Báo cáo khoa học: Purine nucleoside phosphorylases from hyperthermophilic Archaea require a CXC motif for stability and folding pot

... Purine nucleoside phosphorylases from hyperthermophilic Archaea require a CXC motif for stability and folding Giovanna Cacciapuoti, Iolanda Peluso, Francesca Fuccio and Marina Porcelli Department ... catalytic activity measurements performed under standard condi- tions. Reactivation assay of SsMTAPII, PfPNP and their CXC- lacking mutants The activity of SsCSC...

Ngày tải lên: 07/03/2014, 00:20

... and water ad libitum. Membrane preparations Adrenal membranes were obtained as described previously [44]. The procedure was performed in a cold room, and all labware was washed with 1 mm EDTA ... Polyclonal antibodies against native weak toxin Naja kaouthia discriminate native weak toxins and some other three-fingered toxins against their denaturated forms. Toxicon 46, 24–30. 40 Evenas...

Ngày tải lên: 23/03/2014, 05:22

... extracted to form a potential chunk grammar and the adjacent tree structures are also extracted to form a potential context chunk grammar. After the training process, total 10937 chunk grammar ... Extracting Noun Phrases from Large-Scale Texts: A Hybrid Approach and Its Automatic Evaluation Kuang-hua Chen and Hsin-Hsi Chen Department of Computer Science and Information En...

Ngày tải lên: 31/03/2014, 06:20

... the unfolding curve was calculated [55]. Acknowledgements The authors wish to thank Linda Fahrni for technical assistance and Dr Julian Ihssen and Dr Matthijs De Geus for critically reading the manuscript. References 1 ... 55, 10446–10451. 31 Gandia-Herrero F, Garcia-Carmona F & Escribano J (2004) Purification and characterization of a latent poly- phenol oxidase from beet ro...

Ngày tải lên: 06/03/2014, 11:20

... regulation of Ab production, and an increase in tau hyperphosphoryla- tion. Furthermore, an increase in extracellular metals can catalyse Ab oligomerization and aggregation, and the amyloid plaques ... may therefore repre- sent a potential therapeutic strategy for preventing the tau hyperphosphorylation and NFT formation characteristic of AD. Modulation of metal availability for...

Ngày tải lên: 07/03/2014, 10:20

... substrate for all enzymes were smallest for t he 6¢-deoxy ana log and largest for the 2¢-deoxy analog, while intermediary losses in activity for 3¢ -and4 ¢-deoxy analogs did show small variations among ... absorbances were read at 450 nm after 4 h incubation at room temperature, and quanti®ed using the relevant deoxygenated D -glucose as standard. The a- glucosidase catalyzed hyd...

Ngày tải lên: 23/03/2014, 21:21

... glyco- saminoglycans, keratan sulfate and chondroitin 6-sul- fate, mainly in bone and cornea [1]. Clinical manifestations vary from severe to attenuated forms characterized by systemic skeletal dysplasia, laxity ... PH, Parthasarathy S, Isaacs J, Vijay S, Kieran J, Powell SK, McClelland A & Wright JF (2003) Quantification of adeno-associated virus par- ticles and empty capsids by...

Ngày tải lên: 29/03/2014, 21:20

... of this archaeon, indicating the existence of an alternative and unknown N 2 OR. This hypothesis was also raised for other bacterial and archaeal strains that reduce nitrous oxide and lack identified ... oxidase from P. aerophilum). Indeed, whereas MCOs, both laccases and metallo-oxidases, are well characterized in eukaryotes and bacteria, only one archaeal laccase has been descr...

Ngày tải lên: 18/02/2014, 04:20

... were performed. NOVOstar data parser Java data model Spreadsheet (data and metadata) KineticsWizard Instrument independent SABIO-RK MeMo-RK Experimental data + meta data Parameters + meta data Web/web ... search capability for kinetic data and corresponding metadata stored in SABIO-RK. The task of automatically finding para- meters and associated data is aided by specifying and stori...

Ngày tải lên: 18/02/2014, 04:20

... induces a conformational change that exposes a nuclear localization signal (NLS), leading to nuclear localization of NFAT, its binding to DNA, and maximal transcriptional activity. NES, nuclear export ... L, Nardone J & Rao A (2003) Tran- scriptional regulation by calcium, calcineurin, and NFAT. Genes Dev 17, 2205–2232. 50 Gwack Y, Sharma S, Nardone J, Tanasa B, Iuga A, Srikan...

Ngày tải lên: 18/02/2014, 08:20