Báo cáo khoa học: Mass spectrometric characterization of the covalent modification of the nitrogenase Fe-protein in Azoarcus sp BH72 ppt

... abundant enzymes in the biosphere [1,2]. It consists of the Fe-protein (dinitrogenase reductase, also referred to as NifH), an a 2 dimer of the nifH gene product and of the MoFe-protein (dinitrogenase) ... suggested that nitrogenase Fe-protein was modified by ADP- ribosylation of R102 also in Azoarcus sp. BH72. Further support was obtained by site-directed muta-...

Ngày tải lên: 07/03/2014, 02:20

... thus the presence of the N-terminal methionine may enable detection of Asp1 that would otherwise be invisible in 1 H 15 N- HSQC spectra. Therefore, the presence of the N-termi- nal methionine ... chromatogram obtained with UV absorption at 214 nm, the middle panel is the corresponding total ion-current after infusion into the mass spectrometer, and the bottom panel i...

Ngày tải lên: 18/02/2014, 13:20

... in insulin-secreting b-cells. In order to gain more insight into the physiological role of mir-9 in insulin secretion, we have specifically addressed the link between GSIS and the regulation of ... conditions, there was a dramatic effect on insulin secretion in response to glucose chal- lenge (GSIS). These reports strongly suggest a role for Sirt1 in the regulation of i...

Ngày tải lên: 06/03/2014, 00:21

... K, Hoffmann S, Bauer F, Friedrich U, Kardinal C, Feller SM, Biesinger B & Sticht H (2002) Structural investigation of the binding of a herpesviral protein to the SH3 domain of tyrosine kinase ... CD4 in the endoplasmic reticulum, which is mediated by the cytoplasmic region of the protein [1,2]. This function involves binding and recruitment of the b-transducin repe...

Ngày tải lên: 18/02/2014, 06:20

... Taken together with the inability of P III and P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the bind- ing and uptake of these two isoforms does ... one. In the four pulchellin A-chains, the residues involved in the active site cleft are the same as in abrin and ricin A-chains. This suggests that the catalytic...

Ngày tải lên: 18/02/2014, 16:20

... I (NADH:ubiquinone oxidoreductase), the largest and most complicated of these, is the main point of entry into the respiratory chain for electrons. The mammalian complex I contains nearly 50 ... catalyzes the oxidation of succinate to fumarate in the mitochond- rial matrix as succinate dehydrogenase. Succinate oxi- dation is coupled to reduction of ubiquinone to ubiquino...

Ngày tải lên: 19/02/2014, 02:20

... Da). The mass of the follow- ing theoretical tryptic peptide (SSVTTIINQLYGP- NSGK, m ¼ 1777.927 Da) could not be found, indicating that the C-terminus of the protein is within this sequence. In the ... [16]. The met state is the resting state of the enzyme, and in the absence of substrate about 85–90% of the enzyme is in this state [16]. Both the met an...

Ngày tải lên: 19/02/2014, 06:20

... deletion in the autoinhibitory (NAF) motif failed to interact with PsCBL thus con- firming the authenticity of these proteins and emphasi- zing the importance NAF in the interaction between them (data ... is the first indication showing the phosphorylation of a CBL by its interacting kinase. An understanding of the functional significance of this observation and the e...

Ngày tải lên: 19/02/2014, 07:20

... DNA-binding domain of HSF, whereas clone C2 contained an insert of 2771 nucleotides including introns and an ORF encoding 513 amino acids. By using 5¢-and3¢-RACE, the full-length cDNAs of clones ... clarify the molecular mechanism underlying the stress response in cold-water fish species. Here, we present evidence for existence of two distinct HSF1 isoforms in rainbow trout a...

Ngày tải lên: 19/02/2014, 12:20

... by mass spectrometry and the lability of the sulfogroup in mass spectrometry analysis allows recognition of the modification, and differentiation of sulfotyrosine and phosphotyrosine [38]. Characterization ... the sulfated peptides [21,35]. In the case of AnIB, tyrosine sulfation selectively in uenced the binding to the mammalian a7 but not the a3b2 subtype...

Ngày tải lên: 19/02/2014, 12:20