Báo cáo khoa học: Cholesterol oxidase: biochemistry and structural features ppt

... ª 2009 FEBS 6843 MINIREVIEW Cholesterol oxidase: biochemistry and structural features Alice Vrielink 1 and Sandro Ghisla 2,3 1 School of Biomedical, Biomolecular and Chemical Sciences, University ... SChOx and b) BsChOx have been included in red (alpha helices) and blue (beta strands). Biochemistry of cholesterol oxidase A. Vrielink and S. Ghisla 6828 FEBS Journal 27...

Ngày tải lên: 07/03/2014, 00:20

... Streptomyces cholesterol oxidase by random mutagenesis and a structural interpretation. Protein Eng 10, 231–235. 59 Lim L, Molla G, Guinn N, Ghisla S, Pollegioni L & Vrielink A (2006) Structural and ... (1985) Amperometric assay of total and free cholesterols in serum by the combined use of immobilized cholesterol esterase and cholesterol oxidase reactors and peroxidas...

Ngày tải lên: 07/03/2014, 00:20

... determination of cholesterol oxidase from Streptomyces and structural characterization of key active site mutants. Biochemistry 38, 4277–4286. J. Kreit and N. S. Sampson Cholesterol oxidase and membranes FEBS ... reaction catalyzed by cholesterol oxidase. Biochemistry 36, 6133–6140. 37 Slotte JP (1992) Cholesterol oxidase susceptibility of cholesterol and 5-androsten-3...

Ngày tải lên: 07/03/2014, 00:20

... semantic, and 540 positional features that have been employed by high- performing resolvers such as Ng and Cardie (2002) and Yang et al. (2003), as described below. Lexical features. Nine features ... society, island, community LOCATIO N dry land, region, landmass, body of water, geographical area, geological formation Table 1: List of keywords used in WordNet search for generating...

Ngày tải lên: 17/03/2014, 04:20

... (numbering for HEWL), for BSL2 and other lysozymes, with prop- ka 2.0 [16]. The predicted pK a values were 6.15 and 4.27 for BSL2, 5.93 and 4.20 for HEWL, and 4.89 and 3.84 for house fly lysozyme. ... (for pH 4 and 5) and sodium phosphate (for pH 6 and 7) buffer. The ionic strength of each buffer was adjusted to 0.1 [40]. Lyso- zyme solution and M. lysodeikticus suspension we...

Ngày tải lên: 23/03/2014, 04:21

... functional and structural changes of reduced and oxidized trout hemoglobins I and IV at different pHs A circular dichroism study Rosita Gabbianelli 1 , Giovanna Zolese 2 , Enrico Bertoli 2 and Giancarlo ... ligand dissociation and R fi T transition, both in the reduced and in the oxidized HbIV. The structural changes, monitored by CD, are compared with the peroxidative activity...

Ngày tải lên: 23/03/2014, 12:20

... func- tional and genomic features [9]. First, certain structural features of domains may facilitate their preferential proliferation in multidomain proteins. For example, the stability and folding ... and Gp_dh_N of glyceraldehyde 3-phos- phate dehydrogenase, Ldh_1 °C and Ldh_1_N of lac- tate ⁄ malate dehydrogenase, FGGY_N and FGG_C of the FGGY family of carbohydrate kinases,...

Ngày tải lên: 23/03/2014, 15:21

... oxidases. Biochim Biophys Acta 1783, 557–566. 10 Vrielink A & Ghisla S (2009) Cholesterol oxidase: bio- chemistry and structural features. FEBS J 276, 6826– 6843. 11 Ellis HR (2010) The FMN-dependent ... increased, and there has been a sharp rise in the last 20–30 years, owing to the rapid progress in molecular cloning and full genome sequencing. More recently, structural g...

Ngày tải lên: 28/03/2014, 22:20

... oxidases. Biochim Biophys Acta 1783, 557–566. 10 Vrielink A & Ghisla S (2009) Cholesterol oxidase: bio- chemistry and structural features. FEBS J 276, 6826– 6843. 11 Ellis HR (2010) The FMN-dependent ... increased, and there has been a sharp rise in the last 20–30 years, owing to the rapid progress in molecular cloning and full genome sequencing. More recently, structural g...

Ngày tải lên: 28/03/2014, 22:21

... structure and dynamics of individual alanine residues in the domain [3]. PLC-d1 is comprised of five domains; PH, EF-hand, X, Y, and C2 [4–6]. The PH domain is located at the N-terminus and is known ... active site (X and Y domain) and its substrate, PIP 2 [11,12]. A study using chimeric proteins of PLC- d1 and PLC-b1 led to the proposal that the interactions between the PH domain...

Ngày tải lên: 30/03/2014, 10:20