Báo cáo khoa học: The capsid protein of human immunodeficiency virus: interactions of HIV-1 capsid with host protein factors ppt

... of HIV-1 capsid FEBS Journal 276 (2009) 6118–6127 ª 2009 The Authors Journal compilation ª 2009 FEBS 6127 MINIREVIEW The capsid protein of human immunodeficiency virus: interactions of HIV-1 capsid ... 2009) doi:10.1111/j.1742-4658.2009.07315.x HIV-1 is a retrovirus that causes AIDS in humans. The RNA genome of the virus encodes a Gag polyprotein, which is...

Ngày tải lên: 07/03/2014, 00:20

... but not with the D105G variant of rMASP-2 and the Y59A variant of rMAp19, is consis- tent with the fact that the variants lack the ability to associate with rMBL [56,57]. In the same way, the fact that ... [24–31]. These affect the oligomer- ization of the protein, indirectly affecting the associa- tion with the MASPs, or directly affecting the binding s...

Ngày tải lên: 07/03/2014, 05:20

... stimulation, at either 1 h or 3 h (supple- mentary Fig. S1). As qRT-PCR could not exclude the possibility of release of pre-existing IL-6, we further monitored the secretion of IL-6 protein by ELISA. ISO ... could not increase IL-6 protein production as compared with control within 3 h in the medium of cultured cardiomyocytes (supplementary Fig. S2). Therefore, there may b...

Ngày tải lên: 23/03/2014, 07:20

... independent proteins and peptides leads to the disassembly of the spherical capsid inside the virion. Some of the folded domains of Gag are then able to reassemble as inde- pendent proteins. The matrix protein ... diverse CA–CA interactions and inter- faces involved either in the assembly of the immature HIV-1 capsid, or in the reassembly of CA into a mature...

Ngày tải lên: 18/02/2014, 06:20

... 2009) doi:10.1111/j.1742-4658.2009.07314.x The mature capsid of human immunodeficiency virus, HIV-1, is formed by the assembly of copies of a capsid protein (CA). The C-terminal domain of CA, CTD, is able to homodimerize and most of the ... Mascarenhas AP & Karin Musier-Forsyth K (2009) The capsid protein of human immunodeficiency virus: interacti...

Ngày tải lên: 18/02/2014, 06:20

... forms of the MAPKs were produced as GST-lofMPK3 and GST- lofMPK6. However, when lofMPK3 and lofMPK6 proteins were tested for phosphorylation with OXI1, no phosphorylation of lofMPK3 or lofMPK6 ... mixed with GST alone as a control or with GST-tagged proteins. (B) The HIS-PTI1-4 protein was mixed with GST alone as a control or with GST-tagged proteins. In (A) and (B), the...

Ngày tải lên: 14/02/2014, 19:20

... [10]. Although the occurrence of family 33 CBPs has been known for some time [23], the present results provide only the third demonstration of the accessory function of these proteins. The effect of LlCBP33A ... drawn for ChiC1 from S. marcescens. The fact that the longer part of the substrate extends towards the + side of the catalytic centre is compatible wi...

Ngày tải lên: 18/02/2014, 08:20

... aB-crystallin (i.e. the effect of the additive on the aggregation of the target protein is inde- pendent and different from that of the chaperone). The standard errors associated with this theoretical ... (see below). The effect of the additives on aggregation of the target protein (in the absence and presence of aB-crystallin) was assessed at the end...

Ngày tải lên: 18/02/2014, 16:20

... represents the co-operativity of the transition, and [D] 50% is the concentration of urea at the midpoint of unfolding, i.e. the concentration of urea required to denature 50% of the protein. Intersection ... [33,34]. Discussion According to the prion hypothesis, a key molecular event in the pathogenesis of prion diseases is the conversion of the normal cell...

Ngày tải lên: 18/02/2014, 17:20

... 17% of residues within this stretch. We replaced the critical tri-glycine within the pea Toc75 transit peptide with repeats of these three Fig. 3. Time course of the distribution of imported proteins. ... specificity in vitro. Replacement of the most C-terminal segment with alanine residues resulted in mistargeting the protein to the stroma, while exchange of eith...

Ngày tải lên: 19/02/2014, 07:20