Báo cáo khoa học: Noninvasive imaging of microRNA124a-mediated repression of the chromosome 14 ORF 24 gene during neurogenesis potx

... the target genes of micro- RNA124a (miR124a), and imaged the miR124a-mediated repression of chromosome 14 open reading frame24 (c1 4orf2 4, unknown function) during neurogenesis, using noninvasive ... miR 124 seed sequence of c1 4orf2 4 (Fig. 4C). To quantify the in vitro luciferase activity, represent- ing the miR124a-directed endogenous expression level of the...

Ngày tải lên: 07/03/2014, 02:20

... presence of modifier genes for the EDNRB gene, and the expression of the modifier genes may be influenced by the environmental factors, thereby lead- ing to the phenotypic variability of WS4. One of such modifiers ... representation of the promoters of the EDNRB gene. The EDNRB gene is shown as a line and four arrows show the transcriptional initiation sites of...

Ngày tải lên: 16/03/2014, 13:20

... [13,23,28,30–33]. The genetic evidence for the role of AMPK in the hypothalamus is less clear because the loss of the AMPKa2 subunit in specific hypothalamic cell types resulted in the opposite pheno- type ... entry of fatty acid into mitochon- dria. Thus, the steady-state level of malonyl-CoA in these tissues is determined by the relative activities of ACC and malony...

Ngày tải lên: 14/02/2014, 22:20

... terminal in the sinus gland, as a result of late and progressive isomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonal tract [39,41]. The immunohistochemical ... whereas several somas of the two types were dispersed in the X organ (Fig. 3A), most likely as a result of arte- factual displacement of the cell bodi...

Ngày tải lên: 18/02/2014, 11:20

... 97% inhibition of BHLmL2 and BHAb, respectively. The influence of pH on the activities of the BH enzymes was investigated in the pH range 6–11. The pH optima were determined to be in the basic range (pH ... and BHAb. The temperature dependence of the activities of the BHs was tested in the range 5–56 °C; the apparent optimum temperatures of BHLmL2 were 25–30 °...

Ngày tải lên: 18/02/2014, 14:20

... of the CA D. melanogaster sequences and, on the other hand, the other D. melanogaster sequences more closely related to the CAVI vertebrate isoforms (CAa, CAb and D. melano- gaster-3) or to the ... was observed in the cytoplasm of the cells. The staining appears to be specific of the probe sequence because the staining is clear with the complementary sequence to RpC...

Ngày tải lên: 18/02/2014, 16:20

... approximated by the sum of the probabilities of observing the set intermolecular distances defined in the 3D structure, relative to the likelihood of encountering such distance in the dataset of all protein–RNA ... onto the RNA bases nearest the center of the b-sheet structure of the RRM. We then scored these sequence-variant structures with the distance- depe...

Ngày tải lên: 18/02/2014, 16:20

... interruption of the geometry of the recognition helix for either Antp or the TTF-1 HD, the anomalous amide exchange pattern and the NOE connectivity data of the C-terminal portion of helix III ... inertia. In the context of RSDM, the detailed analysis of the three spectral densities J(0), J(x N ) and J(0.87x H ) allowed us to obtain a rather complete description...

Ngày tải lên: 18/02/2014, 16:20

... autophosphoryla- tion of the Y166F mutant was similar to wild-type 2-Cys Prx. Given the absence of another putative site, we approached the localization of the phosphoryl moi- ety by examining the mass spectra of ... 2-Cys Prx Given the essential role of ATP in the peroxidase activity, we evaluated changes in the structure of 2-Cys Prx brought about by the concer...

Ngày tải lên: 18/02/2014, 17:20

... structure of BmKK4, the first member of subfamily alpha-KTx 17 of scorpion toxins. Biochemistry 43, 1246 9– 1247 6. 29 Yuan DD, Han YH, Wang CG & Chi CW (2007) From the identification of gene organization ... S5. The coelution of the synthetic conomarphin with d-Trp14 (A) or d-Phe13 (B) with the natural conomarphin. Fig. S6. Sequential d aN(i,i +1) connectivities in t...

Ngày tải lên: 18/02/2014, 17:20