Báo cáo khoa học: PI3K⁄Akt signalling-mediated protein surface expression sensed by 14-3-3 interacting motif pot

... promote surface expression of the chime- ric potassium channel fused with the SWTY sequence. The expression of the dominant negative Akt inhibited the enhancement of surface expression by fetal ... surface expression of reporter membrane protein. Among them, the C-terminal ‘SWTY’ peptides (represented by RGRSWTY-COOH) were found to mediate surface expression by inter...

Ngày tải lên: 07/03/2014, 00:20

... inhibited by dominant-negative Ga i protein constructs [11]. G proteins are located down- stream of G -protein- coupled receptors (GPCR) [12]. GPCR represent a large family of cell -surface proteins mediating ... heterotrimeric form [15]. Regulators of G -protein signalling (RGS) interact directly with the G protein a subunit in order to inhi- bit G -protein signalling [16]. RGS prot...

Ngày tải lên: 18/02/2014, 13:20

... olding of a small protein. J. Mol. Biol. 274, 588–596. 49. Urfer, R. & Kirschner, K. (1992) The importance of surface loops for stabilizing a n eightfold beta alpha barrel protein. Protein Sci. ... identified a Ôweak spotÕ or a Ônucleation site for unfoldingÕ in chitinase by mutational analysis. Because of the decreased number of hydrogen bonds, loo p regions at the surface of t...

Ngày tải lên: 16/03/2014, 18:20

... affinity purification followed by MS. Still, in a systematic exploration of protein complexes in the yeast interactome by tandem affinity purification fol- lowed by MS [2], the protein proliferating cell ... and 1GIY) is shown, with the rRNA molecules represented by thin coils, the tRNAs by spheres, the mRNA by a thick coil, and the proteins by ribbons. In the two views shown, t...

Ngày tải lên: 14/02/2014, 19:20

... amounts of total protein (6 lg) were loaded and the expression of MT1-MMP (pro and active) was analyzed by IB. Protein loading was controlled using the b-actin polyclonal antibody. (D) Expression of ... cell surface. To test this hypothesis, we generated an enhanced green fluorescent protein (EGFP)-furin ICD construct in which the extracellular domain of the proprotein-con- vertas...

Ngày tải lên: 18/02/2014, 04:20

... activity was created by expressing green fluorescent protein from a ColE1 plasmid, wherein rop activity is reported by cellular fluorescence. By expressing green fluorescent protein from the araBAD ... displayed proteins are typically sorted by binding to an immobilized ligand. (C) The selected proteins are then identified by isolation of DNA from cells or phage, or RT-PCR of RNA li...

Ngày tải lên: 19/02/2014, 12:20

... of protein kinase C (a, b,andf)byinsulinin3T3/L1 cells. J. Biol. Chem. 272, 2551–2558. 12. Standaert, M.L., Galloway, L., Karnam, P., Bandyopadhyay, G., Moscat, J. & Farese, R.V. (1997) Protein ... hepa- tocytes: the DAG-dependent protein kinase C pathway is not acitivated by epidermal growth factor (EGF), but is involved in mediating the enhancement of responsiveness to EGF by...

Ngày tải lên: 20/02/2014, 02:21

... Unkempt proteins. (A) Sequence alignment of human, mouse and drosophila Unkempt proteins. Triple identity is shown in red, double identity in orange. Zinc finger and RING finger motifs are indicated by ... on cobalt beads and immu- noblotted with anti-HA IgG. The expression of transfected proteins was monitored on total protein extracts by immunoblotting using the indicated antibodies....

Ngày tải lên: 06/03/2014, 09:22

... [8,9]. Localization of different proteins within living cells became possible with the fusion of a fluorescent protein, e.g. green fluorescent protein, to the protein of interest [10]. Fluorescent protein domains ... group of conformational diseases characterized by the structural conversion of native protein to alternative conformations [1]. The protein- only hypothesis states that...

Ngày tải lên: 06/03/2014, 11:20

... activi- ties by the functional motifs of the high-mobility-group chromosomal proteins. Mol Cell Biol 19, 5237–5246. 17 Bustin M & Reeves R (1996) High-mobility-group chro- mosomal proteins: ... M (2003) Chromosomal protein HMGN1 enhances the rate of DNA repair in chromatin. EMBO J 22 , 1665–1675. 15 Bustin M (2001) Chromatin unfolding and activation by HMGN(*) chromosomal proteins....

Ngày tải lên: 07/03/2014, 00:20