Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt

... half-life of dissociation of 36–105 min [15]. The oxidation of elafin and trappin down-shifts the half-life of there complexes with NE to % 1.3– 1.8 min. On the other hand, the oxidized inhibitor–Pr3 complexes ... the theoretical inhibitor, regardless of the enzyme, the inhibitor and the state of oxidation of the latter, indi- cating that elastin does no...

Ngày tải lên: 19/02/2014, 07:20

... for resistance to intracellular killing, the scavenging of free radicals and the reactivation of degenerated proteins during infection by a family of proteins named ‘thio- redoxins’ is one of the elegant ... [16,26]. Therefore, we assume that in WhiB proteins, one disul- fide bond is formed between the two cysteines of the CXXC motif (CXXXC in the case of WhiB5)...

Ngày tải lên: 18/02/2014, 12:20

... another nonimmune protein, histone H2A, in catfish skin, where a complex cascade of injury-induced proteases is involved in the regulation of the A MP parasin I production [5]. Therefore further ... a band in the range of m olecular m asses expected for the peptide ( 3 kDa). However with the antiserum against intact apoA-I we observed during the first minutes of digest...

Ngày tải lên: 16/03/2014, 18:20

... in the center of the protein to form additional loose ends to be denatured out of the ball of spaghetti. The ESI spectrum of the 200 kDa human comple- ment C4 glycoprotein (of 1714 residues in ... mass increase with increasing time of deamidation (pH 9.6), the best fit of calculated intensity values (squares) was determined (Fig. 9B–E). The correspond- ing mass i...

Ngày tải lên: 18/02/2014, 16:20

... of wild-type eEF2 thereby reducing the rate of protein synthesis and the growth rate of the transformed cells. As shown in Fig. 3B, the doubling rate was only mar- ginally affected by the presence of a nonfunctional eEF2. ... protein (MAP) kinase and mTOR-signal- ling pathways [17]. These signalling pathways activate the eEF2 kinase in response to mitogens and ot...

Ngày tải lên: 07/03/2014, 05:20

... control of the crystal orientation [21]. On the other hand, the b- and c-axes of the nacre tablets are oriented in parallel to the growing front in bivalves, whereas this is not the case in gastropods ... found both in the soluble and insoluble proteins, rendering it diffi- cult to classify according to the standard view on nacre proteins. Database The protein...

Ngày tải lên: 22/03/2014, 16:20

... proteins (Fig. 7). Thus, the addition of a reduc- ing agent appears to be essential for comparing the thermostability of these proteins and the effect of OLPC binding. Par j proteins contain eight ... considered to be the functionally relevant moieties. Therefore, the character of the side chains lining the cavities of Par j 1 and Par j 2 could provide more reveali...

Ngày tải lên: 30/03/2014, 02:20

... signals The N- and C-terminal extensions of Lsm1 p and Lsm8 p were fused to the N- or C-terminus of GFP, respectively, in order to test whether they contain A B Fig. 2. Lsm1 p and Lsm8 p mutant and hybrid ... modification of the Lsm4 p C-terminus in response to stress. Although apparently important for the specific subcellular localization of Lsm co...

Ngày tải lên: 07/03/2014, 02:20

... The small 30S, or S, subunit contains 21 proteins involved in mRNA binding. The 50S, or L, subunit contains 34 proteins, binds to tRNA, and mediates peptidyl trans- fer. Shown in Fig. 4A is the ... mass spectrum of protein [M + 11H] +11 ions at m ⁄ z 490.3 in (B). The observed ions of type c¢ and z¢ Æ define sequences at the N-terminus and C-terminus of the protei...

Ngày tải lên: 18/02/2014, 16:20

... eggs in the one-cell stage to visualize the localization and to mea- sure the mobility of the components in the PGCs. The medaka embryo was peeled off the chorion, and the segment containing the ... diagram of the constructs microinjected into the medaka eggs and transfected into HeLa cells. The olvas, nanos and tudor coding sequences were joined to...

Ngày tải lên: 18/02/2014, 16:20