Báo cáo khoa học: Complementation of coenzyme Q-deﬁcient yeast by coenzyme Q analogues requires the isoprenoid side chain ppt

... (h)A60002468101214024487296Wild-typeDcoq2 + 50 µM CoQ2Dcoq2 + 50 µM decylQDcoq20246810A600DCoQCoQ2DecylQA1 -Q 2A4 -Q 2A5 -Q 2A6 -Q 2A3 -Q 2A2 -Q 2ABOOMeOMeOA1 -Q 2OOMeOMeOA6 -Q 2OOMeOMeOA5 -Q 2OOMeOMeOA4 -Q 2OOMeOMeOA3 -Q 2OOMeOMeOA2 -Q 2OOMeOMeODecylQOOMeOMeOCoQ2OOMeOMeOCoQ6CFig. ... ubiquinones.Results Complementation of cell growth in CoQ-deﬁcient yeast by short -chain CoQ analogues shows adramatic dependence on the isoprenoid side chain Yeast strains that cannot synthesize CoQ endoge-nously ... loss of area from the CoQ2H2and decylQH2peaks was compara-ble with the gain in size of the CoQ2and decylQ peaks.Determination of the fraction of CoQ2and decylQ thatcould be reduced by...

Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

... supershift group by KLF4 antibody. (B)Recruitment of KLF4 to the binding element of the SR-BI promoterregion. The ChIP assay was used to detect the binding of KLF4 to the SR-BI promoter. The cross-linked ... THP-1macrophages, the increment level induced by HDL3was much higher than that by HDL2. Therefore,HDL3was chosen as the stimulus in the subsequentexperiments. The expression of SR-BI was also ... HDL-cholesteroldelivery to the liver and induces susceptibility toatherosclerosis. The regulatory effect of KLF4 on SR-BI reveals a novel pathway to elucidate the mechanism of SR-BI in the development of atherosclerosis....

Báo cáo khoa học: "Impact of computerized physician order entry on medication prescription errors in the intensive care unit: a controlled cross-sectional trial"

... zeros, but most of them were double pre-scriptions, which were identified by the nurse or the physician.These types of errors did not occur in the PB-U, meaning theywere caused by the CPOE system ... immediately visible by adding a black sign. Anotherproblem was the request of drug plasma concentration levels.They were often being forgotten or, on the other hand, stillasked for when the medication ... contributionsAll of the authors were involved in designing the study. KC wasresponsible for conceiving the study, data acquisition, analysis of the data, statistical analysis and drafting of the manuscript.BC...

Tài liệu Báo cáo khoa học: Modulation of F0F1-ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels pptx

... setting, the ability of the F0F1-ATP synthase topump protons out of the matrix represents the onlycomponent opposing the action of an uncoupler. On the basis of a recent study by Giorgio ... changes, as the percentagechange in the steady-state rate of the pathway divided by the percentage change in the enzyme activity caus-ing the ﬂux change. The FCCs for ANT and mostother mitochondrial ... would bestrengthened if the FCC of the ANT is sufﬁcientlyhigher than that of the F0F1-ATP synthase for adeninenucleotide ﬂux rates. The determination of the FCCswas performed by measuring...

Tài liệu Báo cáo khoa học: Inhibition of cobalamin-dependent methionine synthase by substituted benzo-fused heterocycles pptx

... displace the dimethylbenzimidaz-ole side chain of the cobalamin-cofactor or that it mayact on the binding site of the MetS allosteric cofactor,S-AdoMet, both effects which could explain the observed ... optimization of inhibitors, a molecular model of the methyltetra-hydrofolate-binding domain of the rat liver MetS wasconstructed. In the absence of a high-resolution struc-ture of the methyltetrahydrofolate-binding ... min, the reaction wasinitiated by the addition of 500 lm (dl)-homocysteine andincubated at 37 °C for a further 30 min, unless otherwisestated. The reaction was terminated by the addition of...

Tài liệu Báo cáo khóa học: Inactivation of copper-containing amine oxidases by turnover products doc

... cuvette closed by a Teﬂon stopper. The oxygen was consumed by mostsubstrates within the mixing time as revealed by the immediate bleaching of the TPQ 500 nm band and by the appearance of absorption ... reaction, because of the broad band (shoulder) formed in the 350 nm region. By subtracting the spectrum of native LCAO from the spectrum of the radical, or from the spectra of the inactivated protein, ... spectrum and by the bleached500 nm band. Which of the two equilibrium species, the radical or the Cu2+-quinolamine, participated in the reaction is not certain. On one hand, the decay of the radical...

Tài liệu Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain ppt

... absence of Adh4mRNA in the stomach epithelia – the tissue with the bestcharacterized Adh4 expression.Taken together, our results demonstrate expression of ADH3 in most of the analyzed areas in the ... particularly the striatum,can oxidize retinol to retinal, providing the ﬁrst step on the way to retinoic acid [31]. The brain must thus rely on the activity of other enzymes, for example ADH3 or othermembers ... othermembers of the medium -chain dehydrogenase/reductasefamily (MDR), or perhaps members of the short -chain dehydrogenases/reductase family (SDR), both of whichutilize a variety of metabolites...

Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx

... inhibited by these peroxidesin the absence of added Fe2+–EDTA. The presence of thismetal-ion complex enhanced the inhibition observed withthese enzymes consistent with the o ccurrence of radical-mediated ... Furthermore removal of the peroxide groups by reduction p revents enzyme inhibitio n. Such a mechanism isalso supported by the inefﬁcient inhibition of LDH by theseperoxides, in the absence of metal ions, ... and thereby maintains reducing equi-valents within the cell. Other enzymes, such as LDH, areunaffected, so such damage is selective. The long lifetime of these protein peroxides may allow these...

Tài liệu Báo cáo khoa học: Enhancement of oxidative stress-induced apoptosis by Hsp105a in mouse embryonal F9 cells pptx

... differentiation of F9 cells were not affected by the over-expression of Hsp105a, the sensitivity of cells to oxidative stress wasenhanced by the over-expression of Hsp105a,andtheseﬁndings were ... Ig.C,cytosolicfraction;M,mitochondrialfraction. (B) The densities the cytochrome c bands were quantiﬁed by densitometry, and the rates release into the cytosol are shown. Datarepresent the means ± SD of three independent ... apoptosis at orupstream of cytochrome c release from mitochondria.Furthermore, the transmission of signals from externalstresses is accompanied by the activation of a family of stress-activated...

Báo cáo khoa học: Activation of activating transcription factor 2 by p38 MAP kinase during apoptosis induced by human amylin in cultured pancreatic b-cells ppt

... inhibitor I, by about 70%; and the combination of SB203580, the JNK inhibitor I and acaspase-8 inhibitor, by 100%. These ﬁndings demonstrate the requirementfor concurrent activation of the p38 kinase, ... molecular cloning techniques [48]. The clonedc-jun promoter sequences were veriﬁed by DNA sequencing(DNA sequencing service, Centre for Genomics and Proteo-mics, the University of Auckland, New Zealand).Luciferase ... both AP-1 and CREsequences as a heterodimer, which it can form withother members of the ATF family or with Jun ⁄ Fos fam-ily members [35,36]. The most common of these is the ATF-2 ⁄ c-Jun heterodimer...

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