Báo cáo khoa học: Enzymatic investigation of the Staphylococ

Báo cáo khoa học: Enzymatic investigation of the Staphylococcus aureus type I signal peptidase SpsB – implications for the search for novel antibiotics ppt

... presence of inhibitor, v 0 is the initial velocity in the absence of inhibitor but with (2%) dimethylsulfoxide. The IC 50 value was determined by ﬁtting the percent inhibition versus inhibitor concentration ... as antibiotic targets because of their essentiality, the eas- ier accessibility of the catalytic domain for potential inhibitors as a result of being located on...

Ngày tải lên : 07/03/2014, 01:20

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Tài liệu Báo cáo khoa học: An investigation of the substrate speciﬁcity of the xyloglucanase Cel74A from Hypocrea jecorina pdf

... in the presence and absence of 2 mm inhibitor. The inhibitors were not hydro- lyzed in the time course of these experiments (10 min). Docking experiments Docking of the oligosaccharide XXXG into ... contribution of all four subsites to ligand binding is reﬂected in the higher degree of inhibition of activity on XXXG by cello- tetraose (60% decrease in activity) compared...

Ngày tải lên : 19/02/2014, 05:20

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Báo cáo khoa học: Spectroscopic investigation of the reaction mechanism of CopB-B, the catalytic fragment from an archaeal thermophilic ATP-driven heavy metal transporter potx

... resolved within the manual mixing time. This reversible ligand competition shows that the nucleotide portion of the analogue is responsible for the speciﬁc interaction with the protein. A titration of the ... freezing otherwise invisible intermediates or investigating site-speciﬁc mutants. Kinetic process of ATP hydrolysis Kinetic modelling requires theoretical values for...

Ngày tải lên : 07/03/2014, 00:20

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Báo cáo khoa học: Enzymatic features of the glucose metabolism in tumor cells ppt

... overexpressed in metastatic tumors and speciﬁc inhibition of TKTL1 mRNA can inhibit cell proliferation in several types of cancer cells [22 1–2 24]. However, direct determinations of TK activities in tumors ... mutations have hit a set of key proteins involved in the stabilization of the genome and the regulation of cell proliferation and apoptosis, the transformation in...

Ngày tải lên : 14/03/2014, 23:20

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Báo cáo khoa học: Enzymatic oxidation of NADP+ to its 4-oxo derivative is a side-reaction displayed only by the adrenodoxin reductase type of ferredoxin-NADP+ reductases potx

... Slight alterations in the posi- tioning of the nicotinamide ring in the active site have been observed by comparing the mutant with the wild -type enzyme. The described 0.7 A ˚ shift of the nicotinamide ... emit blue light by ﬂuorescence [8]. We exclude the formation of the species modiﬁed in position 3 of the nicotinamide ring because of the poor reactivity...

Ngày tải lên : 16/03/2014, 11:20

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Báo cáo khoa học: Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated a subunit of the heterotrimeric GTPase Gq potx

... essential for the activity of the mitogenic Pasteurella multocida toxin. Biochemistry 42, 497 1–4 977. 19 Pullinger GD, Sowdhamini R & Lax AJ (2001) Locali- zation of functional domains of the mitogenic ... antibodies enabled us to detect PMT-induced deamidation in situ, indicat- ing them to be powerful probes for characterizing the actions of the toxin. Results An...

Ngày tải lên : 22/03/2014, 16:20

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Báo cáo khoa học: Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum pdf

... derived from the cotton seed plant, is a known inhibitor of dehydrogenases. Inhibition of PfLDH by gossypol derivatives has proved parasiticidal in vitro [2] and the search for speciﬁc inhibitors is underway ... raises the possibility that substrate speciﬁcity is different from the LDHs thus far investigated in detail, both eukaryotic and prokaryotic. To investigate this possi...

Ngày tải lên : 23/03/2014, 09:20

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Báo cáo khoa học: Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki pdf

... improving the manuscript. This study was supported in part by the Promotion of Basic Research Activities for Innovative Bioscience (PROBRAIN), and by KAKENHI, a Grant-in-Aid for Young Scientists ... metabolism enzymes in P. vanderplanki during desiccation The activities of the enzymes involved in trehalose metabolism were investigated during the desiccation of P. vanderpla...

Ngày tải lên : 29/03/2014, 21:20

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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx

... Bradford MMA (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein util- izing the principle of protein-dye binding. Anal Biochem 72, 24 8–2 54. 33 Laemmli UK ... chitinase A. Having established conﬁdence in the validity of the method, we systematically investigated, by using ESI MS, the kinetic properties of chitinase A with pNP- gl...

Ngày tải lên : 20/02/2014, 01:20

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Báo cáo khoa học: Re-evaluation of intramolecular long-range electron transfer between tyrosine and tryptophan in lysozymes Evidence for the participation of other residues doc

... calculated for the initial oxidation of the proteins above pH 4.5 by the inorganic radicals were quantitative. The estimated overall TyrO Æ yields after reaction (2) varied with the protein and could ... of irradiated solu- tion, for which the amino acid composition of peptides was determined, were screened for the ﬂuorescence signal obtained when an excitation wav...

Ngày tải lên : 17/03/2014, 10:20

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