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Báo cáo khoa học: Assembly of nuclear DNA-encoded subunits into mitochondrial complex IV, and their preferential integration into supercomplex forms in patient mitochondria doc

Báo cáo khoa học: Assembly of nuclear DNA-encoded subunits into mitochondrial complex IV, and their preferential integration into supercomplex forms in patient mitochondria doc

Báo cáo khoa học: Assembly of nuclear DNA-encoded subunits into mitochondrial complex IV, and their preferential integration into supercomplex forms in patient mitochondria doc

... compilation ª 2009 FEBS 6713 Assembly of nuclear DNA-encoded subunits into mitochondrial complex IV, and their preferential integration into supercomplex forms in patient mitochondria Michael Lazarou1, ... imported complex IV subunits can integrate into complex IV and its supercomplexes by associating withpre-existing subunits and integrating into intermediatecomplexes. A novel late-stage intermediate ... into the maturing complex IV. Integration of nDNA-encoded subunits into supercomplexes in patient mitochondria Using digitonin solubilization of mitochondria to visu-alize supercomplexes, it...
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Báo cáo khóa học: Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 ppt

Báo cáo khóa học: Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 ppt

... the ER extract of PSG cells of a normal-level fibroin-producing silkwormTable 2. Determination of molar ratios of H-chain, L-chain, and fhx/P25 in the fibroin secreted into cocoons of a normal breed ... glycoprotein [28,29], in uenzavirus hemagglutinin [30,31], and IgM [32] in ER, a furtherrole of N-linked oligosacharide chains of fhx/P25 in theprocess of assembly of the elementary unit in ER ... unit of fibroin [1], which consists of six sets of heavy chain (H-chain; 350-kDa fibrous protein)–light chain(L-chain; 26 kDa) disulfide-linked heterodimer and onemolecule of a glycoprotein, fibrohexamerin...
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Báo cáo khoa học: Suppression of nuclear factor-jB activity in macrophages by chylomicron remnants: modulation by the fatty acid composition of the particles pot

Báo cáo khoa học: Suppression of nuclear factor-jB activity in macrophages by chylomicron remnants: modulation by the fatty acid composition of the particles pot

... acid and docosahexaenoicacid, which are found in fish oil, downregulate LPS-induced NF-jB DNA binding and p65–NF-jBexpression, and increase IjBa expression and doco-sahexaenoic acid and ⁄ ... (Fig. 2).Effects of CRLPs on cytokine and chemokinesecretion and mRNA expression in macrophagesWe initially examined the effects of CRLPs on therelease of TNFa, IL-6, IL-1b and MCP-1, which ... mL) and incu-bations continued in fetal bovine serum-free culture mediumfor 24 h in the presence of ApoA-I ⁄ phosphatidylcholine(100 lgÆmL)1). At the times indicated in the text, aliquots of the...
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Báo cáo khoa học: Control of nuclear receptor function by local chromatin structure doc

Báo cáo khoa học: Control of nuclear receptor function by local chromatin structure doc

... pS2 gene. In the initial experiments the increase of acetylation of histone H3 and H4 at ER-regulatedcathepsin D and pS2 promoters was observed within10 min of estrogen treatment, peaking at 1 ... (5-aza-2¢-deoxycyti-dine, 5Aza-dC) results in a loss of methylation, loss of BRM and MeCP2 binding and reactivation of tran-scription [174]. In cancer cells, changes in DNA meth-ylation promoted by SWI ⁄ SNF complexes ... corepressors and chromatin remodeling in the control of transcription.J Mol Endocrinol 23, 255–275.18 Hsiao PW, Deroo BJ & Archer TK (2002) Chromatinremodeling and tissue-selective responses of nuclear hormone...
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Báo cáo khoa học: Inhibitor of nuclear factor-kappaB alpha derepresses hypoxia-inducible factor-1 during moderate hypoxia by sequestering factor inhibiting hypoxia-inducible factor from hypoxia-inducible factor 1a ppt

Báo cáo khoa học: Inhibitor of nuclear factor-kappaB alpha derepresses hypoxia-inducible factor-1 during moderate hypoxia by sequestering factor inhibiting hypoxia-inducible factor from hypoxia-inducible factor 1a ppt

... FIH bindingTo understand the interplay between HIF-1a, FIH, and IjBa, we coexpressed these proteins in HEK293cells and examined protein interactions by performingimmunoprecipitation and immunoblotting ... interactions with ASB4 and Notch-1. By using yeasttwo-hybrid screening, Ratcliffe et al. identified twoARD-containing proteins, p105 and uveal autoantigenwith coiled-coil domains and ankyrin ... protein 4 (ASB4) and Notch-1 ⁄ 2 were tar-geted and hydroxylated by FIH [7,8]. Ankyrin repeatdomains (ARDs) in these proteins have been found tobe FIH-binding sites. Moreover, Asn246 of ASB4 and Asn1945...
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Báo cáo khoa học: Assembly of collagen types II, IX and XI into nascent hetero-fibrils by a rat chondrocyte cell line ppt

Báo cáo khoa học: Assembly of collagen types II, IX and XI into nascent hetero-fibrils by a rat chondrocyte cell line ppt

... using pepsin. Pepsin cleaves in the telopeptide domains of type II collagen and in thenoncollagenous domains of the minor collagens, type XI and IX, but leaves their triple helical domains intact. ... to interfere with the interactions needed to formcross-links and mature them into trivalent pyridinolineresidues. In addition, using antibodies that recognize specificcross-linking domains, ... showing thin filaments in an otherwise amorphousbackground (Fig. 1). In summary, the findings support the integration and intermolecular cross-linking of type II N-procollagen withtypes IX and...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... have indi-cated that the CagAPY-induced cell elongation pheno-type also involves tyrosine dephosphorylation of cortactin, vinculin and ezrin, which are three well-known actin-binding proteins ... the amount of intracellu-lar CagAPY. Cortactin, ezrin and vinculin are all Srcsubstrates, and Src inactivation causes dephosphoryla-tion of these proteins and is crucial for triggering cellelongation ... of bacteria gain access to integrins and inject CagA. Thebasal injection model of CagA can also explain whyH. pylori does not cause more gastric damage in infected individuals and may only inject...
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Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... pheromonesto elicit a mating response in cells of the oppositemating type. Cdc42 is involved in pheromone signal-ling that eventually results in G1arrest and the for-mation of a mating projection ... formation of a matingprojection [28]. Furthermore, homologues of oxysterol-binding proteins, a family of proteins that regulatethe synthesis and transport of sterols, were found toparticipate in ... C-terminal halves of ubiquitin (N-Ubi and C-Ubi) alone do not assemble. If a protein ‘X’,fused to N-Ubi, binds to the PAKs Ste20p or Cla4p, linked to C-Ubi,both halves of ubiquitin are brought into...
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Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... sulfo-bromophthalein to rat and human ligandins: characteri-zation of a binding-site peptide. Biochim Biophys Acta955, 296–300.52 Gregus Z & Klaassen CD (1982) Role of ligandin as abinding protein and ... anthocyanin sequestration, is a flavonoid-binding protein. Plant Physiol 123, 1561–1570.24 Grotewold E (2004) The challenges of moving chemicalswithin and out of cells: insights into the transport of plant ... bilirubin, biliverdin, section B) and in Table 2 (Kd of the complexes of bilitranslocase with bilirubin, nicotinic acid and cyanidin3-glucoside, sections C and D).A Michaelis–Menten constants of...
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Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx

Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx

... Analysis of two CBP(cAMP-response-element-binding protein-bindingprotein) interacting sites in GRIP1 (glucocorticoidreceptor-interacting protein), and their importance forthe function of GRIP1. ... conserved AF-2 domain of NRs [22]. The C-terminal region of the p160 co-activa-tor can interact with the AF-1 domain of some NRs and enhance their AF-1 activities in the absence of lig-ands [23–25].Recent ... by acety-lating or methylating histones or other proteinsinvolved in the transcription machinery [5,10,19–21].Two separate domains of p160 co-activators can bindto AF-1 and AF-2 of NRs. The...
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