Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
Báo cáo khoa học: Vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide attenuate the cigarette smoke extract-induced apoptotic death of rat alveolar L2 cells ppt
... aliquots at )20°C until used. On the day of the experiment, one aliquot of the stock solution was thawed and diluted in buffer to the appropriate concentration. RT-PCR analysis of mRNAs encoding PACAP/VIP ... analysis of the saturation data [23]. The ability of VIP and PACAP27 to inhibit the specific binding of 125 I-labeled VIP (0.03 n M )was estimated from th...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Human-blind probes and primers for dengue virus identification Exhaustive analysis of subsequences present in the human and 83 dengue genome sequences doc
... of the virus which is common in that region, a re-emergence of an earlier version, a continuation of an outbreak from the previous season or the introduction of a new strain as the result of Fig. ... 3. Distribution of the percentage of n-mers per genome that are unique (i.e. not contained in any of the other dengue genomes considered). Fig. 4. Distribution of t...
Ngày tải lên: 23/03/2014, 11:20
Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
... analysis, and the determination of released ammonia. The coupled enzyme assay revealed the mechanism of the deamination reaction and the subsequent metabolism, including the deamination step. The product ... one enzymatic and one nonenzy- matic, see below) to 2-hydroxymuconic 6 -semialdehyde and an almost equimolar concentration of ammonia. The Fig. 2. Ab sorptio...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: A novel metallobridged bis(b-cyclodextrin)s fluorescent probe for the determination of glutathione doc
... bis(b-CD)s. Because of the conformation of the linker of 1, the nitrogen atoms and amido bond formed two chelate rings to coordinate with copper (II) ions. This coordi- nation effect and the paramagnetism of ... metallobridged bis(b-CD)s 2 The influence of the concentration of 2 on fluorescence intensity is shown in Fig. 4. As can be seen, as the concentration o...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Cofactor-independent oxygenation reactions catalyzed by soluble methane monooxygenase at the surface of a modified gold electrode pot
... modulating the rate of electron transfer between MMOR and MMOH [4,5], altering the redox potential of the binuclear iron site [6], optimizing the interaction between MMOR and MMOH [7], controlling the ... investigate the effects of catalase, MMOB and MMOB tru and the substrate acetonitrile on electron transfer to MMOH was also performed between 0 and )0.6 V, at...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: From functional genomics to systems biology Meeting report based on the presentations at the 3rd EMBL Biennial Symposium 2006 (Heidelberg, Germany) Sergii Ivakhno pot
... functional association was further aided by integration of protein interaction data with data on gene expression, localization, func- tion, evolutionary conservation, protein structure and binary interactions. ... [15] and yeast [16], consist of physical and functional inter- actions between TFs and their target genes represented on the graph [17]. The systematic mapping...
Ngày tải lên: 30/03/2014, 09:20
Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... MINIREVIEW Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information Mitsuo Umetsu 1,2 , Takeshi ... or NMR structural analyses and the construction of artificial libraries allow con- structive selection and functionalization not only of antibody fragments, but also of small, n...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: Krit 1 interactions with microtubules and membranes are regulated by Rap1 and integrin cytoplasmic domain associated protein-1 doc
... from the perinuclear region to the leading edge upon chemo- kine stimulation and activated Rap1 triggers the association of RAPL with aLb2, leading to the redis- tribution of this integrin to the ... and, for the second part, with the higher contribution to the binding to MT of the basic stretch than the C-terminal site. At the cell periphery, Rap1 shuttles betw...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: Coiled–coil interactions modulate multimerization, mitochondrial binding and kinase activity of myotonic dystrophy protein kinase splice isoforms pptx
... of multimerization promoted by the N terminus and the coiled-coil domain [16,17]. However, the consequences of multimerization and association with other proteins for in vivo activity regulation of DMPK ... properties of any particular amino acid segment therein [24]. We compared the localiza- tion of YFP-tagged isoforms A, C and E, containing the L477G, L487G and...
Ngày tải lên: 16/03/2014, 13:20
Tài liệu Báo cáo khoa học: SREBPs: protein interaction and SREBPs Ryuichiro Sato doc
... increase in the number of proteins modified by SUMO, it has become obvious that the effects of SUMO conjugation are diverse and largely depend on the function of the protein targeted for sumoylation. Sumoylation ... recruitment of PGC-1a. Under fasting condi- tions, HNF-4 and PGC-1a vigorously activate the expression of gluconeogenic genes. In contrast, under feedin...
Ngày tải lên: 18/02/2014, 13:20