Báo cáo khoa học: Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control sidephore and antibiotic biosynthesis in Streptomyces coelicolor A3(2) pdf

... 2009 The Authors Journal compilation ª 2009 FEBS 4827 Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control sidephore and antibiotic biosynthesis in Streptomyces ... of the dmdR1 and adm genes. (A) Organization of the ORFs in the S. coelicolor A3(2) DNA region corresponding to the dmdR1...

Ngày tải lên: 07/03/2014, 02:20

... chapero- nin, but in this case a strong MCAD protein band was seen in the western blots throughout. The proteins were also purified, and the enzyme function and thermostability investigated. The K364R ... disruption of the local bonding and steric hin- drance by the introduced amino acid. Discussion In this paper we have described the protein folding, enzyme functio...

Ngày tải lên: 20/02/2014, 02:21

... has two RPA ssDNA-binding domains, DBD-A and DBD-B, located within the central region, and a third, DBD-C, in the C-terminal region. DBD-A and DBD-B are the most important regions for bind- ing ... (C–E) In vitro pull-down analysis. For each experiment, the indicated pro- teins were incubated together in NaCl ⁄ P i containing 250 mM NaCl at 4 °C for 1 h, and then incub...

Ngày tải lên: 07/03/2014, 21:20

... PP2A. Both the small and middle T antigens encoded by polyomavirus and SV40, and the calmodulin-binding proteins striatin and SG2NA [ 23], bind to the AC c ore of P P2A. However, unlike the B subunits, ... subunit-binding domains in B56a To determine the minimal r egion of B56 that interacted with the PP2A subunit, we utilized an in vitro binding assay using GST-A su...

Ngày tải lên: 08/03/2014, 10:20

... that this genotype originated in the Boer goat and not the Angora goat. The individual CYP17 genes probably originated from two of the subspecies that were used in the breeding of the Boer goat, probably ... P450. Conclusions This investigation clearly identifies, for the first time, two distinctive genes encoding two CYP17 isoforms in both the South African...

Ngày tải lên: 07/03/2014, 06:20

... seedlings. The measured v alues are summarized in Table 2. The total cytokinin content in the grains was approximately threefold lower than in young seedlings. The increase was mainly observed in ... altered cytokinin levels. Seven genes for isopentenyltransferases – cytokinin de novo synthesizing enzymes – were identified in the Arabidopsis genome [2–4]. In addition, t...

Ngày tải lên: 19/02/2014, 16:20

... present in the utterance but in (8) the interpolated is on the outside and is the WH-thing dis- played whereas in (9) the interpolated quantifier is on the inside, and is merely part of the description ... has three bindings: a SUBJECT and two PP-complements. In the two PP bindings, the ranges of the modifier semantic relations ORIG-OF and and DEST...

Ngày tải lên: 20/02/2014, 21:20

... island cor- responds to an independent component of the final sentence. Each individual word in an island specifies a choice point in the search and causes the creation of a state in the ... in the domain, even if they are rough paraphrases. In short, knowledge-based generators are faced with multiple, complex, and interacting lexical con- straints, 3 and the...

Ngày tải lên: 20/02/2014, 22:20

... eliminating interpretations, and reinterpreting others. The linguistic computation constrains plan reasoning by providing the input; the final interpretation must be in the range specified, and ... interpretations. The time has come to integrate the two into a complete system. 4.1. Interaction of the Constraints The plan reasoning phase constrains the results of...

Ngày tải lên: 21/02/2014, 20:20

... suggest the existence of two ligand-binding sites in L 2 (Fig. 6), one of which is the substrate-binding site. Mutually exclusive binding of OAB, NAT and NATA indicates that the binding sites of these ... in the cross-competition between NAT and OAB (Fig. 7C), indicating that NATA and NAT bind at the same or overlapping sites where two mole- cules of OAB also bind,...

Ngày tải lên: 07/03/2014, 05:20