Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot

... affecting the SWI ⁄ SNF complex caused reduced activation of these genes within the same time window. The activator-binding domains in the Swi1 and Snf5 subunits of the SWI ⁄ SNF complex are essential ... activation domains and the activator-binding domains of Swi1 and Snf5 of the yeast SWI ⁄ SNF chromatin remodeling com...

Ngày tải lên: 07/03/2014, 00:20

... tris-tricine gels depending upon whether they are pre-incubated in dithithreitol, or not (Fig. 1H). Protease inhibition activity of eppin and its domains Eppin and the two domains were compared for their ability ... were exposed to the two domains of eppin in solution together, survival again dropped to approximately 20%. This suggests that the individual domain...

Ngày tải lên: 23/03/2014, 07:20

... the correct positioning of the SAP motifs in relation to the rest of the protein as well as for the stability of the protein. The truncated Ydc2 mutant proteins localize to the mitochondria in S. ... triple-helix motif was required to hold the junction in a square-planar conformation, it was interesting to test whether RuvA binding to the junction...

Ngày tải lên: 20/02/2014, 11:20

... expression of the insulin-like growth factor-I-binding protein-1 (IGFBP-1) phosphoisoform in pre-eclamptic Wharton’s jelly and its role in the regulation of collagen biosynthesis. Clin Chem Lab ... Cellular actions of the insulin growth factor binding proteins. Endocr Rev 23, 824–854. 3 Baxter RC (2000) Insulin-like growth factor (IGF-) binding proteins: interactions with IG...

Ngày tải lên: 07/03/2014, 00:20

... little correspondence in the amino acids that are involved in the recognition of the functional groups of the ligands. Moreover, in GGT the a-carboxyl group of the gluta- mate protrudes into the groove, whereas in ... present in most other GGTs at the C-terminal region of the L-subunit. In E. coli GGT, upon autocatalytic cleavage of the peptide bond...

Ngày tải lên: 22/03/2014, 21:20

... suppres- sion of the cysteine. Thus, it appears that the effect of a C-terminal cysteine on the trafficking of cholinesterase in the secretory pathway largely depends on the nature of the preceding catalytic ... subunits [37]. This indicates a predominant in uence of the catalytic domain on oligomerization. Role of the C-terminal cysteine Mutation of the cyst...

Ngày tải lên: 07/03/2014, 03:20

... orientations in the membrane; the N terminus of the N-terminal domain is extracellular, while the N terminus of the C-terminal domain is intracellular. In the [st326]2HCT family, TMS I is not part of the ... as observed for the percentage of hits between the N-terminal domains or the C-terminal domains alone. The results strongly suggest that the N- a...

Ngày tải lên: 07/03/2014, 17:20

... better the contribution of the b¢ binding site to PDI activity, the inhibitory in uence on the refolding of RNase A caused by the peptides binding b¢ was examined. In the assay, incubation of unfolded ... domain increased the K m to 30 lm, and loss of an additional cys- teine in the a domain resulted in an increase of the K m to 50 lm [25,27]. On the...

Ngày tải lên: 16/03/2014, 04:20

... lycophosphatidylinositol-linked NgR, via its leucine-rich r epeat containing do main. The binding of Nogo-66 to N gR is competitively inhibited by a peptide consisting of the N-terminal 40 residues of Nogo-66, named ... be induced to form a helical structure via interaction w ith Zn 2+ . Furthermore, the d etermination of the Nogo-40 solution structure offers a starting...

Ngày tải lên: 16/03/2014, 16:20

... [15–18]. These actin-binding activities are conserved in the EVH2 domain [19,20]. On the other hand, the EVH1 domain binds to proteins containing the consensus sequence D ⁄ E FPPPPXD ⁄ E [21–23]. The ... clarify the contributions of these proteins in the HRR pathway. During the cytoplasmic actin -remodeling process, the ENA ⁄ VASP family proteins bind...

Ngày tải lên: 23/03/2014, 04:20