Báo cáo khoa học: The transcription factor ZBP-89 suppresses p16 expression through a histone modification mechanism to affect cell senescence doc

... Center, Dallas, TX, USA). RNA extraction and real-time quantitative PCR Total cellular RNA was extracted from the 293T cells according to the Promega Total RNA Isolation System man- ual. RNA was resuspended ... The Authors Journal compilation ª 2009 FEBS The transcription factor ZBP-89 suppresses p16 expression through a histone modification mechanism to affe...

Ngày tải lên: 07/03/2014, 02:20

... M, Nosaka T et al. (2006) Rac1 and a GTPase- activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factors. J Cell Biol 175, 937–946. 26 Michaelson D, Abidi ... Indeed, although Rac activation is believed to occur primarily at the plasma membrane, BAF60b, as well as BAF6 0a and BAF60c, were found, as expected, entirely localized to the nucl...

Ngày tải lên: 06/03/2014, 09:22

... ReverTra Ace reverse trans- criptase kit (Toyobo Co. Ltd. Osaka, Japan). The RNA and cDNA of each sample were assayed with the gene quant pro dna ⁄ rna calculator to assess concentrations and purification. ... seems to be unavoid- able during normal aging. Therefore, DNA repair in mitochondria is a crucial step to eliminate mtDNA damage and avoid the accumulation of mtDNA muta- t...

Ngày tải lên: 14/03/2014, 23:20

... for amplification of the NOX4 promoter were as follows: 5¢-AACAATCAGTCTAAAAGAGCTGTGTCTT CT-3¢ (forward for GC-box1), 5¢-CTCCAAAATACTGG CAAACATGTGAACAAT-3¢ (reverse for GC-box1), 5¢- TGAGTGGGCAGAGCTGACCCGGTGCGGGT-3¢ ... Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene Masato Katsuyama 1,2 , Hideyo Hirai 3 , Kazumi Iwata 2 , Masakazu Ibi 2 , Kunih...

Ngày tải lên: 29/03/2014, 00:20

... anti-flag WB anti-HA TIAR-flag BOIP-flag HA-hnRNP M RNaseA WB anti-flag WB anti-HA HA TIAR Merged DAPI TIAR-flag BOIP-flag HA-DDX21 RNaseA WB anti-flag WB anti-HA B HA-ASF/SF2 HA-p68 HA-hnRNP M HA-DDX21 Fig. ... necrosis factor- a mRNA, a prototype of ARE-containing mRNAs, in macrophage cell extracts [45]. Similar to TIAR, all three proteins display a major nuclear locali- zation,...

Ngày tải lên: 16/02/2014, 15:20

... estimates for model parameters are subject to a relatively small amount of random variation. The corresponding drawback to such an approach is that its predictions are not validated on actual documents. In ... Unlabeled Data The use of additional anchors has a drawback in terms of being resource-intensive. A feature set may contain many words and phrases, and each of them requir...

Ngày tải lên: 20/02/2014, 16:20

... 5¢-TATACAT ATGGCTTGCAAGTGTGACTGC-3¢; mtl-1_rev: 5¢-AGC TTGTCGACGTTAATGAGCCGCAGCAGTTCCC-3¢; mtl-2_fwd: 5¢-TATACATATGGTCTGCAAGTGTGACT GC-3¢ and mtl-2_rev: 5¢-AGCTTGTCGACGTTAATGA GCAGCCTGAGCACAT-3¢), ... factor in A ˚ 2 , ± 25%, and the R factor is the least-squares residual, which indicates goodness of fit. Strain Cadmium Zinc Scatterer Nr (A ˚ )2d 2 (A ˚ 2 ) R factor Scatterer Nr...

Ngày tải lên: 16/02/2014, 15:20

... protein activates the ras/ mitogen-activated protein kinase pathway and (b) phosphatidylinositol 3-kinase activates the protein kin- ase B/glycogen synthase kinase-3 cascade. Recent data suggest that ... methanol/0.2 M aqueous ammonium acetate (10 : 90; v/v) was added via a T-connector, and the total flow was introduced through a thermospray interface into a Finnigan MAT model TS...

Ngày tải lên: 20/02/2014, 02:21

... fragments of a- lactalbumin display apoptotic activity Serena Tolin 1 , Giorgia De Franceschi 1 , Barbara Spolaore 1 , Erica Frare 1 , Marcella Canton 2 , Patrizia Polverino de Laureto 1 and Angelo ... of the protein. Abbreviations a- LA, a- lactalbumin; BAMLET, bovine a- lactalbumin made lethal to tumor cells; CAC, critical aggregate concentration; HAMLET, human a- lactalbumin made...

Ngày tải lên: 06/03/2014, 09:22

... as a metastatic suppressor. Abbreviations ECM, extracellular matrix; EGF, epidermal growth factor; EGRF, epidermal growth factor receptor; ERK, extracellular signal-related kinase; FACS, fluorescence-activated ... cell attachment. For the invasion assay, the inserts were coated with extracellular matrix gel from Engelbreth–Holm–Swarm mouse sarcoma (Sigma, Santa Clara, CA, USA). On...

Ngày tải lên: 07/03/2014, 00:20