Báo cáo khoa học: Tetracysteine-tagged prion protein allows discrimination between the native and converted forms pptx

... 277 (2010) 2038–2050 ª 2010 The Authors Journal compilation ª 2010 FEBS Tetracysteine-tagged prion protein allows discrimination between the native and converted forms Jernej Gas ˇ pers ˇ ic ˇ 1 , ... revolutionized protein science and cell biology. However, large and slow-fold- ing fluorescent proteins may influence the folding of their fusion partner, and, in...

Ngày tải lên: 06/03/2014, 11:20

... species is 60% among the PrP1 proteins, 50% among the PrP2 proteins, and 40% between PrP1s and PrP2s [8], one is tempted to hypothesize that with regard to their expression in E. coli and sub- sequent ... encode a protein that forms a typical prion protein 3D structure when isolated with the same purification and refolding methods that were successful with the other s...

Ngày tải lên: 30/03/2014, 04:20

... embryonic stem cell cultures Among the hot topics in current research is the genera- tion and use of stem cells. The theoretical applications of having expandable and differentiation-controllable stem ... downstream of Nodal. The MPCs are then able to integrate and interpret the signal strengths of Nodal and BMP by entering the somite regions at different somites. Strong N...

Ngày tải lên: 19/02/2014, 00:20

... known, smaller and much less active type I antifreeze proteins cannot by themselves protect the flounder down to the freezing point of seawater. The relationship between the large and small antifreezes ... protect the fish down to the freezing point of seawater. The absence of the small type I AFPs in the American plaice argues that these single helix antifreezes of the...

Ngày tải lên: 20/02/2014, 01:20

... characterized by the accumulation of amyloid in the brain in the form of amyloid plaques and cerebral amyloid angiopathy. The major component of the amyloid plaques, the amyloid-b protein (Ab), ... nonfibrillar and are unable to be converted into fibrils or to seed fibril formation [25]. The aim of the present study was to examine the effect of pH and metal ions on t...

Ngày tải lên: 31/03/2014, 07:20

... estroys the TH activity of sbwAFP [42]. The structure shows that there is a right- handed cap at the C-terminus of the protein, which forms two antiparallel sheets with b-stands from the preceding coil. ... path that the coils follow, and the b-sheets that are found on one or more faces of the protein perpendicular to the helical axis. The strands from the b-sheet...

Ngày tải lên: 19/02/2014, 16:20

... morphemes, is com- posed of the 2nd candidate at the 1st position, the 1st candidate at the 2nd position and the 3rd candidate at the 3rd position. The architecture described in the previous section ... corresponds to the position in the text. The other dimension corresponds to the candi- date level. Candidates on the same level form one stream. For example, i...

Ngày tải lên: 21/02/2014, 20:20

... e t , and the other that moves mention l  from entity e  s to entity e  t . The set of factors to compute acceptance of the first pro- posal are factors between l and mentions in e s and e t , ... Inference: Distributor divides the entities among the machines, and the machines run inference. The process is re- peated by the redistributing the entities. mawat, 200...

Ngày tải lên: 07/03/2014, 22:20

... was synthesized. The expression pattern of o2 and fl-2 depends on the molecular basis of the mutation. It remains necessary to evaluate the relationship between the expression patterns and the molecular ... genes. Among these genes, 82% of the encoded proteins have signal pep- tides. On the other hand, only two down-regulated genes, vegetative storage proteins Vsp1 and...

Ngày tải lên: 16/03/2014, 11:20

... conserved protein, the small Ras-rela- ted GTP binding protein, Gtr1, as a protein that Meh1 interacts with. To obtain further information about the function of Meh1, we examined whether or not Meh1 and ... that are normally retrieved from the Golgi and the underglycosylation of proteins normally modified in the Golgi [10]. Neither the pre- cise function of ERD1 nor the m...

Ngày tải lên: 16/03/2014, 18:20