Báo cáo khoa học: Donor and acceptor substrate selectivity among plant glycoside hydrolase family 32 enzymes doc

... ARTICLE Donor and acceptor substrate selectivity among plant glycoside hydrolase family 32 enzymes Wim Van den Ende 1 , Willem Lammens 1,2 , Andre ´ Van Laere 1 , Lindsey Schroeven 1 and Katrien ... 2009) doi:10.1111/j.1742-4658.2009.07316.x Plant family 32 glycoside hydrolase enzymes include hydrolases (cell wall invertases, fructan exohydrolases, vacuol...

Ngày tải lên: 07/03/2014, 00:20

... erythrocytes and calf spleen with both Xao and Xan is in the range 5–6, whereas those with Guo and Gua, and Ino and Hx, are in the range 7–8. The pH-dependence of substrate properties of Xao and Xan ... [21]. pH-dependence of substrate properties Figure 2 exhibits the substrate properties of Xao and Xan with the human and calf enzymes over the pH range 3.6–8.7, and, f...

Ngày tải lên: 31/03/2014, 09:20

... containing 81 potential substrates or regulatory ligands for enzymes in the human nucleotide metabolism. These were selected as substrates, intermediates and products of other enzymes in the human ... thermal-melt assay. PRPP, IMP and GMP were the top hits and therefore were identified as potential substrates, prod- ucts or regulatory ligands for PRTFDC1. The catalytic efficiency and...

Ngày tải lên: 15/02/2014, 01:20

... TOCSY and HSQC spectra. Figure 6 summarizes our analysis. In the COSY spectrum (Fig. 6A), the correlation between 3-CH and 4-CH2 (2.53 p.p.m. and 2.19 p.p.m.), and the correla- tion between 3-CH and ... collection and interpretation of the NMR data. Their spectra were recorded in CDCl 3 and COSY spectra lacked fine detail. HSQC and expanded COSY were not provided by Guryev et a...

Ngày tải lên: 18/02/2014, 17:20

... (b/a) 8 -barrel domain exist in these enzymes [8–10]. The relationship of sequence and structure to substrate specificity i n family 13 enzymes, particularly a-amylase, cyclomaltodextrinase, and neo pullulanase, ... identifying the region and residue important in the determination of the substrate specificity of a-glucosidases. The identification of V216T and doubly and triply...

Ngày tải lên: 07/03/2014, 16:20

... fIXa–EGR and fX, and the binding of fX is unaffected by fIXa. A dose-dependent increase in the binding of fIXa– EGR and fX accompanying an increase in the bound fVIII (Fig. 2A and D, respectively) and ... in Fig. 1B) and was fitted with a one-site model. We found that fVIII and fX induced the appearance of additional 1810 ± 370 and 541 ± 67 high-affinity sites for fIXa, respecti...

Ngày tải lên: 23/03/2014, 11:20

... after monocotyledon and dicotyledon divergence, a fact that may explain: (a) the variable length of introns among different species and between gene family members of the same plant; and (b) the absence ... saturation in LB medium, and used to transform wild-type A. thaliana plants (T 0 plants) by the floral dip method [61]. Transformants (T 1 plants) were selected on Murashige a...

Ngày tải lên: 30/03/2014, 09:20

... voltage- and Ca 2+ - activated potassium channels in the Ca 2+ store. The potassium channel was identified as the big or maxi-K (BK)-type, and was activated by posit- ive shifts in luminal potential and ... voltage-clamped at )10 mV (negative inside the pipette) and stained with DiOC 5 (3). (A) The command voltage (pipette potential) was changed from )10 mV to +30 mV and )70 mV. (B)...

Ngày tải lên: 30/03/2014, 10:20

... Zn-dependent alkylsulfatases [2] and the broad family of arylsulfatases [3]. This latter family of enzymes, termed ‘sulfatases’ in this article, is certainly the most widespread among bacteria, some of ... at  320 and  400 nm). Moreover, the extinction coeffi- cients at 400 nm mirror the Fe determinations and indicate 1.6 ± 0.2 and 1.1 ± 0.2 [4Fe-4S] 2+ clusters per monomer f...

Ngày tải lên: 16/02/2014, 14:20

... test potentials shown, and substrate- dependent currents were recorded as the difference measured in the absence and in the presence of substrates. Labeled high-affinity substrate for peptide transporters ... commonly used and best known reference substrate of H + ⁄ peptide cotransporters is [ 14 C]glycine- sarcosine ([ 14 C]Gly-Sar). This substrate is relatively stable against i...

Ngày tải lên: 07/03/2014, 05:20