Báo cáo khoa học: Small-molecule modulators of zymogen activation in the fibrinolytic and coagulation systems pptx

... 3675 prothrombin (insertion of N-terminal Ile or Val into N-terminal binding cleft of the catalytic domain, resulting in conformational activation of the substrate binding site and oxyanion hole ... of thrombin from its zymogen prothrom- bin is the central event in the coagulation cascade. In addition to the conversion of fibrinogen to fibrin, thrombin orchestr...

Ngày tải lên: 06/03/2014, 22:21

... effect of inhibitor on the trypsin- catalyzed zymogen activation, the kinetic parameters of trypsin-catalyzed zymogen hydrolysis in the absence of inhibitor were determined first. The trypsinogen ... the effects of acetamidine, p-amidino- benzamidine and benzamidine on the autoactivation of trypsinogen by trypsin. Figure 4 shows time courses of trypsinogen auto...

Ngày tải lên: 23/03/2014, 13:20

... in obese mice In order to gain insights into the potential biologically relevant role of miR-27 in the regulation of adipose tis- sue functions in vivo, we examined the expression of miR-27 in ... differentiation was initiated at 48 h post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were fixed on day 4 of differentiation...

Ngày tải lên: 18/02/2014, 08:20

... identified prothrombin as one of the plasma cofactors. In incubations of U937 cells with prothrombin and Xa, plactin increased the formation of thrombin, which cleaved single-chain u-PA to afford the inactive ... 4). These properties of the plactin–prothrombin interaction suggested a change in the conformation of prothrombin after plactin bind- ing. We measured the...

Ngày tải lên: 07/03/2014, 00:20

... to 30 kDa in size. Subunits of all known GST structures exhibit a two-domain fold, the N-terminal domain and C-terminal domain, including the highly conserved GSH-binding site (G-site) and the more ... differ in only three amino acids: the triad Arg89, Glu117 and Ile172 found in the isoform GSTU1 is replaced by the triad Pro89, Lys117 and Val172 in the GSTU2 is...

Ngày tải lên: 15/03/2014, 09:20

... the sixth transmembrane domain and the third extracellular loop playing a promin- ent role. Interestingly, the N-terminus and a segment between the fourth transmembrane domain and the third intracellular ... role in the FPRL1 response to MMK-1. Based on the deduced amino acid sequences of FPR and FPRL1, the seg- ment spanning the C-terminal half of the first T...

Ngày tải lên: 16/03/2014, 18:20

... acetyl-transferase-catalyzing inner domain. E3BP domains: L3, N-terminal lipoyl domain; B, E3 binding domain; I, inner domain which associates with the inner domain of E2. Dotted connections indicate binding interactions ... running in opposite direc- tions. The monomer association involves the ATP-using C-terminal domains interacting near the base of that side of the wedge...

Ngày tải lên: 17/03/2014, 09:20

... than the other compounds (Fig. 6). In this way, amino acid res- idues of different segments of NADP-ME2 swapped in the construction of the chimeras are involved in the fumarate activation of the ... to determine the three-dimensional structure of a plant NADP-ME in the presence and absence of the sub- strates to analyse the conformational changes that...

Ngày tải lên: 23/03/2014, 04:20

... of point mutations in the binding domain of a-2-C on the binding to c¢-2 To elucidate whether single amino acids in the binding domain of a-2-C were particularly important for the formation of ... domain in the N-terminal part and the biotin-binding domain in the C-terminal part [5]. The b-subunit is an integral membrane protein with nine membrane-spanning a-...

Ngày tải lên: 23/03/2014, 13:20

... an indirect role in reproduction. It is possible that the introduction of in- chain hydroxyl allows cross-linking of cutin, leading to reticulation and strengthening of the polymeric envelope. The ... demonstrated the involvement of FA hydroxy- lases in the synthesis of the protective biopolymers cutin, suberin and sporopollenin. In addition, several lines of...

Ngày tải lên: 22/03/2014, 16:21