... as a metastatic suppressor.AbbreviationsECM, extracellular matrix; EGF, epidermal growth factor; EGRF, epidermal growth factor receptor; ERK, extracellular signal-related kinase;FACS, fluorescence-activated ... cell attachment. For the invasionassay, the inserts were coated with extracellular matrix gelfrom Engelbreth–Holm–Swarm mouse sarcoma (Sigma,Santa Clara, CA, USA). On the following day, 1 ... Antibody against Aktwas from BioVision, Inc. (CA, USA), and antibody againstphospho-Akt was from Santa Cruz Biotechnology, Inc.(Santa Cruz, CA, USA).Western blot analysis To examine the influence...