Báo cáo khoa học: Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags doc

... high-throughput; methods; mRNA; rapid RT -PCR analysis of unknown SAGE tags (RAST -PCR) ; reverse SAGE (rSAGE); serial analysis of gene expression (SAGE); tag; two-step analysis of unknown SAGE tags (TSAT -PCR) Correspondence Z. ... FEBS REVIEW ARTICLE Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (S...

Ngày tải lên: 07/03/2014, 00:20

... pair of chunks (c x ,c y ): mode A×A : create a shortcut from each word of c x to each word of c y ; mode L×F : create only one shortcut from the last word of c x to the first of c y . The former ... fault of the reordering mod- els, but rather of a too coarse definition of the search space. To have a concrete idea, consider that a small change of the DL from 5 to 6 word...

Ngày tải lên: 19/02/2014, 19:20

... 37 °Cfora maximum of 18 h with shaking to induce the expression of mutant uricase. Then, they were treated at 60 °Cfor1hfor cell lysis and release of the protein. Following the addition of substrate, ... reaction. The utility of this assay was demonstrated in a screen for mutant uricase enzymes derived from the uricase gene of the thermophilic bacterium Bacillus subtilis b...

Ngày tải lên: 23/03/2014, 12:20

... signifi- cant population of conformations in the a region of the /w space. The presence of NOEs between P15 d protons and T14 a proton for peptides 24148 and 24326, and V14 a proton for peptide 23754, ... used for the structural analysis (NMR and CD). CD spectra analysis of monomers and polymers, at a concentration where these peptides were soluble in water, showed similar st...

Ngày tải lên: 08/03/2014, 08:20

... achieved. Determination of the structure of 8K-BLP MALDI-TOF MS analysis of the purified material from the final HPLC yielded a monoisotopic mass of 7409.2 ([M + H] + ) (Fig. 3A). Amino acid sequence analysis of the ... ILPs. Production of 8K-BLP by fat body To identify the tissue that produces 8K-BLP, gene expression of 8K-BLP in various tissues was analyzed by real-tim...

Ngày tải lên: 18/02/2014, 13:20

... transferase from yeast originated from the stocks of H. Sternbach (formerly Max- Planck-Institute, Go ¨ ttingen), while that from E. coli in recombinant form was provided by A. Weiner (University of Washington ... & Cramer F (1978) Amino- acyl-tRNA synthetases from yeast. The generality of chemical proofreading in the prevention of misaminoa- cylation of tRNA. Biochemist...

Ngày tải lên: 18/02/2014, 13:20

... type of nucleotide either at the 5¢ or the 3¢ position. The nucleotides at these two posi- tions always adopt an anti conformation, except for the G at the 3¢ position always found in a syn confor- mation. ... being spread on the b-sheet from b 4 to b 2 in the 5¢ )3¢ direc- tion. More often, the nucleotide at the 5¢ end of the central dinucleotide contacts the loops at the bott...

Ngày tải lên: 19/02/2014, 17:20

... (Gc cyto ) is genetically prepared and substituted for the endogenous Gc, resulting in inter- ruption of signal transduction owing to cytosolic translocation of the Gb subunit from the membrane. ... cells. The formation of diploid cells in medium lacking methionine and lysine depends on the affinities of the protein pairs [12]. To verify our hypothesis that the incorporation of a...

Ngày tải lên: 05/03/2014, 23:20

... [20]: k off ¼ðk offð2Þ Âð½ibuprofen=ðK 2 ½ibuprofenÞ þðk offð3Þ Âð½ibuprofen=ðK 3 ½ibuprofenÞÞ þ k þ off ð7Þ where k off(2) and k off(3) indicate values of k off occurring at K 2 < [ibuprofen] ... for ibu- profen binding to tHSA–heme-Fe(II)-NO (K 2 and K 3 ) were obtained from the dependence of k off on the free ibuprofen concentration. Values of K 2 and K 3 were determined...

Ngày tải lên: 06/03/2014, 01:20

... primer, 5¢-AGGGCAGATGTCCAGAGCAT -3¢; reverse primer, 5¢-GTCTTCATCAGCCCAGCAGTT -3¢. PCRs involved an initial denaturation step at 94 °C for 5 min, followed by 40 cycles of amplification (94 °C for 30 s, 60 °C for 30 s ... concomitant expression of GFP allowed transfected cells to be identified by fluorescence microscopy. RT -PCR and quantitative real-time PCR Total RNA was extrac...

Ngày tải lên: 07/03/2014, 02:20