Báo cáo khoa học: Specific cleavage of the DNase-I binding loop dramatically decreases the thermal stability of actin pot
Báo cáo khoa học: Specific cleavage of the DNase-I binding loop dramatically decreases the thermal stability of actin pot
... [6,27,43,44]. Thus, the increased thermal stability of G -actin appears to correspond to the closed conformation of the nucleotide -binding cleft, whereas the decreased thermal stability is a feature of the actin ... studied the effects of the D -loop cleavage on the thermal unfold- ing of G- and F -actin. Previously, the thermal unfold- ing of...
Ngày tải lên: 06/03/2014, 22:21
Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
... phospholipid and membrane binding of the other members of the PEBP family. Consequently, the binding characteristics of PEBP proteins, including those of the binding of I C to phospholipid membranes, are ... site of I C overlap, and that the N-ter- minal segment at the CPY -binding sites participates in regulation of the specific binding of I C to the...
Ngày tải lên: 19/02/2014, 05:20
Báo cáo khoa học: Specific degradation of H. pylori urease by a catalytic antibody light chain pptx
... recognition specificity, these results demonstrated that the cleavage could take place at multiple sites. We observed that the main digestion of the urease by HpU-9-L was initiated by the cleavage of the ... red arrows; the blue underlines are the assumed cleavage sites based on molecular sizes and sequencing. The main digestion of the urease by HpU-9-L was initi-...
Ngày tải lên: 07/03/2014, 21:20
Tài liệu Báo cáo khoa học: Specific interaction between the classical swine fever virus NS5B protein and the viral genome pdf
... nucleotide bases at the t erminal of the 3¢ UTR. Therefore, the 3¢ UTR, specifically the 3 ¢ terminal region, is very important fo r binding of the CSFV NS5B protein, whereas the sequences of the c oding ... the same time, the EMSA was carried out under the condition of a fixed amount of the NS5B and the radiolabeled +RNA0 in the presence of increasing a...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast docx
... the first division time showed large variations as a result of the diverse cell cycle points at the time of the glucose concentration shift. The mean values (3.5–3.8 h) for the doubling time of ... cytokinesis. The positions of septation were often not precisely at the equator in dividing cells in 4.4 and 2.2 mm glucose (Fig. 3A). The relative standard deviation of...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Specific Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling pdf
... with the b-Trp )8 residue. SWISS-MODEL structure modeling sug- gests the presence of a specific coordination motif of Ca 2+ at the C-termi- nus of the a-subunit of LH1, while MODELLER reveals the ... a molecules. The results are discussed in terms of the long-wavelength Q y absorption of native LH1–RC. Furthermore, the systematic shift of fluorescence spec- tra a...
Ngày tải lên: 30/03/2014, 02:20
Báo cáo khoa học: Specific TSC22 domain transcripts are hypertonically induced and alternatively spliced to protect mouse kidney cells during osmotic stress pptx
... distinct isoforms from a single gene by use of different combi- nations of splice junctions. For example, alternative splicing within the DNA -binding domain of Pax-6 alters DNA -binding specificity of the ... cells We analyzed the expression of the six mouse TSC22D transcripts in kidney to learn whether any of them functionally resembles tilapia OSTF1. Levels of expres...
Ngày tải lên: 30/03/2014, 10:20
Báo cáo khoa học: b-Secretase cleavage is not required for generation of the intracellular C-terminal domain of the amyloid precursor family of proteins pot
... inhibition of BACE1 will not adversely affect the potentially important signaling role of the ICDs released by the APP family of proteins, but may impact on other functions of this family of proteins. Experimental ... charac- terize the impact of BACE1 inhibition as a putative therapy for the treatment of AD. We found that the de novo production and the endogenous...
Ngày tải lên: 15/03/2014, 10:20
Tài liệu Báo cáo khoa học: Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form ppt
... at the A surface passing through the hole on the other side of the helix and the other at the D surface passing through the C surface on the other side of the helix. Table 1. Mutations of the ... perpendicular to the helix axis. One passes through the C and D surfaces on opposite sides of the helix and the other passes through the A surface and a prominen...
Ngày tải lên: 19/02/2014, 00:20
Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
... energies with the rest of the complex are larger than in the case of substrates with the polar group oriented towards ArgA145. The interaction energy of ArgA145 itself with the rest of the complex ... constant of the same order of magnitude as one of the most specific substrate of PA–PG. Phenylacetyl-4-methylcoumaryl-7-amide (PhAc-MCA) has a specificity const...
Ngày tải lên: 16/03/2014, 16:20