên: 07/03/2014, 12:20

... structural difference between the two isoforms is the presence of an extra protein domain in mSMO l, encoded by the exon VIa [10]. Comparative analysis of the amino acid sequence of the vertebrate members of the ... mammalian SMOs and that they may cooperate in targeting the mSMOl isoform to the nuc- leus. By means of transient expression of the deletion...

Ngày tải lên: 07/03/2014, 17:20

... improved the results of the Jaccard measure by about 15%. 6 We determine this number experimentally as the number of web pages containing the words the and and . 891 matched. On the basis of these, ... evaluation measures. Then we describe the creation of the gold standard. Further, we present the results of the com- parison of the different ranking measures...

Ngày tải lên: 08/03/2014, 02:21