Báo cáo khoa học: In vivo production of catalase containing haem analogues pot

... porphyrins. For the in vivo production of catalase containing haem analogues, we grew E. faecalis strain V583 ⁄ - pLUF15, which overproduces catalase polypeptide, in the presence of the respective haem ... and incorporated into vital haem proteins instead of haem [5]. We show here that haem analogues are indeed taken up and can be incorporated into haem prot...

Ngày tải lên: 06/03/2014, 11:20

... pharmacological vitamin A supplementation may increase the risk of adverse effects including the risk of oncogenesis. Keywords: vitamin A; retinoic acid; p53; cyclin D1; c-Jun 6 . Vitamin A (retinol) is an ... m M dithiothreitol in a Perkin-Elmer Gene- Amp 9700 thermal cycler at 25 °C(10min),50°C (60 min) and 70 °C (15 min). The PCR was started by adding 2 lL of the cDNA solution to a...

Ngày tải lên: 08/03/2014, 08:20

... previously to induce an increase in the level of [Ca 2+ ] i and a correlated calpain activation [28]. This increase in [Ca 2+ ] i is more intense in aorta than in brain. Under these conditions, in brain, ... FEBS higher than in aorta, resulting in a much higher HSP90 to NOS ratio in brain (Fig. 1C). Calpain activation in rat brain and aorta following HSD treatment To promo...

Ngày tải lên: 30/03/2014, 04:20

... cross-linking in vivo. Experimental procedures Collection of sperm Specimens of A. pectinifera were collected, during their breeding season, from the coastal waters off Japan and were maintained in ... role in catalyzing protein cross-linking reactions via the formation of an N e (c-glutamyl)lysine bond between the c-carboxyl group of a Gln residue in one polypeptide chain a...

Ngày tải lên: 31/03/2014, 07:20

... developed in the present study represents an important technical advance for examining the first crucial step in success- fully using in vivo RNAi: the introduction of dsRNA into animal tissues. In vivo ... the gonad with 100 lL of saline solution containing dsRNA, or saline solution for the con- trol. After dsRNA injection, oysters were maintained in raceways in conditio...

Ngày tải lên: 07/03/2014, 00:20

... formed. To gain insight into the binding of drugs to bulge DNA, several bulge- containing oligonucleotides were selected as binding hosts for DDI-1A and DDI-1B. CD spectroscopy of DDI-1A (Fig. ... by using 32 P-labeling primer or template in the presence or absence of DNA-binding agents (DDIs and doxorubicin). The DNA bulge binding of both compounds was detected by CD and UV melti...

Ngày tải lên: 07/03/2014, 06:20

... synchronisation of text and speech • building an efficient closed caption production system The outline of the system is shown in Figure 1. Although all types of TV programs are to be handled in the ... 1997). 3.1.2 Rules for shortening text Another way of reducing the number of characters in a Japanese text, thus summarising the text, is to shorten or delete parts...

Ngày tải lên: 08/03/2014, 06:20

... membrane properties. As in past reports, the j oint use of mutants and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the in uence of d irect effects, ... was observed, and this was indepen dent of the kind of genetic lesion or inhibitor used, suggesting that this activating effect was indeed caused by the fatty acid/lipid enviro...

Ngày tải lên: 08/03/2014, 22:20

... not bind the same single binding site, rather that I-AniI appears to contain two binding sites. Keywords: Aspergillus nidulans; homing endonuclease; RNA binding protein; DNA sliding; RNA splicing. Group ... rate constant for the binding of DNA to protein kinetically and obtained a value of at least half this in TK8 buffer containing 10 m M MgCl 2 [33]. Therefore, given that the DNAÆpr...

Ngày tải lên: 17/03/2014, 10:20

... AASDH, containing a PQQ binding domain, is supposed to be involved in lysine degradation and to convert the a-aminoadipate semi- aldehyde to a-aminoadipate [12]. Lys2, possessing a NADPH-binding ... N-terminal domains are homologous to the A and T domains from NRPS systems and the C-terminal part contains a redox cofactor binding site for either pyrroloquinoline quinone (PQQ) or NADPH. I...

Ngày tải lên: 23/03/2014, 13:20