Báo cáo khoa học: Spectroscopic investigation of the reaction mechanism of CopB-B, the catalytic fragment from an archaeal thermophilic ATP-driven heavy metal transporter potx

... 2009 FEBS Spectroscopic investigation of the reaction mechanism of CopB-B, the catalytic fragment from an archaeal thermophilic ATP-driven heavy metal transporter Christian Vo ¨ llmecke, Carsten ... of cuvettes with an optical path length of less than 10 lm is imperative due to the high absor- bance of water in the infrared region. Under these...

Ngày tải lên: 07/03/2014, 00:20

... rise (K from now on) and the reaction velocity (∝ from now on) was first calculated under the assumed temperature of concrete placement and then the values were corrected and re-analyzed. The final ... similar to each other, comparing to the value analyzed, the period of radiation was somewhat shorter than the value analyzed. This can be assumed that the amount...

Ngày tải lên: 07/03/2014, 03:20

... system. Spectroscopic analyses of the molecular features of the heme–GmHO-1 complex and of the mechanism of heme degradation were per- formed, and the results were compared with those for the heme ... splitting constants of the 15 N nucleus of the distal NO and of the 14 N nucleus of the proximal His of the GmHO-1 complex are closer to those of the...

Ngày tải lên: 19/02/2014, 05:20

... fluorogenic synthetic peptide substrate. These obser- vations highlight the importance of the amino acids in the transmembrane segment and also those preceding the catalytic serine in the sequence of SpsB. ... in the case of LepB, SipS and Spi. A comparison of the site of cleavage of SpsB with that of Spi from S. pneumoniae shows that they are cleaved at the s...

Ngày tải lên: 07/03/2014, 01:20

... ferrous state of the mutant may contribute to an increase in the disorder and hence entropy of the reduced state of the mutant compared to that of the wild-type protein. In contrast to the present ... that of the wild type, % 11.1 [18]. Finally, comparison of the X-ray structures of the oxidized forms of the native [5,19] and the T171S mutant (protein d...

Ngày tải lên: 07/03/2014, 21:20

... committee. Prior to introduction of CPOE, local standards of prescribing existed specifying the tenets of good practice, including the avoid- ance of the use of abbreviations. An ME was defined to have ... this study the pharmacist attended the ward round throughout the study. No other significant organisational changes occurred during the study period. The only po...

Ngày tải lên: 25/10/2012, 10:39

... homologues. Pantothenate synthetases (PSs) from all species cata- lyze the condensation of a molecule of b-alanine with a molecule of pantoate in an ATP-dependent manner to form pantothenate [1,2]. Pantothenate ... pantothe- nate from low amounts of pantoate [9]. Here, we report the results of the structural studies of the panto- ate-bound form of the N-terminal...

Ngày tải lên: 16/02/2014, 09:20

... systematic scanning of the CDR3 loops of mAb4E11 by mutagen- esis of their residues into alanine (Ala scanning). The affinities of the purified mutant Fab fragments of mAb4E11 for its antigen were ... distinguish between these mechanisms, we predicted the structural classes of the CDRs for the Fab4E11 fragment of the wild type and analyzed the potential effects...

Ngày tải lên: 19/02/2014, 07:20

... methylation [8], and the cleavage of DNA by alkali [31]. The method allows easy detection of DNA–protein interactions via the N7 position of guanines in the major groove and via the N3 position of adenines ... in hormone-induced transcription ( 50% of full induction when added early, compare lanes 3, 4 and 11, 12). Neither of these effects depended on the TSA concentr...

Ngày tải lên: 19/02/2014, 12:20

... using the second-derivative maximum of each amplification reaction and relating it to its respective stand- ard curve. The results from the quantitative PCR were expressed as the ratio of the mean ... hybridization and washing were performed according to the instructions of the manufacturer of the membrane (Amersham). The washed membrane was ana- lyzed by Phosphor...

Ngày tải lên: 20/02/2014, 01:20