Báo cáo khoa học: Protein–protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling doc

... indicates that at this side of the molecule either the polar interactions with its interact- ing protein were fully replaced by the amino group or that no or only minor ligand protein interactions ... hypoxanthine) and the diphospho-bridge, as they can be replaced by much simpler ether strands. These ether strands mimic the distance between the nucleo- base and the diphospho-b...

Ngày tải lên: 20/02/2014, 01:20

... various epitopes within the prion protein sequence (Fig. 1 and Table 1). The two bands of higher molecular masses are the di- and mono-glycosylated isoforms and the band with the lowest molecular ... SAF34 and P4 strongly stained the di-glycosylated band, and mAbs 6H4, SAF60, SAF70 and SAF84 showed the highest staining with the overlapping bands of nonglycosylated full-length Pr...

Ngày tải lên: 19/02/2014, 02:20

... [13]. GlnK consists of three 12-kDa monomers, and is a small regulatory protein that belongs to the PII protein family. GlnK homologs bind to AmtB and regulate its activity, depending on the cellular ... analyte in SPR analysis. ATP and 2-oxoglutarate are known to be the primary effec- tors involved in PII signaling, and they strongly affect interactions of many GlnK proteins...

Ngày tải lên: 06/03/2014, 00:21

... mutations at the surface are all hydrophilic substitutions that increase the net positive charge by seven, and enable protein protein interac- tions that are favorable for crystal formation. Of the overall ... length sup- port that idea by demonstrating that: (a) wild-type GLYAT accommodates shorter ligands (with three and four atoms in the main chain) more readily than longer...

Ngày tải lên: 14/02/2014, 14:20

... Mol-A and Mol-B, Mol-A and Mol-C, Mol-A and Mol-D are 0.77, 0.94, and 0.74 A ˚ , respect- ively, for all atoms, and 0.42, 0.55, and 0.43 A ˚ , respectively, for main-chain atoms, suggesting that ... sugar-binding protein family was found to be located upstream of the TVA II gene, and the affinities of this protein for c-CD were higher than that for maltose [7]. The result...

Ngày tải lên: 19/02/2014, 00:20

... suggests that the lower levels of secretion are due to post-transcriptional processes, and that the reduction in secreted protein is a result of degradation of partially folded or misfolded protein ... identified between the level of secreted protein and the thermo- stability of the native state of the protein (Fig. 2A). This correlation shows that even small changes in the...

Ngày tải lên: 19/02/2014, 08:20

... periplasmic proteins that are exported only after binding redox cofactors such as FeS or molybdopterin centres [5–8] although it should also be emphasized that the system also transports proteins that ... evidence for interactions between the TM spans of TatA and TatB, and between the amphipathic regions. Finally, we have re-examined the numerous mutations made previously in TatA,...

Ngày tải lên: 19/02/2014, 17:20

... abdominal segment 9 and also that of abdominal segment 10 (for examples see [1,2]). Here we focus on the transcriptional unit coding for the class A transcript, and refer to it and its regula- tory ... suggest that the PTS and the promoter sequences are incorpor- ated irreversibly into a protein- DNA complex in the germ line cells, and the established contact is main- tained th...

Ngày tải lên: 20/02/2014, 01:20

... OsHsfA2a, OsH- sfB4c and OsHsf26 proteins. The bimolecular fluorescence complementation (BiFC) technique has provided support in indicating that Hsfs show protein protein interactions. This tech- nique ... types. We further provide data on protein protein interac- tions, DNA binding characteristics and transactivation activity of selected members of class A, B and C OsHsf prot...

Ngày tải lên: 05/03/2014, 23:20

... energy expenditure. These data suggest that CPT1c can integrate carbohydrate and lipid nutrient sensing in the brain and is an example of an enzyme that can sense and respond to the nutritional environment. ... of orexigenic and anorexigenic neuropeptide expression during fast- ing and refeeding. A detailed kinetic analysis of hypo- thalamic malonyl-CoA has shown that glucose...

Ngày tải lên: 14/02/2014, 22:20