Báo cáo khoa học: Mass spectrometric characterization of the covalent modification of the nitrogenase Fe-protein in Azoarcus sp BH72 ppt
Báo cáo khoa học: Mass spectrometric characterization of the covalent modification of the nitrogenase Fe-protein in Azoarcus sp. BH72 ppt
... abundant enzymes in the biosphere [1,2]. Itconsists of the Fe-protein (dinitrogenase reductase,also referred to as NifH), an a2dimer of the nifH geneproduct and of the MoFe-protein (dinitrogenase) ... suggestedthat nitrogenase Fe-protein was modified by ADP-ribosylation of R102 also in Azoarcus sp. BH72. Further support was obtained by site-directed muta-genesis of the target arginine of dinitrogenase ... beexplained again by the inability of trypsin to cleaveC-terminal to R102 due to the modification at this resi-due. However, the presence of peak 1625.5 m ⁄ z in the spectrum of the modified Fe-protein...
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... thus the presence of the N-terminal methionine may enable detection of Asp1 that would otherwise be invisible in 1H15N-HSQC spectra. Therefore, the presence of the N-termi-nal methionine ... chromatogram obtained with UV absorption at 214 nm, the middle panel is the corresponding total ion-current after infusion into the mass spectrometer, and the bottom panel is the mass spectrum of the major ... correspondence with the expected composition, indicating that the peptide is of the correct sequence and free of contaminating pro-teins. Five cycles of N-terminal sequencing confirmed the expected...
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Báo cáo khoa học: Sirt1 and mir-9 expression is regulated during glucose-stimulated insulin secretion in pancreatic b-islets ppt
... in insulin-secreting b-cells. In order to gainmore insight into the physiological role of mir-9 in insulin secretion, we have specifically addressed the link between GSIS and the regulation of ... conditions, there was a dramaticeffect on insulin secretion in response to glucose chal-lenge (GSIS). These reports strongly suggest a role forSirt1 in the regulation of insulin secretion. In spite of this, ... is the first report to show that the amount of Sirt1 protein is regulated (post-transcrip-tionally) during GSIS in insulin-secreting b-islets in vivo.Sirt1 is down-regulated in insulin-secreting...
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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc
... K, Hoffmann S, Bauer F, Friedrich U,Kardinal C, Feller SM, Biesinger B & Sticht H (2002)Structural investigation of the binding of a herpesviralprotein to the SH3 domain of tyrosine kinase ... CD4 in the endoplasmic reticulum, which ismediated by the cytoplasmic region of the protein [1,2].This function involves binding and recruitment of the b-transducin repeat-containing protein (b-TrCP) ... constraints. The topology of micelle-associated VpUcyt wasderived from paramagnetic relaxation enhancement of protein nuclear spinsafter the introduction of paramagnetic probes into the interior of...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... Taken together with the inability of P IIIand P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the bind-ing and uptake of these two isoforms does ... one. In the four pulchellin A-chains, the residues involved in the active site cleft are the same as in abrin and ricin A-chains. This suggests that the catalytic reaction is exactly the same. The ... numbering) [26]. Indeed, theseresidues are retained in positions corresponding exactlyto those in the A-chains of ricin and abrin [26]. Over-all, it is therefore unlikely that the A-chains differ...
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Tài liệu Báo cáo khoa học: Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart ppt
... I (NADH:ubiquinone oxidoreductase), the largest and most complicated of these, is the mainpoint of entry into the respiratory chain for electrons. The mammalian complex I contains nearly 50 ... catalyzes the oxidation of succinate to fumarate in the mitochond-rial matrix as succinate dehydrogenase. Succinate oxi-dation is coupled to reduction of ubiquinone toubiquinol at the mitochondrial ... contains four nonidenti-cal subunits: the FAD-binding protein or flavoprotein(Fp, 68 kDa, 622 amino acids), the iron–sulfur protein(Ip, 29 kDa, 252 amino acids), and two membrane-anchor proteins...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... Da). The mass of the follow-ing theoretical tryptic peptide (SSVTTIINQLYGP-NSGK, m ¼ 1777.927 Da) could not be found,indicating that the C-terminus of the protein is withinthis sequence. In the ... [16]. The met state is the resting state of the enzyme, and in the absence of substrate about 85–90% of the enzyme is in this state [16]. Both the met andoxy states of tyrosinases can catalyze the ... coincide with the N-terminus of the intracellular enzyme (Fig. 1). The segments in the N-terminal portion around the copper ligandamino acids of the active site are well conservedbetween the...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... deletion in the autoinhibitory(NAF) motif failed to interact with PsCBL thus con-firming the authenticity of these proteins and emphasi-zing the importance NAF in the interaction betweenthem (data ... is the first indication showing the phosphorylation of aCBL by its interacting kinase. An understanding of the functional significance of this observation and the exact Thr residue that is phosphorylated ... com-plex formation is one of the mechanisms for generatingresponse specificity in the plant cells. In the presentstudy the coordinated transcript data and the in vivoand in vitro interaction data both...
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Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt
... DNA-binding domain of HSF, whereas cloneC2 contained an insert of 2771 nucleotides including intronsand an ORF encoding 513 amino acids.By using 5¢-and3¢-RACE, the full-length cDNAs of clones ... clarify the molecularmechanism underlying the stress response in cold-water fishspecies. Here, we present evidence for existence of twodistinct HSF1 isoforms in rainbow trout and the formation of ... whereas HSP70 is induced in the 35–37 °Crange in zebrafish tissues [6]. However, little is known about the molecular mechanisms underlying the difference in HSPinduction temperatures in fish species....
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Tài liệu Báo cáo khoa học: Physico-chemical characterization and synthesis of neuronally active a-conotoxins docx
... by mass spectrometry and the lability of the sulfogroup in mass spectrometryanalysis allows recognition of the modification, anddifferentiation of sulfotyrosine and phosphotyrosine [38]. Characterization ... the sulfated peptides [21,35]. In the case of AnIB, tyrosine sulfation selectively in uenced the binding to the mammalian a7 but not the a3b2 subtype [21]. The effects of substitution of phosphotyrosine ... purification in applications for synthesis of cholecystokinin-12 and bradykinin containing tyrosinesulfate [57], and could perhaps be applicable to synthesis of a-conopeptides.Synthesis of variants of...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnchuyên đề điện xoay chiều theo dạngđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ