Báo cáo khoa học: Differential recognition of heat shock elements by members of the heat shock transcription factor family ppt

... (2009) 1962–1974 ª 2009 The Authors Journal compilation ª 2009 FEBS Differential recognition of heat shock elements by members of the heat shock transcription factor family Noritaka Yamamoto1, ... Roles of the heat shock transcription factors in regulation of the heat shock response and beyond. FASEB J 15, 1118–1131.2 Voellmy R (2004) On mechanisms that control heat shock transcription factor ... shock transcription factor; HR, hydrophobicrepeat; HSE, heat shock element; HSF, heat shock transcription factor; HSP, heat shock protein; mHSF, mouse heat shock transcription factor; SD, standard...

Báo cáo khoa học: Heavy metal ions inhibit molybdoenzyme activity by binding to the dithiolene moiety of molybdopterin in Escherichia coli pdf

... form of the molybdenum cofactor. The ability of various metals tobind tightly to sulfur-rich sites raised the question of whether other metalions could be inserted in place of molybdenum at the ... requiring the catalytic activity of the MoeA protein. Although the synthesis of the dioxoMoco found in hSO seems to be more susceptible tononspeciﬁc metal insertion, the biosynthesis of the bis-MGD ... generates the molybdenum cofactor, which can be inserteddirectly into molybdoenzymes binding the molybdopterin form of the molybdenum cofactor, or is further modiﬁed in bacteria to form the dinu-cleotide...

Báo cáo khoa học: Caspase-2 is resistant to inhibition by inhibitor of apoptosis proteins (IAPs) and can activate caspase-7 pot

... A proportion of caspase-2D1)149was cleavedto remove the small subunit, thereby permitting detec-tion of the dissociated large subunit. The activities of these different forms of caspase-2were ... caspase-3 and caspase-7), which thencatalyse the cleavage of numerous cellular substrates[4]. Despite being the second identiﬁed member of the caspase family, the function of caspase-2 (Nedd-2 ⁄Ich-1) ... as an upstream caspase, caspase-2 could provoke the activation of caspase-7. Caspase-2 was not subject to inhibition by members of the IAP family of apoptosis inhibitors.AbbreviationsAFC, 7-amino-4-triﬂuoromethyl...

Tài liệu Báo cáo khoa học: Gas6 and protein S Vitamin K-dependent ligands for the Axl receptor tyrosine kinase subfamily pptx

... [2]. One of these is the subfamilycomprising Axl, Sky and Mer (as we shall refer tothem hereafter), also referred to as the TAM family (Tyro3, Axl and Mer). This RTK subfamily is deﬁned by a combination ... and Mer. Furthermore, ahallmark of the Gas6–Axl system is the unique ability of Gas6 and pro-tein S to tether their non receptor-binding regions to the negatively chargedmembranes of apoptotic ... revealed the necessity of the ﬁrst LGdomain in the Gas6 SHBG region for Axl binding[49]. More recently, the publication of the crystalstructure of a minimal Gas6–Axl complex has provi-ded, for the...

Báo cáo khóa học: Differential carbohydrate epitope recognition of globotriaosyl ceramide by verotoxins and a monoclonal antibody pdf

... deoxyGb3bindingBased on these results, we suspected that modiﬁcation of the VT1B by FITC-conjugation might have subtly altered the ability of VT1B to bind Gb3. We therefore compared the binding of FITC-labelled ... gate at800 Da. The mass spectra were externally calibrated with the molecular mass of a mixture of standard peptides.ResultsComparison of ligand/Gb3binding by RELISA The binding of VT1, VT2, ... tre-ated without FITC–VT1B. The sensitivity of the immuno-peroxidase detection allowed for the use of 1 lgÆmL)1FITC–VT1B instead of the 5 lgÆmL)1used for visualization by ﬂuorescence.Immunostaining...

Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

... (Fig. 4B). The ﬁnding of these differential functions is in linewith the dogma that the proteome gains functionalcomplexity by encoding multiple isoforms of a givenprotein, with these isoforms ... alternative isoforms of members of the p53 ⁄ p63 ⁄ p73 [23] and the Bcl2 families [24].On the molecular level, we show that the uniquePRD on Mxi1-SRa contributes to the differential func-tions of Mxi1-SRa ... region of the ODC promoter bearstwo E-box elements that are repressed by Mxi1 butactivated by Myc [17]. As such, the similar effects of the two Mxi1 isoforms on this promoter are in the linewith...

Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherﬁsh Misgurnus fossilis Functional consequences for the enzyme ppt

... the face of temperatureacclimation. Therefore, we think that the describedmechanism maintains either (a) the kinetic⁄ thermody-namic properties of this metabolic node by ‘dilution’ of the major ... in the sub-unit contact area of the tetramer, whereas the remaining two mismatches sur-round the contact area; this is expected to manifest in the kinetic andthermodynamic properties of the ... Assemblage of the clones yield the full-length cDNA sequences of at least twodistinct LDH-A isoforms, which differ substantially in the coding sequence and length of the 3¢-UTR. Therefore, iso-form-speciﬁc...

Báo cáo khoa học: Differential membrane compartmentalization of Ret by PTB-adaptor engagement pdf

... that the majority of Ret partitioned in the DRM fraction.This was in contrast to the 2aRetY1062Fmutant, where the majority of Ret was found in the SUP fraction(Fig. 1A). To test whether ... performed by placing electrodes along the rostral–caudal axis of the neural tube. The egg wasthen closed and placed in an incubator until the embryos had grown to stage 24 (E4.5). At this stage, the ... affected by activation within or outside DRMfractions [23]. To examine the role of the PTB adaptorsin the activation of the MAPK and Akt pathways,spinal cords electroporated with Ret together...

Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

... result of the residue’s location at the edge of the dimer–dimer interface. The maximum oligomer sizeobtained with p26 R114A was  500 kDa, closer to the mass of the WT oligomer than the other ... WHSP16.9, wheat Hsp16.9,1GME. sHSP domains based on the sequence of p26 are indicated above the alignment, secondary structure elements based on the sequence of wheat Hsp16.9are below the alignment, ... were excluded. Application of procheck [76] revealed that the stereochemical quality of the model was reliable, with 84% of the residues in the most favored regions of the tetramer model and none...

Báo cáo khoa học: Differential interactions of decorin and decorin mutants with type I and type VI collagens pptx

... Models of the potential interaction of decorin with type I a ndtype VI co llagens. Decorin is shown by the grey arrow, the tip of the arrow represents the C-terminus; the dashed line indicates the ... ﬁbrilforming collagens are traversed speciﬁcally near their ÔdÕbands, within the gap region of the collagen ﬁbrils, by the ﬁlamentous beaded structures of the type VI collagen-containing network [4,5].Collagen ... [19]. During immobilization, a ﬂowrate of 10 lLÆmin)1 of HBS was maintained. The surface of the chip was activated by injecting a mixture of equalvolumes of 0.2MN-ethyl-N¢-(3-dimethylaminopropyl)-carbodiimide...

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