Báo cáo khoa học: In vivo RNA interference in oyster – vasa silencing inhibits germ cell development pptx

... In vivo RNA interference in oyster – vasa silencing inhibits germ cell development Caroline Fabioux 1,2 , Charlotte Corporeau 1,3 , Virgile Quillien 1,3 , Pascal Favrel 1,3 and Arnaud Huvet 1,3 1 ... challenge. To document in vivo gene silencing by RNAi in the oyster, we injected dsRNA targeting the oyster vasa- like gene (Oyvlg). In Drosophila and Ca...

Ngày tải lên: 07/03/2014, 00:20

... opening of voltage-dependent Ca 2+ channels [1]. The resulting in ux of Ca 2+ leads to a rapid rise in intracellular Ca 2+ concentration and trig- gers insulin secretion [2,3]. However, a growing ... prolonged expo- sure of beta cells to tolbutamide results in changes in flux through key enzymes involved in glucose metabolism that, in turn, may impact on glucose-induced insulin secr...

Ngày tải lên: 30/03/2014, 10:20

... labelling of recombinant proteins by means of incorporating a radioactive selenocysteine residue in a C-terminal Sel-tag [18]. Der p 2 carrying the Sel-tag had an intact core sequence and maintained ... Selective inhibition of selenocysteine tRNA maturation and selenoprotein syn- thesis in transgenic mice expressing isopentenyladeno- sine-deficient selenocysteine tRNA. Mol Cell Biol 21,...

Ngày tải lên: 07/03/2014, 21:20

... treatment [ 4–6 ]. Indeed, the epigenetic, genetic and postgenetic cir- cuits restricting cell destiny are becoming increasingly decoded, and their dysfunction is being linked to line- age-dependence ... 5 Dicer Loqs/TRBP Ran-GTP Pri-microRNA Drosha DGCRS Or Proce ssing of pri-microRNAs into pre-microRNA Processing of pre-microRNA into small RNA duplexes Delivery of RISC-microRNA comple...

Ngày tải lên: 16/03/2014, 01:20

... inducing ligand (S-TRAIL) (Fig. 3). However, this study used in vitro transfection of anti-miRNA21 for in vivo cancer therapy, indicating that the in vivo delivery of miRNAs or anti-miRNAs remains ... vitro and in vivo investigations of miRNA production patterns. Second, noninvasive monitoring of miRNAs provides useful information of the possible role of miRNAs involving cellula...

Ngày tải lên: 16/03/2014, 01:20

... a 2.3-fold increase in the flux through HK, an increase in the glycolytic flux (58 nmol EtOHÆmin )1 ) and two- to four-fold increase in the intermediary concentra- tions. The HK reaction still retained ... however, that including reversibility in reactions with high K eq requires the fitting and some times the guessing of kinetic parameters that cannot be easily determined (K m for produ...

Ngày tải lên: 16/03/2014, 05:20

... converted into RNA UDE by simple addition of the same RNA (Fig. 4). The results indicate that the specific confor- mation of RNA UDE is intimately linked to RNA binding, suggesting putative RNA- assisted ... Activation-induced deaminase can deaminate cytosines only in ssDNA, and not in RNA [18]; how- ever, activation-induced deaminase expressed in Sf9 cells is bound to RNA, and...

Ngày tải lên: 22/03/2014, 16:21

... plasmids were constructed by replacing the SIRT Intron5–Exon6–Intron6 alternative splicing context in pZW8 with the Intron 6– Exon7–Intron7 alternative splicing context of SMN1 and SMN2, respectively ... exclusively, most splicing enhancers tend to bind SR proteins, a family of proteins containing one or two RNA- binding domains and a signature RS domain rich in Arg ⁄ Ser dipeptides,...

Ngày tải lên: 28/03/2014, 23:20

... shown that interactions between KCs and endotoxin comprise the initiating event lead- ing to hepatotoxicity in liver injury, including endotox- emia and ischemia ⁄ reperfusion injury [14]. In our studies, ... KCs in drug-metabolizing dysfunction during sepsis FEBS Journal 278 (2011) 230 7–2 317 ª 2011 The Authors Journal compilation ª 2011 FEBS 2317 Role of Kupffer cells in pathogen...

Ngày tải lên: 14/02/2014, 18:20

... structure facilitating protein bind- ing (indirect) and specific amino acid–base contacts (direct). A recent examination of proteinÆnucleic acid structures has shown how minor groove narrowing enhances ... investing time and effort in preparing and analyzing the full-length protein can be extremely rewarding, as shown by the information obtained with the tumor suppressor protein BRCA2 and it...

Ngày tải lên: 14/02/2014, 19:20