Báo cáo khoa học: Simultaneous improvement of catalytic acti

Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt

... Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution Eugene Rha 1 , Sujin Kim 1 , Su-Lim ... mutations from both the stability and activity mutants, and demon- strated a signiﬁcant improvement in both stability and catalytic activity (Fig. 1). As such, the activity and stability...

Ngày tải lên : 07/03/2014, 00:20

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Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... Approximately one- third of all mutations in the BTK gene are missense and some of these reduce the stability of the protein. This is exempliﬁed by mutations in the BTK motif of the Tec homology (TH) ... ancient and constitutes the second largest family of cyto- plasmic tyrosine kinases. In 1993, loss -of- function mutations in the BTK gene were reported as the cause of...

Ngày tải lên : 14/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... efflux of rhodamine 123 and TMRM was inhibited by NBD-Cl and MK571 and, to a lesser extent, by ATP depletion (Fig. 6). An alternative experimental approach to demon- strate the uptake of rhodamine ... prevented by an active efflux pump. The efflux was inhibited by 4-chloro-7-nitro- 2,1,3-benzoxadiazole (NBD-Cl) and MK571 and, to a lesser extent, by ATP depletion, indometh...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... E22K, E22Q and D23N), and the availability of a rapid and simple expression and puriﬁcation protocol will facilitate large-scale investigations of the molecular determinants of aggregation and ﬁbrillation. ... supplied with the enzyme, and contained Aba, Abb and Abcat 40 nm each, and the start and stop primers Abstart and Abstop at 600 nm each, and 200 lm each of...

Ngày tải lên : 18/02/2014, 13:20

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Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx

... consists of several kilobases of tandem repeats of the sequence 5¢-TTAGGG, including a terminal single-stranded overhang of $ 200 nucleotides. This overhang can fold into a variety of quadruplex ... the stability of tethered and free sequences. Kinetics of human telomere quadruplex folding Formation of tetramolecular quadruplex structures is fourth order with respect to...

Ngày tải lên : 06/03/2014, 09:22

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Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-speciﬁc intronic inverted repeats pptx

... stem. Studies of inverted repeats show that they may raise instability in a genome and, on the other hand, regu- late gene expression in both prokaryotes and eukary- otes. Being capable of forming ... The stem-loops and palindromes constructed by inverted repeats are involved in RNA interference, transcription initiation of genes, initiation of DNA replication and alternative...

Ngày tải lên : 07/03/2014, 00:20

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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx

... Hor ˇ ejs ˇ ı ´ 3 and Jan Konvalinka 1,2 1 Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, the Czech Republic; 2 Department of Biochemistry, Faculty of Natural ... catalytic domain (domain E) and the D domain of rat GCPII. The contribution of domains C and F to the GCPII hydrolytic activity/ inhibitor binding remains...

Ngày tải lên : 07/03/2014, 15:20

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Báo cáo khoa học: Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identiﬁed by site-directed mutagenesis pot

... atherosclerosis and acute lung injury. Both ACE and ACE2 have catalytic activity that is chloride sensitive and is caused by the presence of the CL1 and CL2 chloride-binding sites in ACE and the CL1 site ... I by tACE. (B) Cleavage of angiotensin I by ACE2. (C) Cleavage of angiotensin II by ACE2. Generation of product was determined by HPLC. Values are the mean...

Ngày tải lên : 16/03/2014, 04:20

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Báo cáo khoa học: Puriﬁcation, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

... detected three proteins bands by CBB staining and one activity band by activity staining. Proteins including cellulase activity were eluted and concentrated from the unstained gel, and the protein solution ... a number of proteins from the acetone-treated samples were detected by CBB staining, only one band was detected by activity staining (data not shown). After elut...

Ngày tải lên : 17/03/2014, 10:20

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Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt

... design of GCPIII-speciﬁc therapeutics and ligands, as well as in the investigation of the diverse biological functions of GCPIII and GCPII. Experimental procedures rhGCPIII and rhGCPII expression and ... Source, Argonne National Laboratory. The use of the Advanced Photon Source was supported by the US Department of Energy, Of ce of Science, Of ce of Basic Energy Scie...

Ngày tải lên : 23/03/2014, 05:22

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