Báo cáo khoa học: Ion-binding properties of Calnuc, Ca2+ versus Mg2+ – Calnuc adopts additional and unusual Ca2+-binding sites upon interaction with G-protein pdf

... Ca 2+ versus Mg 2+ – Calnuc adopts additional and unusual Ca 2+ -binding sites upon interaction with G-protein Madhavi Kanuru*, Jebakumar J. Samuel*, Lavanya M. Balivada* and Gopala K. Aradhyam Department of ... Ca 2+ -binding sites is known to generate the J-band (61 0–6 50 nm) and ⁄ or the c-band (48 0–5 10 nm). Upon binding to Calnuc, the dye dis...

Ngày tải lên: 07/03/2014, 00:20

... An overview of the new, refined models. Models A2bB3–bc (panels A and F), A2bB3-pec (panels B and G), A2bB3-ec (panels C and H), A2bB3-eotc (panels D and I) and A2bB3-eots (panels E and J), are ... prediction of the O-glycosylation sites (see the Materials and methods), S218 and T615 were selected in place of T259 and T619, respectively, for two O-linked carbohydrates...

Ngày tải lên: 18/02/2014, 04:20

... nature of interactions between K-Ras and cellular membranes, and indicate that subcellular distribution of K-Ras is driven by electrostatic interaction of the polybasic region of the protein with ... dis- ruption of the electrical properties of the outer and inner leaflets of plasma membrane and ionic gradients through it. Removal of sialic acid from the outer pla...

Ngày tải lên: 19/02/2014, 00:20

... Therefore, interactions of cystatin F with several of its potential targets, cathepsins F, K, V, S, H, X and C, were studied in vitro. Cystatin F tightly inhibited cathepsins F, K and V with K i values ranging ... buffer pH 6.0 with different concentrations of dithiotreitol. After dilution, cystatin F was equilibrated with a twofold molar excess of papain. The residual act...

Ngày tải lên: 19/02/2014, 17:20

... parameters of chitinase A with various substrates. The hydrolysis of chitooligosaccharides and colloidal chitin at sub- strate concentrations of 0–2 m M was carried out with 75 ng of native chitinase ... popu- lation of reaction intermediates. The monomer, GlcNAc, only appeared after a lag time of  30 min, and the larger oligomers – (GlcNAc) 4 and (GlcNAc) 6 –...

Ngày tải lên: 20/02/2014, 01:20

... determinants of v-myb. N- and C-terminal deletions remove several sites of protein modification, including an N-terminal CK2 phosphoryla- tion site (S11 and S12) [10], and a putative MAPK-site (S528) [1 1–1 3] ... Immunoprecipitation of c-Myb with polyclonal anti-(c-Myb) Ig, and detection with monoclonal anti(-c-Myb) Ig revealed the main c-Myb band at 75 kDa and several...

Ngày tải lên: 21/02/2014, 00:20

... Rev 198, 11 6–1 26. 12 Sugumaran M (2002) Comparative biochemistry of eumelanogenesis and the protective roles of phenoloxi- dase and melanin in insects. Pigment Cell Res 15, 2–9 . 13 Land E, Ramsden ... v 0 (M·min –1 ) × 10 –4 v 0 (M·min –1 ) × 10 –4 Fig. 7. Inhibition of activated hemocyanin. The inhibition of SDS-activated hemocyanin (0.42 mgÆmL )1 ) by...

Ngày tải lên: 07/03/2014, 05:20

... fragments 4 0–5 6 and 6 0–6 3 are common to both chromatograms, fragments 4 0–5 7 and 5 8–6 3 arose only from the monomeric form (Fig. 3B). The tripeptide 5 7–5 9 complementing frag- ments 4 0–5 6 and 6 0–6 3 which ... the monomeric form and 45% in the trimeric form, and b-structure is absent from both forms. The CD spectra of the 1–7 0 and 7 0–9 0 regions of...

Ngày tải lên: 07/03/2014, 14:20

... forma- tion of dimers with an apparent molecular mass of 42 kDa. At pH 6.0, Hsp20 and its S16D mutant dissociated, and were eluted in the form of two peaks with apparent molecular mass values of 4 5–5 0 and ... of subunits and formation of a mixed oligomer with an apparent molecular mass of 95 kDa (Fig. 9B). Thus, Hsp20 and Hsp27 may form two types of hetero-...

Ngày tải lên: 07/03/2014, 15:20

... role of ARM-1 in lymphocyte adhesion was studied by comparing adhesion properties of ARM-1-nonexpressing cells before and after transfec- tion with ARM-1 cDNA. The assays were carried out with ... Glycosylation in the nucleus and cytoplasm. Annu Rev Biochem 58, 84 1–8 74. 25 Hart GW (1997) Dynamic O-linked glycosylation of nuclear and cytoskeletal proteins. Annu Rev Bioche...

Ngày tải lên: 07/03/2014, 17:20